Experiment Overview
| Repository ID: | FR-FCM-ZYS4 | Experiment name: | MMY2002 / SIRIUS Clinical Trial | MIFlowCyt score: | 51.50% |
| Primary researcher: | Yann Abraham | PI/manager: | Greet Vanhoof | Uploaded by: | Yann Abraham |
| Experiment dates: | 2015-04-27 - 2015-06-02 | Dataset uploaded: | Nov 2018 | Last updated: | Mar 2019 |
| Keywords: | [mass cytometry] [CyTOF] [multiple myeloma] [clinical trial] [daratumumab] [darzalex] | Manuscripts: | [30536810] |
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| Organizations: |
Janssen Pharmaceutical Companies of Johnson & Johnson, Janssen Research & Development, Turnhout, (Belgium)
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| Purpose: | The objective of this study was to identify novel changes in immune cell phenotypes that correlate with the efficacy and depth of response observed during treatment with daratumumab in patients with relapsed refractory multiple myeloma. | ||||
| Conclusion: | CyTOF® analysis of patient samples from studies of daratumumab therapy highlight the immune-modulatory mechanism of action of daratumumab. T-cell changes toward a cytolytic, granzyme B+ phenotype observed in WB and BM and increased expression of the activation markers on NK cells, such as CD69, support an adaptive response in patients that may contribute to the depth of response observed in patients treated with daratumumab | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each experiment includes a full panel stained WB healthy volunteer control sample, a panel minus CD38 metal (FMO) stained control WB sample and a full panel stained PBMC sample. Data acquired on CyTOF® were collected in an FCS file format and normalized using CyTOF® Software 6.5.358 for Stand-Alone Processing Workstations (Fluidigm). To ensure that every cell population was represented, the analysis included only samples in which at least 10,000 events in the lymphocyte/monocyte population had been recorded. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Control | 20141104-WB_1_Lympho mono .fcs · 20141104-WB_2_Lympho mono .fcs · 20141104-WB_3_Lympho mono .fcs · 20141104-WB_4_Lympho mono .fcs · 20141104-WB_6_Lympho mono .fcs · 20141104-WB_8_Lympho mono .fcs · 20141104-WB_9_Lympho mono .fcs · 20141104-WB_conc_5_Lympho mono .fcs · 20141104-WB_conc_7_Lympho mono .fcs |
| · Sample | 23255901422_2_Lympho mono .fcs · 2564846033_2_Lympho mono .fcs · 2989177233_1_Lympho mono .fcs · 2989177433_conc_1_Lympho mono .fcs · 2989190433_2_Lympho mono .fcs · 3190133333_1_Lympho mono .fcs · 3205294218_1_Lympho mono .fcs · 3211654233_1_Lympho mono .fcs · 3211654433_1_Lympho mono .fcs · 3218404222_1_Lympho mono .fcs · 3221988333_2_Lympho mono .fcs · 3242909233_3_Lympho mono .fcs · 3265211033_1_Lympho mono .fcs · 3265211233_2_Lympho mono .fcs · 3265571322_2_Lympho mono .fcs · 3280974218_2_Lympho mono .fcs · 3286648033_1_Lympho mono .fcs · 3319210133_1_Lympho mono .fcs · 3320471028_1_Lympho mono .fcs · 3323921122_1_Lympho mono .fcs · 3327808122_2_Lympho mono .fcs · 3328015222_1_Lympho mono .fcs · 3328015322_2_Lympho mono .fcs · 3329245522_2_Lympho mono .fcs · 3330863833_2_Lympho mono .fcs · 3330864733_2_Lympho mono .fcs · 3331191122_3_Lympho mono .fcs · 3331589922_2_Lympho mono .fcs · 3331590122_conc_1_Lympho mono .fcs · 3331747222_1_Lympho mono .fcs · 3334975622_1_Lympho mono .fcs · 3335975318_1_Lympho mono .fcs · 3336045222_2_Lympho mono .fcs · 3337214133_1_Lympho mono .fcs · 3337525022_1_Lympho mono .fcs · 3339167022_1_Lympho mono .fcs · 3339194022_1_Lympho mono .fcs · 3345145633_1_Lympho mono .fcs · 3346408623_1_Lympho mono .fcs · 3347287622_1_Lympho mono .fcs · 3348717922_2_Lympho mono .fcs · 3348839922_2_Lympho mono .fcs · 3350595833_1_Lympho mono .fcs · 3350656122_1_Lympho mono .fcs · 3350656422_2_Lympho mono .fcs · 3352921522_2_Lympho mono .fcs · 3352921622_1_Lympho mono .fcs · 3388492722_1_Lympho mono .fcs · 3407568922_1_Lympho mono .fcs · 3409085422_2_Lympho mono .fcs · 3463789222_1_Lympho mono .fcs · 3475113718_2_Lympho mono .fcs · 3493694518_2_Lympho mono .fcs |
