Experiment Overview
| Repository ID: | FR-FCM-ZYUV | Experiment name: | Immune Cell Differentiation by IdU Pulse Chase | MIFlowCyt score: | 77.44% |
| Primary researcher: | Ratnadeep Mukherjee | PI/manager: | Ratnadeep Mukherjee | Uploaded by: | Ratnadeep Mukherjee |
| Experiment dates: | 2017-06-23 - 2019-01-26 | Dataset uploaded: | Jan 2019 | Last updated: | May 2019 |
| Keywords: | [differentiation] [mass cytometry] [haematopoiesis] [IdU staining] | Manuscripts: | [31150166] |
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| Organizations: |
National Cancer Institute, Cancer & Inflammation Program, Bethesda, MD (20892)
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| Purpose: | Tracking the dynamics of immune cell differentiation in mouse bone marrow by IdU pulse chase and mass cytometry | ||||
| Conclusion: | We were able to track the IdU signal across samples and populations in accordance with our mathematical model. | ||||
| Comments: | None | ||||
| Funding: | This work was supported by Human Frontier Science Program grant LT000123/2014 (Amir Erez), the Gordon Moore foundation and by the Intramural Research Program of the NCI, NIH | ||||
| Quality control: | We made 2 separate QC measures: 1) Using HAL-x (Fig.2 in our manuscript) 2) Checking for phenotypic stability (Fig.3 in our manuscript) | ||||
Experiment variables
| Sample Type | |
|---|---|
| · 0hr | 0hr_r1_m0_170623.FCS · 0hr_r2_m0_20170624.fcs · 0hr_r3_m0_20170625.fcs |
| · 1hr | 1hr_r1_m1_170623.FCS · 1hr_r2_m31_20170625.fcs |
| · 12hr | 12hr_r1_m2_170623.FCS · 12hr_r2_m16_20170624.fcs |
| · 24hr | 24hr_r1_m3_170623.FCS · 24hr_r2_m17_20170624.fcs · 24hr_r3_m32_20170625.fcs |
| · 36hr | 36hr_r1_m18_20170624.fcs · 36hr_r2_m33_20170625.fcs |
| · 48hr | 48hr_r1_m4_170623.FCS · 48hr_r2_m34_20170625.fcs |
| · 60hr | 60hr_r1_m5_170623.FCS · 60hr_r2_m19_20170624.fcs |
| · 72hr | 72hr_r1_m6_170623.FCS · 72hr_r2_m20_20170624.fcs · 72hr_r3_m35_20170625.fcs |
| · 84hr | 84hr_r1_m21_20170624.fcs · 84hr_r2_m36_20170625.fcs |
| · 96hr | 96hr_r1_m7_170623.FCS · 96hr_r2_m37_20170625.fcs |
| · 108hr | 108hr_r1_m8_170623.FCS · 108hr_r2_m22_20170624.fcs |
| · 120hr | 120hr_r1_m9_170623.FCS · 120hr_r2_m23_20170624.fcs · 120hr_r3_m38_20170625.fcs |
| · 132hr | 132hr_r1_m24_20170624.fcs · 132hr_r2_m39_20170625.fcs |
| · 144hr | 144hr_r1_m10_170623.FCS · 144hr_r2_m40_20170625.fcs |
| · 156hr | 156hr_r1_m11_170623.FCS · 156hr_r2_m25_20170624.fcs |
| · 168hr | 168hr_r1_m12_170623.FCS · 168hr_r2_m26_20170624.fcs · 168hr_r3_m41_20170625.fcs |
| · 180hr | 180hr_r1_m27_20170624.fcs · 180hr_r2_m42_20170625.fcs |
| · 192hr | 192hr_r1_m13_170623.FCS · 192hr_r2_m43_20170625.fcs |
| · 204hr | 204hr_r1_m14_170623.FCS · 204hr_r2_m28_20170624.fcs |
| · 216hr | 216hr_r1_m15_170623.FCS · 216hr_r2_m29_20170624.fcs · 216hr_r3_m44_20170625.fcs |
| · 228hr | 228hr_r1_m30_20170624.fcs · 228hr_r2_m45_20170625.fcs |
