The FlowRepository admin email (flowrepository-admin@isac-net.org) has not been working since December 13th 2018 and is still not working. From January 3rd 2019 the website will now link directly to the admin, Justin, through his email of justinmeskas@gmail.com. If you had sent a support ticket or any other means to connect to the admins of FlowRepository between those two dates, those emails did not go through. Please forward any emails to him, or re-create any issues through the website. Sorry for any inconvenience and thank you for your patience and understanding.

Download-button_s

Experiment Overview

Repository ID: FR-FCM-ZYFB Experiment name: OMIP-047: High-dimensional phenotypic characterization of B cells MIFlowCyt score: 84.00%
Primary researcher: Thomas Liechti PI/manager: Thomas Liechti Uploaded by: Thomas Liechti
Experiment dates: 2014-03-31 - 2015-02-28 Dataset uploaded: Dec 2017 Last updated: Aug 2018
Keywords: [B cells] [chemokine receptors] [plasmablasts] [cytokine receptors] [surface immunoglobulin isotypes] [chronic viral diseases ] Manuscripts: [29782066] Cytalogo
Organizations: University of Zurich, Institute of Medical Virology, Zürich, (Switzerland)
Purpose: Study the dynamics of B cell subsets and their phenotype in chronic viral diseases
Conclusion: This panel allows the identification of all major B cell subsets. The phenotypic profile encompassing chemokine receptors, immunoglobulin isotypes, IL-21R and the proliferation marker Ki67 show sufficient resolution in order to determine the phenotypic characteristics of B cell subsets.
Comments: None
Funding: Not disclosed
Quality control: Quality control of the BD Fortessa flow cytometer was regularly performed using the BD CS&T module. Antibodies were titrated to determine the optimal staining index and if necessary antibody concentration was adjusted to reduce spillover-dependent spreading. FMO controls were used to determine expression of dimly expressed markers.


Experiment variables

Conditions
· FMO controls 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs
· healthy donor 150416_HC13_037.fcs

Sample Type
· Healthy donor 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs

Individuals
· Healthy donors 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs

Download FCS Files or login and see the dataset in your inbox for further annotation details.