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Experiment Overview

Repository ID: FR-FCM-ZYGY Experiment name: OMIP: Maturation and Polarization Markers of Human Myeloid Subsets Suitable for Myelopoiesis Study or Differentiation of Myeloid Neoplasms MIFlowCyt score: 82.00%
Primary researcher: Adam Mailloux PI/manager: Adam Mailloux Uploaded by: Adam Mailloux
Experiment dates: 2016-09-01 - 2017-09-01 Dataset uploaded: Jan 2018 Last updated: Feb 2019
Keywords: [Acute Myeloid Leukemia] [myelodysplastic syndrome] [myeloid neoplasms] [myeloproliferative neoplasm] [bone marrow hematopoiesis] Manuscripts: [30347519] Cytalogo
Organizations: H Lee Moffitt Cancer Center, H Lee Moffitt Cancer Center, Tampa, FL (United States)
Purpose: Maturation and Polarization Markers of Human Myeloid Subsets Suitable for Myelopoiesis Study or Differentiation of Myeloid Neoplasms
Conclusion: An optimized panel for the analysis of hematopoietic cells or myeloid neoplasms
Comments: File include compensation controls, fluorescence minus one (FMO) controls, "build" fcs files in which groups of antibodies are successively included to construct the panel, one healthy bone marrow mononuclear cell control, and two de-identified CMLL patient samples
Funding: Moffitt Cancer Center – Innovative Core Projects (Project number 16060201)
Quality control: compensation controls, fluorescence minus one (FMO) controls, and one healthy bone marrow mononuclear cell control


Experiment variables

Sample Type
· FMO Controls Specimen_001_FMO AF700_036.fcs · Specimen_001_FMO APC-Cy7_024.fcs · Specimen_001_FMO APC_038.fcs · Specimen_001_FMO BB515_026.fcs · Specimen_001_FMO BUV395_035.fcs · Specimen_001_FMO BUV496_030.fcs · Specimen_001_FMO BUV737_034.fcs · Specimen_001_FMO BUV805_025.fcs · Specimen_001_FMO BV421_031.fcs · Specimen_001_FMO BV605_037.fcs · Specimen_001_FMO BV650_027.fcs · Specimen_001_FMO BV711_040.fcs · Specimen_001_FMO BV786_029.fcs · Specimen_001_FMO LD Aqua BV510_023.fcs · Specimen_001_FMO PE-CF594_028.fcs · Specimen_001_FMO PE-CY7_032.fcs · Specimen_001_FMO PE_039.fcs · Specimen_001_FMO PerCP-Cy55_033.fcs
· Healthy BMMNC Specimen_001_BMMNC_003.fcs
· CMML BMMNC Specimen_001_2 V 005_006.fcs · Specimen_001_3 E 005_005.fcs
· Panel build files Specimen_001_Part 1_042.fcs · Specimen_001_Part 2_021.fcs · Specimen_001_Part 3_022.fcs · Specimen_001_Part 4_020.fcs

Conditions
· FMO Controls Specimen_001_FMO AF700_036.fcs · Specimen_001_FMO APC-Cy7_024.fcs · Specimen_001_FMO APC_038.fcs · Specimen_001_FMO BB515_026.fcs · Specimen_001_FMO BUV395_035.fcs · Specimen_001_FMO BUV496_030.fcs · Specimen_001_FMO BUV737_034.fcs · Specimen_001_FMO BUV805_025.fcs · Specimen_001_FMO BV421_031.fcs · Specimen_001_FMO BV605_037.fcs · Specimen_001_FMO BV650_027.fcs · Specimen_001_FMO BV711_040.fcs · Specimen_001_FMO BV786_029.fcs · Specimen_001_FMO LD Aqua BV510_023.fcs · Specimen_001_FMO PE-CF594_028.fcs · Specimen_001_FMO PE-CY7_032.fcs · Specimen_001_FMO PE_039.fcs · Specimen_001_FMO PerCP-Cy55_033.fcs
· Healthy BMMNC Specimen_001_BMMNC_003.fcs
· CMML BMMNC Specimen_001_2 V 005_006.fcs · Specimen_001_3 E 005_005.fcs
· Panel build files Specimen_001_Part 1_042.fcs · Specimen_001_Part 2_021.fcs · Specimen_001_Part 3_022.fcs · Specimen_001_Part 4_020.fcs

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