Experiment Overview
| Repository ID: | FR-FCM-ZYBP | Experiment name: | OMIP-043: Identification of Human Antibody Secreting subsets | MIFlowCyt score: | 67.75% |
| Primary researcher: | Jeff Carrell | PI/manager: | Jeff Carrell | Uploaded by: | Jeff Carrell |
| Experiment dates: | 2014-03-06 - 2017-09-08 | Dataset uploaded: | Sep 2017 | Last updated: | Aug 2018 |
| Keywords: | [flow cytometry] [OMIP] [plasma cells] | Manuscripts: | [29286577] |
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| Organizations: |
MedImmune, LLC, Gaithersburg, MD (United States)
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| Purpose: | The purpose of our OMIP manuscript is to describe a standardized means of identifying antibody-secreting cells in a variety of human tissues, for their quantification and immunoglobulin distribution. | ||||
| Conclusion: | We believe that our work will serve as a useful reference for those that are studying rare antibody-secreting cells in health and disease. In light of recent publications that present evolving evidence about ASC phenotype, their longevity and role in vaccine responses (Halliley 2015, Mei 2015), our work may help investigators to further understand these cells, how they circulate and provide immune surveillance. In development of this panel, we employed modern fluorophores, high-parameter instruments, and emphasized sound statistics when evaluating low-frequency cells. | ||||
| Comments: | As this is a prospective method, variables were limited to differences inherent between tissue types and donor variation. We did not introduce additional variable beyond the testing of various clone-fluor combinations. The method was developed over the course of many experiments from 2014-2017. To conform to rigorous MIFlowCyt standards, a concise data set was prepared in 2017 to encompass antibody titrations and a representative set of data from 4 human tissues (peripheral blood mononuclear cells, bone marrow, spleen, and tonsil). The 2017 data set was collected on a single cytometer over several weeks and will be submitted to the repository. | ||||
| Funding: | We are a publicly traded biopharm company. | ||||
| Quality control: | Our facility has a daily instrument QA/QC procedure consisting of a primary particle (Cytometer Setup and Tracking beads, or CST, BDBiosciences) used to assess consistency of day-to-day performance and establish laser delays, and a secondary particle (Spherotech 8-peak Rainbow particles) used to assess resolution in each cytometer channel. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Peripheral blood mononuclear cells | PBMC_C1_C01_005.fcs · PBMC_C2_C02_006.fcs · PBMC_C3_C03_007.fcs · PBMC_C4_C04_008.fcs · PBMC_C5_C05_009.fcs · PBMC_C6_C06_010.fcs · PBMC_C7_C07_011.fcs · PBMC_unst_A1_A01_001.fcs |
| · bone marrow cells | BM_D1_D01_012.fcs · BM_D2_D02_013.fcs · BM_D3_D03_014.fcs · BM_D4_D04_015.fcs · BM_D5_D05_016.fcs · BM_D6_D06_017.fcs · BM_D7_D07_018.fcs · BM_unst_A2_A02_002.fcs |
| · spleen cells | Spl_unst_A3_A03_003.fcs · Spleen_E1_E01_019.fcs · Spleen_E2_E02_020.fcs · Spleen_E3_E03_021.fcs · Spleen_E4_E04_022.fcs · Spleen_E5_E05_023.fcs · Spleen_E6_E06_024.fcs · Spleen_E7_E07_025.fcs |
