Experiment Overview
| Repository ID: | FR-FCM-ZYBB | Experiment name: | Knapp Kannan Pellacani and Eaves-Cell Reports-2017-mammary epithelial cell lines | MIFlowCyt score: | 68.00% |
| Primary researcher: | David JHF Knapp | PI/manager: | David JHF Knapp | Uploaded by: | David JHF Knapp |
| Experiment dates: | 2016-02-06 - 2016-05-06 | Dataset uploaded: | Sep 2017 | Last updated: | Mar 2018 |
| Keywords: | [mammary epithelial cells] [mass cytometry] [Cell Signaling] | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
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| Purpose: | Mass cytometric analysis of mammary epithelial cell lines under various conditions. | ||||
| Conclusion: | Mammary cell line characteristics can be identified by mass cytometry. Complex mixtures of epithelial cells can be separated based on their mass cytometric profiles. | ||||
| Comments: | Experiment 1 has high background staining across all channels. None-the-less, profiles within the population are internally consistent and comparable to other experiments if the data is normalized. | ||||
| Funding: | D.K. was supported by Vanier Scholarship. N.K. was supported by CBCF-BC/Yukon and MITACS Elevate Postdoctoral Fellowships. Most of the antibodies used in this study were kindly provided by Fluidigm Corporation. The study was supported by grants to C.J.E. from the Canadian Cancer Society Research Institute. | ||||
| Quality control: | 4EQ Normalization beads were included in the experiment to normalize machine signal. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Growth Media | Exp1_17_MCF7_P171_media.fcs · Exp1_19_ZR75-1_P102_media.fcs · Exp1_20_HCC1937_P21_media.fcs · Exp1_BT20_P299_media.fcs · Exp1_HeLa_NA_media.fcs · Exp1_MCF10A_P27_media.fcs · Exp1_MDAMB231_P62_media.fcs · Exp1_SKBR3_P45_media.fcs · Exp1_SUM149_P37_media.fcs · Exp1_T47D_P89_media.fcs · Exp3_BT20_media.fcs · Exp3_HeLa_media.fcs · Exp3_MCF10A_media.fcs · Exp3_MCF7_media.fcs · Exp3_MDAMB231_media.fcs · Exp3_SKBR3_media.fcs · Exp3_SUM149_media.fcs · Exp3_T47D_media.fcs · Exp3_ZR75-1_media.fcs |
| · 100 ng/mL EGF 10 minutes | Exp1_20_HCC1937_P21_EGF10min.fcs · Exp1_BT20_P299_EGF10min.fcs · Exp1_HeLa_NA_EGF10min.fcs · Exp1_MCF10A_P27_EGF10min.fcs · Exp1_MCF7_P171_EGF10min.fcs · Exp1_MDAMB231_P62_EGF10min.fcs · Exp1_SKBR3_P45_EGF10min.fcs · Exp1_SUM149_P37_EGF10min.fcs · Exp1_T47D_P89_EGF10min.fcs · Exp1_ZR75-1_P102_EGF10min.fcs · Exp2_HeLa_NA_EGF10min.fcs · Exp2_MCF10A_P27_EGF10min.fcs · Exp2_MCF7_P171_EGF10min.fcs · Exp2_MDAMB231_P62_EGF10min.fcs · Exp2_SKBR3_P45_EGF10min.fcs · Exp2_SUM149_P37_EGF10min.fcs · Exp2_T47D_P89_EGF10min.fcs · Exp2_ZR75-1_P102_EGF10min.fcs |
| · Starve | Exp1_19_HCC1937_P21_starve.fcs · Exp1_BT20_P299_starve.fcs · Exp1_HeLa_NA_starve.fcs · Exp1_MCF10A_P27_starve.fcs · Exp1_MCF7_P171_starve.fcs · Exp1_MDAMB231_P62_starve.fcs · Exp1_SKBR3_P45_starve.fcs · Exp1_SUM149_P37_starve.fcs · Exp1_T47D_P89_starve.fcs · Exp1_ZR75-1_P102_starve.fcs · Exp2_BT20_P299_starve.fcs · Exp2_HeLa_NA_starve.fcs · Exp2_MCF10A_P27_starve.fcs · Exp2_MCF7_P171_starve.fcs · Exp2_MDAMB231_P62_starve.fcs · Exp2_SKBR3_P45_starve.fcs · Exp2_SUM149_P37_starve.fcs · Exp2_T47D_P89_starve.fcs · Exp2_ZR75-1_P102_starve.fcs |
| Sample Type | |
|---|---|
| · BT20 | Exp1_BT20_P299_EGF10min.fcs · Exp1_BT20_P299_media.fcs · Exp1_BT20_P299_starve.fcs · Exp2_BT20_P299_starve.fcs · Exp3_BT20_media.fcs |
| · HCC1937 | Exp1_19_HCC1937_P21_starve.fcs · Exp1_20_HCC1937_P21_EGF10min.fcs · Exp1_20_HCC1937_P21_media.fcs |
| · HeLa | Exp1_HeLa_NA_EGF10min.fcs · Exp1_HeLa_NA_media.fcs · Exp1_HeLa_NA_starve.fcs · Exp2_HeLa_NA_EGF10min.fcs · Exp2_HeLa_NA_starve.fcs · Exp3_HeLa_media.fcs |
| · MCF10A | Exp1_MCF10A_P27_EGF10min.fcs · Exp1_MCF10A_P27_media.fcs · Exp1_MCF10A_P27_starve.fcs · Exp2_MCF10A_P27_EGF10min.fcs · Exp2_MCF10A_P27_starve.fcs · Exp3_MCF10A_media.fcs |
| · MCF7 | Exp1_17_MCF7_P171_media.fcs · Exp1_MCF7_P171_EGF10min.fcs · Exp1_MCF7_P171_starve.fcs · Exp2_MCF7_P171_EGF10min.fcs · Exp2_MCF7_P171_starve.fcs · Exp3_MCF7_media.fcs |
| · MDAMB231 | Exp1_MDAMB231_P62_EGF10min.fcs · Exp1_MDAMB231_P62_media.fcs · Exp1_MDAMB231_P62_starve.fcs · Exp2_MDAMB231_P62_EGF10min.fcs · Exp2_MDAMB231_P62_starve.fcs · Exp3_MDAMB231_media.fcs |
| · SKBR3 | Exp1_SKBR3_P45_EGF10min.fcs · Exp1_SKBR3_P45_media.fcs · Exp1_SKBR3_P45_starve.fcs · Exp2_SKBR3_P45_EGF10min.fcs · Exp2_SKBR3_P45_starve.fcs · Exp3_SKBR3_media.fcs |
| · SUM149 | Exp1_SUM149_P37_EGF10min.fcs · Exp1_SUM149_P37_media.fcs · Exp1_SUM149_P37_starve.fcs · Exp2_SUM149_P37_EGF10min.fcs · Exp2_SUM149_P37_starve.fcs · Exp3_SUM149_media.fcs |
| · T47D | Exp1_T47D_P89_EGF10min.fcs · Exp1_T47D_P89_media.fcs · Exp1_T47D_P89_starve.fcs · Exp2_T47D_P89_EGF10min.fcs · Exp2_T47D_P89_starve.fcs · Exp3_T47D_media.fcs |
| · ZR75-1 | Exp1_19_ZR75-1_P102_media.fcs · Exp1_ZR75-1_P102_EGF10min.fcs · Exp1_ZR75-1_P102_starve.fcs · Exp2_ZR75-1_P102_EGF10min.fcs · Exp2_ZR75-1_P102_starve.fcs · Exp3_ZR75-1_media.fcs |
