Experiment Overview
| Repository ID: | FR-FCM-ZYBN | Experiment name: | CD5–NK1.1+ γδ T cells that develop in a Bcl11b-independent manner participate in early protection against infection | MIFlowCyt score: | 43.67% |
| Primary researcher: | Yasunbou Yoshikai | PI/manager: | Yasunbou Yoshikai | Uploaded by: | Yasunbou Yoshikai |
| Experiment dates: | 2014-02-05 - 2017-08-08 | Dataset uploaded: | Sep 2017 | Last updated: | Dec 2017 |
| Keywords: | [Bacteria] [IL-17A] [IFN-γ] [innate immunity] [γδ T cell] [Bcl11b] [DN2a] [Granzyme] [Host defense] [Listeria monocytogenes] | Manuscripts: | [29091759] | ||
| Organizations: | None | ||||
| Purpose: | Among the innate-like γδ T cells in the fetal thymus committed to forming IFN-γ-producing effector cells, those programmed to develop at the earlier DN2-stage in a Bcl11b-independent manner seem to be more primitive T cells of the innate immune system. The ontogenetic wave of γδ T cell development in the thymus suggests that Bcl11b-independent γδ T cells play a critical role in protecting against infections at the earlier stages after infection. To test this hypothesis, we characterized the innate-like γδ T cells that developed from the DN2a-stage in a Bcl11b-independent manner using Bcl11b conditionally deleted mice, in which T cell development is completely blocked before the DP stage. | ||||
| Conclusion: | Bcl11b-independent γδ T cells had a CD5– NK1.1+ Granzyme+ phenotype and were abundant in the liver in WT mice. Bcl11b-independent γδ T cells contributed to early protection against L. monocytogenes infection. Bcl11b-independent γδ T cells participate in early protection as “primitive innate-like γδ T cells” in host defense. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