| · tonsil cells | Tons_unst_A4_A04_004.fcs · Tonsil_F1_F01_026.fcs · Tonsil_F2_F02_027.fcs · Tonsil_F3_F03_028.fcs · Tonsil_F4_F04_029.fcs · Tonsil_F5_F05_030.fcs · Tonsil_F6_F06_031.fcs · Tonsil_F7_F07_032.fcs |
| · titration files | APC CD19_cct.fcs · BD APC CD19 CloneSJ2CL_A8_A08_057.fcs · BD APC CD19 CloneSJ2CL_B8_B08_058.fcs · BD APC CD19 CloneSJ2CL_C8_C08_059.fcs · BD APC CD19 CloneSJ2CL_D8_D08_060.fcs · BD APC CD19 CloneSJ2CL_E8_E08_061.fcs · BD APC CD19 CloneSJ2CL_F8_F08_062.fcs · BD APC CD19 CloneSJ2CL_G8_G08_063.fcs · BD APC CD19 CloneSJ2CL_H8_H08_064.fcs · BD BUV395 CD38 Clone HB7_A3_A03_017.fcs · BD BUV395 CD38 Clone HB7_B3_B03_018.fcs · BD BUV395 CD38 Clone HB7_C3_C03_019.fcs · BD BUV395 CD38 Clone HB7_D3_D03_020.fcs · BD BUV395 CD38 Clone HB7_E3_E03_021.fcs · BD BUV395 CD38 Clone HB7_F3_F03_022.fcs · BD BUV395 CD38 Clone HB7_G3_G03_023.fcs · BD BUV395 CD38 Clone HB7_H3_H03_024.fcs · BD BV510 CD14 Clone MOPG_A6_A06_041.fcs · BD BV510 CD14 Clone MOPG_B6_B06_042.fcs · BD BV510 CD14 Clone MOPG_C6_C06_043.fcs · BD BV510 CD14 Clone MOPG_D6_D06_044.fcs · BD BV510 CD14 Clone MOPG_E6_E06_045.fcs · BD BV510 CD14 Clone MOPG_F6_F06_046.fcs · BD BV510 CD14 Clone MOPG_G6_G06_047.fcs · BD BV510 CD14 Clone MOPG_H6_H06_048.fcs · BD BV510 CD15 CloneW6D3_A7_A07_049.fcs · BD BV510 CD15 CloneW6D3_B7_B07_050.fcs · BD BV510 CD15 CloneW6D3_C7_C07_051.fcs · BD BV510 CD15 CloneW6D3_D7_D07_052.fcs · BD BV510 CD15 CloneW6D3_E7_E07_053.fcs · BD BV510 CD15 CloneW6D3_F7_F07_054.fcs · BD BV510 CD15 CloneW6D3_G7_G07_055.fcs · BD BV510 CD15 CloneW6D3_H7_H07_056.fcs · BD BV510 CD3 Clone UCHT1_A5_A05_033.fcs · BD BV510 CD3 Clone UCHT1_B5_B05_034.fcs · BD BV510 CD3 Clone UCHT1_C5_C05_035.fcs · BD BV510 CD3 Clone UCHT1_D5_D05_036.fcs · BD BV510 CD3 Clone UCHT1_E5_E05_037.fcs · BD BV510 CD3 Clone UCHT1_F5_F05_038.fcs · BD BV510 CD3 Clone UCHT1_G5_G05_039.fcs · BD BV510 CD3 Clone UCHT1_H5_H05_040.fcs · BD PerCPCY55 CD IgD Clone1A6-2_A4_A04_025.fcs · BD PerCPCY55 CD IgD Clone1A6-2_B4_B04_026.fcs · BD PerCPCY55 CD IgD Clone1A6-2_C4_C04_027.fcs · BD PerCPCY55 CD IgD Clone1A6-2_D4_D04_028.fcs · BD PerCPCY55 CD IgD Clone1A6-2_E4_E04_029.fcs · BD PerCPCY55 CD IgD Clone1A6-2_F4_F04_030.fcs · BD PerCPCY55 CD IgD Clone1A6-2_G4_G04_031.fcs · BD PerCPCY55 CD IgD Clone1A6-2_H4_H04_032.fcs · BL BV421 CD20 Clone 2H7_A1_A01_001.fcs · BL BV421 CD20 Clone 2H7_B1_B01_002.fcs · BL BV421 CD20 Clone 2H7_C1_C01_003.fcs · BL BV421 CD20 Clone 2H7_D1_D01_004.fcs · BL BV421 CD20 Clone 2H7_E1_E01_005.fcs · BL BV421 CD20 Clone 2H7_F1_F01_006.fcs · BL BV421 CD20 Clone 2H7_G1_G01_007.fcs · BL BV421 CD20 Clone 2H7_H1_H01_008.fcs · BL BV785 CD27 Clone O323_A2_A02_009.fcs · BL BV785 CD27 Clone O323_B2_B02_010.fcs · BL BV785 CD27 Clone O323_C2_C02_011.fcs · BL BV785 CD27 Clone O323_D2_D02_012.fcs · BL BV785 CD27 Clone O323_E2_E02_013.fcs · BL BV785 CD27 Clone O323_F2_F02_014.fcs · BL BV785 CD27 Clone O323_G2_G02_015.fcs · BL BV785 CD27 Clone O323_H2_H02_016.fcs · BUV395 CD38_cct.fcs · BV421 CD20_cct.fcs · BV510 CD14_cct.fcs · BV510 CD15_cct.fcs · BV510 CD3_cct.fcs · BV785 CD27_cct.fcs · CD14_BV510a_A5_A05_044.fcs · CD14_BV510a_A5_A05_044_cct.fcs · CD14_BV510a_B5_B05_045.fcs · CD14_BV510a_C5_C05_046.fcs · CD14_BV510a_D5_D05_047.fcs · CD14_BV510a_E5_E05_048.fcs · CD14_BV510a_F5_F05_049.fcs · CD14_BV510a_G5_G05_050.fcs · CD14_BV510a_H5_H05_051.fcs · CD15_BV510_A7_A07_012.fcs · CD15_BV510_A7_A07_012_cct.fcs · CD15_BV510_B7_B07_013.fcs · CD15_BV510_C7_C07_014.fcs · CD15_BV510_D7_D07_015.fcs · CD15_BV510_E7_E07_016.fcs · CD15_BV510_F7_F07_017.fcs · CD15_BV510_G7_G07_018.fcs · CD15_BV510_H7_H07_019.fcs · CD19_APC_altA10_A10_036_cct.fcs · CD19_APC_alt_A10_A10_036.fcs · CD19_APC_alt_B10_B10_037.fcs · CD19_APC_alt_C10_C10_038.fcs · CD19_APC_alt_D10_D10_039.fcs · CD19_APC_alt_E10_E10_040.fcs · CD19_APC_alt_F10_F10_041.fcs · CD19_APC_alt_G10_G10_042.fcs · CD19_APC_alt_H10_H10_043.fcs · CD38_BUV395_A9_A09_028.fcs · CD38_BUV395_A9_A09_028_cct.fcs · CD38_BUV395_B9_B09_029.fcs · CD38_BUV395_C9_C09_030.fcs · CD38_BUV395_D9_D09_031.fcs · CD38_BUV395_E9_E09_032.fcs · CD38_BUV395_F9_F09_033.fcs · CD38_BUV395_G9_G09_034.fcs · CD38_BUV395_H9_H09_035.fcs · CD38_PECy7_A8_A08_020.fcs · CD38_PECy7_A8_A08_020_cct.fcs · CD38_PECy7_B8_B08_021.fcs · CD38_PECy7_C8_C08_022.fcs · CD38_PECy7_D8_D08_023.fcs · CD38_PECy7_E8_E08_024.fcs · CD38_PECy7_F8_F08_025.fcs · CD38_PECy7_G8_G08_026.fcs · CD38_PECy7_H8_H08_027.fcs · CELLS ONLY_A9_A09_065.fcs · CELLS ONLY_B9_B09_066.fcs · CELLS ONLY_C9_C09_067.fcs · CELLS ONLY_D9_D09_068.fcs · DAPI ONLY_E9_E09_069.fcs · DAPI ONLY_F9_F09_070.fcs · DAPI ONLY_G9_G09_071.fcs · DAPI ONLY_H9_H09_072.fcs · IgA_mAb_FITC_A7_A07_026.fcs · IgA_mAb_FITC_B7_B07_027.fcs · IgA_mAb_FITC_C7_C07_028.fcs · IgA_mAb_FITC_D7_D07_029.fcs · IgA_mAb_FITC_E7_E07_030.fcs · IgA_mAb_FITC_F7_F07_031.fcs · IgA_mAb_FITC_G7_G07_032.fcs · IgA_mAb_FITC_H7_H07_033.fcs · IgA_mAb_cct.fcs · IgA_pAb_FITC_A6_A06_018.fcs · IgA_pAb_FITC_B6_B06_019.fcs · IgA_pAb_FITC_C6_C06_020.fcs · IgA_pAb_FITC_D6_D06_021.fcs · IgA_pAb_FITC_E6_E06_022.fcs · IgA_pAb_FITC_F6_F06_023.fcs · IgA_pAb_FITC_G6_G06_024.fcs · IgA_pAb_FITC_H6_H06_025.fcs · IgA_pAb_cct.fcs · IgG_BV605_A8_A08_034.fcs · IgG_BV605_B8_B08_035.fcs · IgG_BV605_C8_C08_036.fcs · IgG_BV605_D8_D08_037.fcs · IgG_BV605_E8_E08_038.fcs · IgG_BV605_F8_F08_039.fcs · IgG_BV605_G8_G08_040.fcs · IgG_BV605_H8_H08_041.fcs · IgG_BV605_cct.fcs · IgG_FITC_A4_A04_002.fcs · IgG_FITC_B4_B04_003.fcs · IgG_FITC_C4_C04_004.fcs · IgG_FITC_D4_D04_005.fcs · IgG_FITC_E4_E04_006.fcs · IgG_FITC_F4_F04_007.fcs · IgG_FITC_G4_G04_008.fcs · IgG_FITC_H4_H04_009.fcs · IgG_FITC_cct.fcs · IgG_FITC_cctNEW.fcs · IgG_FITC_cctNEWest.fcs · IgM_BV605_A9_A09_042.fcs · IgM_BV605_B9_B09_043.fcs · IgM_BV605_C9_C09_044.fcs · IgM_BV605_D9_D09_045.fcs · IgM_BV605_E9_E09_046.fcs · IgM_BV605_F9_F09_047.fcs · IgM_BV605_G9_G09_048.fcs · IgM_BV605_H9_H09_049.fcs · IgM_BV605_cct.fcs · IgM_FITC_A5_A05_010.fcs · IgM_FITC_B5_B05_011.fcs · IgM_FITC_C5_C05_012.fcs · IgM_FITC_D5_D05_013.fcs · IgM_FITC_E5_E05_014.fcs · IgM_FITC_F5_F05_015.fcs · IgM_FITC_G5_G05_016.fcs · IgM_FITC_H5_H05_017.fcs · IgM_FITC_cct.fcs · IgM_FITC_cctNEWEST.fcs · Ki67_PE_A10_A10_050.fcs · Ki67_PE_B10_B10_051.fcs · Ki67_PE_C10_C10_052.fcs · Ki67_PE_D10_D10_053.fcs · Ki67_PE_E10_E10_054.fcs · Ki67_PE_F10_F10_055.fcs · Ki67_PE_G10_G10_056.fcs · Ki67_PE_H10_H10_057.fcs · Ki67_PE_cct.fcs · LWB_setup_A1_A01_001.fcs · PerCPCY55 IgD_cct.fcs · unst_Tons_A1_A01_001.fcs |
