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Experiment Overview

Repository ID: FR-FCM-ZYND Experiment name: Surface Marker Flow Cytometry in human eosinophils after glucocorticoid exposure MIFlowCyt score: 100.00%
Primary researcher: Michelle Makiya PI/manager: Luis Franco Uploaded by: Michelle Makiya
Experiment dates: 2017-12-11 - 2017-12-14 Dataset uploaded: Jul 2018 Last updated: Jul 2018
Keywords: [Eosinophils] [glucocorticoids] [CXCR4] [CCR1] [CCR3] Manuscripts: [29885264] Scientificdatanaturelogo
Organizations: National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases, Bethesda, MD (USA)
Purpose: Verify transcriptional changes from methylprednisolone treatment. After in vitro culture of peripheral blood leukocytes +/- methylprednisolone determine changes in surface expression of CCR1, CCR3 and CXCR4.
Conclusion: CXCR4 expression is upregulated in eosinophils upon methylprednisolone treatment of peripheral blood leukocytes.
Comments: None
Funding: This research was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases (NIAID) at the National Institutes of Health.
Quality control: 6 Normal Donor samples were run with identical conditions, treatments and time points, single color controls of each fluorochrome were run for compensation and FMO controls were run for gating


Experiment variables

Doses
· 20 ug/dL Methylprednisolone Patient_12_120min_MP20.fcs · Patient_13_120min_MP20.fcs · Patient_14_120min_MP20.fcs · Patient_15_120min_MP20.fcs · Patient_16_120min_MP20.fcs · Patient_17_120min_MP20.fcs
· 200 ug/dL Methylprednisolone Patient_12_120min_MP200.fcs · Patient_13_120min_MP200.fcs · Patient_14_120min_MP200.fcs · Patient_15_120min_MP200.fcs · Patient_16_120min_MP200.fcs · Patient_17_120min_MP200.fcs
· Vehicle (0.1% ethanol) Patient_12_120min_VH.fcs · Patient_13_120min_VH.fcs · Patient_14_120min_VH.fcs · Patient_15_120min_VH.fcs · Patient_16_120min_VH.fcs · Patient_17_120min_VH.fcs
· No treatment Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs

Timepoints
· Baseline Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs
· 120 minute Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs

Individuals
· Patient_12 Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs
· Patient_13 Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs
· Patient_14 Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs
· Patient_15 Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs
· Patient_16 Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs
· Patient_17 Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs

Conditions
· Unstained Patient_12_Unstained.fcs · Patient_13_Unstained.fcs · Patient_14_Unstained.fcs · Patient_15_Unstained.fcs · Patient_16_Unstained.fcs · Patient_17_Unstained.fcs
· Stained Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_12_Baseline_None.fcs · Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_13_Baseline_None.fcs · Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_14_Baseline_None.fcs · Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_15_Baseline_None.fcs · Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_16_Baseline_None.fcs · Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs · Patient_17_Baseline_None.fcs

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