Experiment Overview
| Repository ID: | FR-FCM-ZYNK | Experiment name: | Experiment_20160418 | MIFlowCyt score: | 27.00% |
| Primary researcher: | Iuliia Golovynska | PI/manager: | Iuliia Golovynska | Uploaded by: | Iuliia Golovynska |
| Experiment dates: | 2016-09-12 - 2017-10-25 | Dataset uploaded: | Jul 2018 | Last updated: | Feb 2019 |
| Keywords: | [flow cytometry] [Cisplatin] [Multipotent mesenchymal stromal cell] [mesenchymal-epithelial transition] [epidermal growth factor] [Toll-like receptor] [c-Myc] [teichoic acid] [Herceptin] [immunocytochemistry] | Manuscripts: | [30240134] |
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| Organizations: | None | ||||
| Purpose: | This study is aimed to reveal morphological and functional changes during mesenchymal-epithelial transition of multipotent mesenchymal stromal cells (MSCs) isolated from the rat bone marrow after: (i) activation of Toll-like receptors (TLR) with teichoic acid (TA), (ii) impact on epidermal growth factor (EGF) receptors with activator EGF or inhibitor Herceptin, and (iii) treatment with DNA intercalator Cisplatin. | ||||
| Conclusion: | The activation of EGFRs in MSCs isolated from the rat bone marrow induces MET, which is of high importance for regenerative medicine and therapy. Despite the ambiguous role of TLR signaling in the development of cells, no signs of the MSC transition to the epithelial phenotype were observed after the activation of these receptors. According to our results, EGF and TA cause an intensification of the synthesis of c-Myc and GAG content in the MSC cytoplasm, exhibiting indirect metabolic signs of the regenerative potential increase. In other experiments, inhibition of HER2-EGFR by Herceptin only suppressed the stemness signs of MSCs, confirming cytostatic effect of this known drug. Treatment of MSC with DNA intercalator Cisplatin was shown to seriously affect cell viability in general, reducing synthetic and proliferative activities and causing cell morphology disturbances. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Employment of high-quality techniques as flow cytometry, immunohistochemistry, optical imaging and multiple repetition of experiments for calculations | ||||
