Experiment Overview
| Repository ID: | FR-FCM-ZYUF | Experiment name: | Broad immune phenotyping of innate and adaptive leukocytes in the brain, spleen, and bone marrow of an orthotopic murine glioblastoma model by mass cytometry | MIFlowCyt score: | 84.56% |
| Primary researcher: | Jan Verhoeff | PI/manager: | Juan J. Garcia Vallejo | Uploaded by: | Jan Verhoeff |
| Experiment dates: | 2018-06-01 - 2018-12-07 | Dataset uploaded: | Jan 2019 | Last updated: | Jan 2019 |
| Keywords: | [spleen] [mass cytometry] [glioblastoma] [mouse] [bone marrow] [brain] | Manuscripts: | |||
| Organizations: |
Amsterdam UMC - VUmc, Molecular Cell Biology & Immunology, Amsterdam, (Nederland)
|
||||
| Purpose: | Optimization of a mass cytometry panel for immunophenotyping of murine glioblastoma infiltrating leukocytes | ||||
| Conclusion: | The panel has been optimized with respect to marker selection, antibody clone usage, antibody-metal pairing, and antibody concentration, and has room for additional markers by filling in a number of free channels. | ||||
| Comments: | None | ||||
| Funding: | Dutch Institute for Chemical Immunology (ICI) and Cancer Center Amsterdam | ||||
| Quality control: | Titration with 8 2-fold dilutions | ||||
Experiment variables
| Doses | |
|---|---|
| · 4x | 20181203_Panel1_T1.fcs · 20181203_Panel2_T1.fcs · 20181203_Panel3_T1.fcs |
| · 2x | 20181203_Panel1_T2.fcs · 20181203_Panel2_T2.fcs · 20181203_Panel3_T2.fcs |
| · 1x | 20181203_Panel1_T3.fcs · 20181203_Panel2_T3.fcs · 20181203_Panel3_T3.fcs |
| · 0.5x | 20181203_Panel1_T4.fcs · 20181203_Panel2_T4.fcs · 20181203_Panel3_T4.fcs |
| · 0.25x | 20181203_Panel1_T5.fcs · 20181203_Panel2_T5.fcs · 20181203_Panel3_T5.fcs |
| · 0.125x | 20181203_Panel1_T6.fcs · 20181203_Panel2_T6.fcs · 20181203_Panel3_T6.fcs |
| · 0.0625x | 20181203_Panel1_T7.fcs · 20181203_Panel2_T7.fcs · 20181203_Panel3_T7.fcs |
| · 0.03125x | 20181203_Panel1_T8.fcs · 20181203_Panel2_T8.fcs · 20181203_Panel3_T8.fcs |
| · Experimental concentrations | c03_20180629_BRAIN.fcs · c09_20180629_BRAIN.fcs · c10_20180628_SPLEEN.fcs · c10_20180629_BONEMARROW.fcs · c10_20180629_BRAIN.fcs |
| Sample Type | |
|---|---|
| · Brain | c03_20180629_BRAIN.fcs · c09_20180629_BRAIN.fcs · c10_20180629_BRAIN.fcs |
| · Spleen | 20181203_Panel1_T1.fcs · 20181203_Panel1_T2.fcs · 20181203_Panel1_T3.fcs · 20181203_Panel1_T4.fcs · 20181203_Panel1_T5.fcs · 20181203_Panel1_T6.fcs · 20181203_Panel1_T7.fcs · 20181203_Panel1_T8.fcs · 20181203_Panel2_T1.fcs · 20181203_Panel2_T2.fcs · 20181203_Panel2_T3.fcs · 20181203_Panel2_T4.fcs · 20181203_Panel2_T5.fcs · 20181203_Panel2_T6.fcs · 20181203_Panel2_T7.fcs · 20181203_Panel2_T8.fcs · 20181203_Panel3_T1.fcs · 20181203_Panel3_T2.fcs · 20181203_Panel3_T3.fcs · 20181203_Panel3_T4.fcs · 20181203_Panel3_T5.fcs · 20181203_Panel3_T6.fcs · 20181203_Panel3_T7.fcs · 20181203_Panel3_T8.fcs · c10_20180628_SPLEEN.fcs |
| · Bone marrow | c10_20180629_BONEMARROW.fcs |
