Experiment Overview
| Repository ID: | FR-FCM-ZYQ9 | Experiment name: | Immune Subsets in Healthy Donor Bone Marrow | MIFlowCyt score: | 76.13% |
| Primary researcher: | Meghali Goswami | PI/manager: | Meghali Goswami | Uploaded by: | Meghali Goswami |
| Experiment dates: | 2017-02-09 - 2017-03-17 | Dataset uploaded: | Sep 2018 | Last updated: | Oct 2020 |
| Keywords: | [B cells] [flow cytometry] [NK cells] [monocytes] [healthy controls] [T cells] [dendritic cells] [bone marrow] [bone marrow mononuclear cells] | Manuscripts: | [30518681] [PMC6328018] | ||
| Organizations: |
National Heart, Lung, and Blood Institute (NHLBI), NIH, Hematology Branch, Bethesda, MD (USA)
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| Purpose: | To perform a comprehensive assessment of immune cell subsets in bone marrow from a cohort of adult human healthy donors with a large age range. https://www.biorxiv.org/content/early/2018/09/13/416750 | ||||
| Conclusion: | This resource provides a reference dataset for immune cell populations in healthy human bone marrow across a wide age range. Single cell RNA-sequencing quantification of marrow-resident cell populations has good concordance with immunophenotyping by flow and mass cytometry with some discrepancies in T and NK subsets. | ||||
| Comments: | https://pubmed.ncbi.nlm.nih.gov/30518681/ | ||||
| Funding: | This work was supported by the Intramural Research Program of the National Heart, Lung, and Blood Institute of the National Institutes of Health. | ||||
| Quality control: | Daily quality assurance was performed using Cytometer setup and Tracking beads (BD) as per manufacturer’s recommendation along with 1peak rainbow Beads (BD) and 8 peak beads (Spherotec)(Perfetto et al., 2012; Wang and Hoffman, 2017). | ||||
