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Experiment Overview

Repository ID: FR-FCM-ZYFB Experiment name: OMIP-047: High-dimensional phenotypic characterization of B cells MIFlowCyt score: 84.00%
Primary researcher: Thomas Liechti PI/manager: Thomas Liechti Uploaded by: Thomas Liechti
Experiment dates: 2014-03-31 - 2015-02-28 Dataset uploaded: Dec 2017 Last updated: Aug 2018
Keywords: [B cells] [chemokine receptors] [plasmablasts] [cytokine receptors] [surface immunoglobulin isotypes] [chronic viral diseases ] Manuscripts: [29782066] Cytalogo
Organizations: University of Zurich, Institute of Medical Virology, Zürich, (Switzerland)
Purpose: Study the dynamics of B cell subsets and their phenotype in chronic viral diseases
Conclusion: This panel allows the identification of all major B cell subsets. The phenotypic profile encompassing chemokine receptors, immunoglobulin isotypes, IL-21R and the proliferation marker Ki67 show sufficient resolution in order to determine the phenotypic characteristics of B cell subsets.
Comments: None
Funding: Not disclosed
Quality control: Quality control of the BD Fortessa flow cytometer was regularly performed using the BD CS&T module. Antibodies were titrated to determine the optimal staining index and if necessary antibody concentration was adjusted to reduce spillover-dependent spreading. FMO controls were used to determine expression of dimly expressed markers.


Experiment variables

Conditions
· FMO controls 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs
· healthy donor 150416_HC13_037.fcs

Sample Type
· Healthy donor 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs

Individuals
· Healthy donors 150416_FMO CXCR3_HC13_021.fcs · 150416_FMO CXCR4_HC13_022.fcs · 150416_FMO CXCR5_HC13_023.fcs · 150416_FMO IL-21R_HC13_024.fcs · 150416_FMO KI67_HC13_025.fcs · 150416_HC13_037.fcs
· HIV+ 150120_AK156 070309 late chronic_031.fcs

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