Experiment Overview
| Repository ID: | FR-FCM-ZYD2 | Experiment name: | Flow cytometric analysis of thymic epithelial cells from fetal thymic organ cultures | MIFlowCyt score: | 52.72% |
| Primary researcher: | Charles St-Pierre | PI/manager: | Charles St-Pierre | Uploaded by: | Charles St-Pierre |
| Experiment dates: | 2015-03-09 - 2017-08-31 | Dataset uploaded: | Oct 2017 | Last updated: | Oct 2017 |
| Keywords: | [Thymus] [thymic epithelial cells] [thymic organ cultures] | Manuscripts: | [29186691] |
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| Organizations: |
Institute for Research in Immunology and Cancer, University of Montreal / Department of Medicine, Montreal, Quebec (Canada)
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| Purpose: | Evaluate the abundance and proportion of thymic epithelial cells in fetal thymic organ cultures derived from embryonic day 14.5 thymic lobes | ||||
| Conclusion: | None | ||||
| Comments: | Thymic lobes were isolated from E14.5 C57BL/6 (B6) embryos and cultured on Isopore membrane filters (Millipore) placed on Gelfoam sponge (Pfizer) floated in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum, 50 mM 2-mercaptoethanol, 2 mM L-glutamine, 100 U ml-1 penicillin / streptomycin (Life Technologies). For depletion of thymocytes, thymic lobes were cultured for 5 days with 1.35 mM 2’-deoxyguanosine (SIGMA). Thymic lobes were disaggregated with 0.1 % DNase I (Sigma) and 0.01% Liberase (Roche) prior to flow cytometry analysis. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
