Experiment Overview
| Repository ID: | FR-FCM-ZYQV | Experiment name: | SOM3B Analysis of fresh healthy human bone marrow | MIFlowCyt score: | 61.05% |
| Primary researcher: | Sam Kimmey | PI/manager: | Sam Kimmey | Uploaded by: | Sam Kimmey |
| Experiment dates: | 2016-12-15 - 2017-01-20 | Dataset uploaded: | Oct 2018 | Last updated: | May 2019 |
| Keywords: | [mass cytometry] [proliferation] [bone marrow hematopoiesis] [biosynthesis] [translation activity] [transcription activity] | Manuscripts: | [30862852] [PMC6414513] | ||
| Organizations: |
Stanford University, Stanford, CA (USA)
|
||||
| Purpose: | To assess the global activity of DNA, RNA, and protein synthesis in individual bone marrow cells. In parallel, cell type specific markers were measured to characterize these activities in major developmental sub-populations present during hematopoiesis in healthy human bone marrow. | ||||
| Conclusion: | Healthy bone marrow immune cells demonstrate variable activity of DNA, RNA, and protein synthesis, dependent on cell identity, proliferation, and developmental timing. Of note, we observe a the highly proliferative erythroid lineage is among the populations with highest RNA and protein synthesis. Interestingly, we also observe in plasma cells, differentiated B cells tasked with maintaining humoral immunity with antibody production, a high activity of protein synthesis in the absence of de novo RNA synthesis. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Antibodies used in this experiment are validated and titrated using positive and negative control cell lines and/or primary immune samples. Control experiments blocking incorporation of precursor metabolic molecules confirmed specificity of their labeling in human bone marrow cells. | ||||
Experiment variables
| Individuals | |
|---|---|
| · Donor 1 | BM6796_concat_CD45 hi.fcs · BM6796_CD45 hi_B. Erythroblast.fcs · BM6796_CD45 hi_Basophils.fcs · BM6796_CD45 hi_CD14+ Monocyte.fcs · BM6796_CD45 hi_CD4+ Memory.fcs · BM6796_CD45 hi_CD4+ Naive.fcs · BM6796_CD45 hi_CD8a+ Memory.fcs · BM6796_CD45 hi_CD8a+ Naive.fcs · BM6796_CD45 hi_Double Negative T cells.fcs · BM6796_CD45 hi_Early Immature B.fcs · BM6796_CD45 hi_Early Progenitor.fcs · BM6796_CD45 hi_GMP.fcs · BM6796_CD45 hi_HSC.fcs · BM6796_CD45 hi_Immature B.fcs · BM6796_CD45 hi_Intermediate Progenitor.fcs · BM6796_CD45 hi_Late Progenitor.fcs · BM6796_CD45 hi_MEP.fcs · BM6796_CD45 hi_Monoblast.fcs · BM6796_CD45 hi_Myeloid.fcs · BM6796_CD45 hi_NK.fcs · BM6796_CD45 hi_NKT (CD3, CD7, CD4neg, CD8neg).fcs · BM6796_CD45 hi_Neutrophils.fcs · BM6796_CD45 hi_Plasma.fcs · BM6796_CD45 hi_Platelet.fcs · BM6796_CD45 hi_Polychromatic Erythroblast.fcs · BM6796_CD45 hi_Pre B I.fcs · BM6796_CD45 hi_Pre B II.fcs · BM6796_CD45 hi_Pre pDCs.fcs · BM6796_CD45 hi_Pro B.fcs · BM6796_CD45 hi_Pro Erythroblast.fcs · BM6796_CD45 hi_Promonocyte.fcs · BM6796_CD45 hi_Promyelo_myelocyte.fcs · BM6796_CD45 hi_cDC.fcs · BM6796_CD45 hi_pDCs.fcs |
| · Donor 2 | BM6814-concat_CD45 hi.fcs · BM6814_CD45 hi_B. Erythroblast.fcs · BM6814_CD45 hi_Basophils.fcs · BM6814_CD45 hi_CD14+ Monocyte.fcs · BM6814_CD45 hi_CD4+ Memory.fcs · BM6814_CD45 hi_CD4+ Naive.fcs · BM6814_CD45 hi_CD8a+ Memory.fcs · BM6814_CD45 hi_CD8a+ Naive.fcs · BM6814_CD45 hi_Double Negative T cells.fcs · BM6814_CD45 hi_Early Immature B.fcs · BM6814_CD45 hi_Early Progenitor.fcs · BM6814_CD45 hi_GMP.fcs · BM6814_CD45 hi_HSC.fcs · BM6814_CD45 hi_Immature B.fcs · BM6814_CD45 hi_Intermediate Progenitor.fcs · BM6814_CD45 hi_Late Progenitor.fcs · BM6814_CD45 hi_MEP.fcs · BM6814_CD45 hi_Monoblast.fcs · BM6814_CD45 hi_Myeloid.fcs · BM6814_CD45 hi_NK.fcs · BM6814_CD45 hi_NKT (CD3, CD7, CD4neg, CD8neg).fcs · BM6814_CD45 hi_Neutrophils.fcs · BM6814_CD45 hi_Plasma.fcs · BM6814_CD45 hi_Platelet.fcs · BM6814_CD45 hi_Polychromatic Erythroblast.fcs · BM6814_CD45 hi_Pre B I.fcs · BM6814_CD45 hi_Pre B II.fcs · BM6814_CD45 hi_Pre pDCs.fcs · BM6814_CD45 hi_Pro B.fcs · BM6814_CD45 hi_Pro Erythroblast.fcs · BM6814_CD45 hi_Promonocyte.fcs · BM6814_CD45 hi_Promyelo_myelocyte.fcs · BM6814_CD45 hi_cDC.fcs · BM6814_CD45 hi_pDCs.fcs |
