Experiment Overview
| Repository ID: | FR-FCM-ZYFB | Experiment name: | OMIP-047: High-dimensional phenotypic characterization of B cells | MIFlowCyt score: | 84.00% |
| Primary researcher: | Thomas Liechti | PI/manager: | Thomas Liechti | Uploaded by: | Thomas Liechti |
| Experiment dates: | 2014-03-31 - 2015-02-28 | Dataset uploaded: | Dec 2017 | Last updated: | Aug 2018 |
| Keywords: | [B cells] [chemokine receptors] [plasmablasts] [cytokine receptors] [surface immunoglobulin isotypes] [chronic viral diseases ] | Manuscripts: | [29782066] |
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| Organizations: |
University of Zurich, Institute of Medical Virology, Zürich, (Switzerland)
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| Purpose: | Study the dynamics of B cell subsets and their phenotype in chronic viral diseases | ||||
| Conclusion: | This panel allows the identification of all major B cell subsets. The phenotypic profile encompassing chemokine receptors, immunoglobulin isotypes, IL-21R and the proliferation marker Ki67 show sufficient resolution in order to determine the phenotypic characteristics of B cell subsets. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Quality control of the BD Fortessa flow cytometer was regularly performed using the BD CS&T module. Antibodies were titrated to determine the optimal staining index and if necessary antibody concentration was adjusted to reduce spillover-dependent spreading. FMO controls were used to determine expression of dimly expressed markers. | ||||
Experiment variables
| Conditions | |
|---|---|
| · FMO controls | 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs |
| · HIV+ | 150120_AK156 070309 late chronic_031.fcs |
| · healthy donor | 150416_HC13_037.fcs |
| Sample Type | |
|---|---|
| · Healthy donor | 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs |
| · HIV+ | 150120_AK156 070309 late chronic_031.fcs |
| Individuals | |
|---|---|
| · Healthy donors | 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs |
| · HIV+ | 150120_AK156 070309 late chronic_031.fcs |
