Experiment Overview
| Repository ID: | FR-FCM-ZYQP | Experiment name: | Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry | MIFlowCyt score: | 77.50% |
| Primary researcher: | Andrew Reichard | PI/manager: | Kewal Asosingh | Uploaded by: | Andrew Reichard |
| Experiment dates: | 2018-09-24 - 2018-09-24 | Dataset uploaded: | Sep 2018 | Last updated: | Dec 2018 |
| Keywords: | [flow cytometry] [single cell suspension] [tissue digestion] [disaggregation] | Manuscripts: | [30523671] |
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| Organizations: |
Cleveland Clinic Lerner Research Institute, Inflammation and Immunity, Cleveland, Ohio (United States)
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| Purpose: | The purpose of the experiment was to provide comparative data to show quantitatively how improper techniques adversely affect the preparation of a single cell suspension from solid tissues for flow cytometry. | ||||
| Conclusion: | Freezing tissue, using a tissue homogenizer, vortexing cells, and over or under digesting tissues when preparing a single cell suspension resulted in decreased cell yield and viability and increased cell debris and fragments when compared to best practice procedures. | ||||
| Comments: | None | ||||
| Funding: | Supported by NIH grants HL103453, HL081064, and HL109250 | ||||
| Quality control: | CS&T Beads (BD Biosciences, San Jose, CA) were used to determine optimal PMT voltages and standardize the settings in FACSDiva 8.0.1. | ||||
