Experiment Overview
| Repository ID: | FR-FCM-ZYGW | Experiment name: | Investigation of the membrane integrity of mycobacteria | MIFlowCyt score: | 52.63% |
| Primary researcher: | CARINE SAO | PI/manager: | CARINE SAO | Uploaded by: | CARINE SAO |
| Experiment dates: | 2017-02-01 - 2018-02-01 | Dataset uploaded: | Feb 2018 | Last updated: | Feb 2018 |
| Keywords: | [ergothioneine] [mycothiol] [gamma-glutamylcysteine] [mycobacterial membrane] [thiols secretion] | Manuscripts: | [29437626] |
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| Organizations: |
Stellenbosch University, Biomedical Sciences, Cape Town, Western Cape (South Africa)
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| Purpose: | Demonstrate that the membrane of some Mycobacterium tuberculosis mutants is not more damaged than the membrane of the wild type. Demonstrate that the detected extracellular thiols could be as a result of a secretion. | ||||
| Conclusion: | The membrane of tested thiol-defcient mutants is not more damaged than that of the wild type strain. Therefore the elevated extracellular thiols could result from a secretion. | ||||
| Comments: | The deficiency of one thiol results leads to an increased intracellular production and extracellular secretion of the other thiols in Mycobacterium tuberculosis. | ||||
| Funding: | The South African Medical Research Council (SAMRC), the DST-NRF Centre of Excellence in Biomedical Tuberculosis Research, the Harry Crossley Foundation and Norvatis (Basel, Switzerland). | ||||
| Quality control: | Samples were washed twice and re-suspended in 150 mM NaCl. | ||||
