Experiment Overview
| Repository ID: | FR-FCM-ZYHL | Experiment name: | FCM data input modes - Murine skin-draining lymph-node CD45 - mDCs | MIFlowCyt score: | 50.75% |
| Primary researcher: | Daniel Jimenez-Carretero | PI/manager: | Daniel Jimenez-Carretero | Uploaded by: | Daniel Jimenez-Carretero |
| Experiment dates: | 2018-01-23 - 2018-02-28 | Dataset uploaded: | Feb 2018 | Last updated: | Jul 2018 |
| Keywords: | [flow cytometry] [dimension reduction] [Compensation] [high-dimensional cytometry] [automated data analysis] [automated phenotyping] [unsupervised clustering] [data preparation] [spectral data] [pseudo-spectral data] [data-input modes evaluation] | Manuscripts: | [29735643] | ||
| Organizations: |
Spanish National Center for Cardiovascular Research (CNIC), Cellomics Unit, Madrid, Madrid (Spain)
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| Purpose: | Multi-input fcs files merging the different data input modes for conventional and spectral flow cytometers | ||||
| Conclusion: | Automated phenotypic analysis can be improved by configuring instrument acquisition to use all available fluorescence detectors, and avoiding integration and compensation procedures, thereby using raw spectral or pseudospectral data. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Conventional Cytometry | CONV_DC_CD45_KO1.fcs · CONV_DC_CD45_KO2.fcs · CONV_DC_CD45_KO3.fcs · CONV_DC_CD45_KO4.fcs · CONV_DC_CD45_KO5.fcs · CONV_DC_CD45_WT1.fcs · CONV_DC_CD45_WT2.fcs · CONV_DC_CD45_WT3.fcs · CONV_DC_CD45_WT4.fcs · CONV_DC_CD45_WT5.fcs |
| · Spectral Cytometry | SPC_DC_CD45_KO1.fcs · SPC_DC_CD45_KO2.fcs · SPC_DC_CD45_KO3.fcs · SPC_DC_CD45_KO4.fcs · SPC_DC_CD45_KO5.fcs · SPC_DC_CD45_WT1.fcs · SPC_DC_CD45_WT2.fcs · SPC_DC_CD45_WT3.fcs · SPC_DC_CD45_WT4.fcs · SPC_DC_CD45_WT5.fcs |
