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Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
We apologize for the inconvenience and appreciate your understanding as we work on upgrading our hardware and improving the overall solution.
Thank you for your patience.

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Experiment Overview

Repository ID: FR-FCM-ZYLF Experiment name: Effect background fluorescence and spreading error in high-dimensional flow cytometry data visualization by tSNE MIFlowCyt score: 22.84%
Primary researcher: Jolanda Brummelman PI/manager: Enrico Lugli Uploaded by: Jolanda Brummelman
Experiment dates: 2017-04-04 - Dataset uploaded: Jun 2018 Last updated: Jun 2018
Keywords: [T cell] [CD8] [tSNE] [polychromatic flow cytometry] [single cell] [high-dimensional data] Manuscripts:
Organizations: None
Purpose: Testing the applicability of tSNE to 27-parameter single-cell flow cytometry data.
Conclusion: Background fluorescence and spreading error impact the visualisation of multidimensional single-cell data by tSNE substantially, specifically resulting in the generation of virtual “islands” that do not reflect real heterogeneity at the phenotypic or biological level.
Comments: None
Funding: Not disclosed
Quality control: None
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