Experiment Overview
| Repository ID: | FR-FCM-ZYFV | Experiment name: | Knapp Hammond et al Human CD34+ Cord Blood | MIFlowCyt score: | 68.00% |
| Primary researcher: | David JHF Knapp | PI/manager: | David JHF Knapp | Uploaded by: | David JHF Knapp |
| Experiment dates: | 2015-05-03 - 2015-07-03 | Dataset uploaded: | Jan 2018 | Last updated: | Apr 2018 |
| Keywords: | [hematopoietic stem cells] [mass cytometry] [CyTOF] [HSC] [Progenitor Cells ] [Human cord blood] [CD34+] [haematopoiesis] [CD49f cells] [haematopoietic stem cell] | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
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| Purpose: | To investigate the molecular diversity of human CD34+ umbilical cord blood, to compare phenotypic and functionally defined subsets, and to identify molecular parameters which may be involved in the regulation of cell functional behaviour. | ||||
| Conclusion: | Classical phenotypic subsets contain mixtures of cell types as defined by both molecular characteristics and function. Functional populations within the CD34 fraction show a generally continuous distribution in molecular space rather than showing completely discrete characteristics. | ||||
| Comments: | None | ||||
| Funding: | This work was supported by a Terry Fox Foundation New Frontiers Program Project, and a Stem Cell Network of Centres of Excellence grant. DJHFK held a Canadian Institutes of Health Research (CIHR) Vanier Scholarship. PHM held a CIHR Frederick Banting and Charles Best Doctoral Scholarship. DVS sciences provided the Palladium barcoding reagents used in the mass cytometry experiments. | ||||
| Quality control: | 4EQ beads were included in run to allow intensity normalization. All samples were collected, fixed, and stored until time of run at which point all were palladium barcoded to allow staining in a single tube, and thus equalizing antibody exposure across samples. | ||||
Experiment variables
| Individuals | |
|---|---|
| · Sample 1 | Sample 1.fcs |
| · Sample 2 | Sample 2.fcs |
| · Sample 3 | Sample 3.fcs |
| · Sample 4 | Sample 4.fcs |
| · Sample 5 | Sample 5.fcs |
| · Sample 6 | Sample 6.fcs |
| · Sample 7 | Sample 7.fcs |
| · Sample 8 | Sample 8.fcs |
| Conditions | |
|---|---|
| · Cryopreserved | Sample 1.fcs · Sample 2.fcs · Sample 3.fcs · Sample 4.fcs · Sample 5.fcs |
| · Fresh | Sample 6.fcs · Sample 7.fcs · Sample 8.fcs |
