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Conclusion:
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We found that CD32a+ CD4+ T cells include heterogeneous subsets that are differentially affected by HIV infection. Our analysis revealed that Naive (N), central memory (CM), and effector/memory (Eff/Mem) CD32a+ CD4+ T-cell clusters that co-express LILRA2 and CD64 activating receptors were more abundant in primary HIV infection and cART stages. Conversely, LILRA2- CD32a+ CD4+ T-cell clusters of either the TN, TCM, or TEff/Mem phenotype were more abundant in healthy individuals. Finally, an activated CD32a+ CD4+ TEff/Mem cell cluster co-expressing LILRA2, CD57, and NKG2C was more abundant in all HIV stages, particularly during primary HIV infection. This cell cluster positively correlated with the amount of HIV DNA. Overall, our data show that multiple modifications in CD32a+ CD4+ T-cell subsets occur in the early phase of HIV infection, and some of which are conserved after effective cART. Such analysis provides new insights into the heterogeneity and dynamics of CD32a+ CD4+ T-cell populations that may help to better identify and reduce viral reservoirs.
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