Experiment Overview
| Repository ID: | FR-FCM-ZYVH | Experiment name: | Cytometric fingerprints of gut microbiota predict Crohn's disease state | MIFlowCyt score: | 50.75% |
| Primary researcher: | Peter Rubbens | PI/manager: | Nico Boon | Uploaded by: | Peter Rubbens |
| Experiment dates: | 2019-02-18 - 2019-05-24 | Dataset uploaded: | Feb 2019 | Last updated: | May 2019 |
| Keywords: | [flow cytometry] [16S rDNA sequencing] [microbial diversity] [Cytometric fingerprinting] [gut] [Supervised Machine Learning] | Manuscripts: | |||
| Organizations: |
Ghent University, Centre for Microbial Ecology and Technology, Gent, (Belgium)
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| Purpose: | Gut microbiota were analyzed using flow cytometry for patients classified by Crohn's disease (n = 29) versus a healthy control (n = 66) group. Cytometric diversity was evaluated as a biormarker, and compared with the statistical power derived from taxonomic diversity based on 16S rRNA gene amplicon sequencing. | ||||
| Conclusion: | Cytometric diversity of human gut microbiata reveals highly significant differences between the CD and HC group. Consequently, patient status can be predicted. | ||||
| Comments: | None | ||||
| Funding: | Peter Rubbens: BOFSTA2015000501 Ruben Props: BOFDOC2015000601 | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · Healthy control | A01 DC31_HighQ.fcs · A01 DC54_HighQ.fcs · A01 DC56_HighQ.fcs · A01 DC60_HighQ.fcs · A01 DC66_HighQ.fcs · A01 DC88_HighQ.fcs · A02 DC31_HighQ.fcs · A02 DC54_HighQ.fcs · A02 DC56_HighQ.fcs · A02 DC60_HighQ.fcs · A02 DC66_HighQ.fcs · A02 DC88_HighQ.fcs · A03 DC31_HighQ.fcs · A03 DC54_HighQ.fcs · A03 DC56_HighQ.fcs · A03 DC60_HighQ.fcs · A03 DC66_HighQ.fcs · A03 DC88_HighQ.fcs · A04 DC31_HighQ.fcs · A04 DC54_HighQ.fcs · A04 DC56_HighQ.fcs · A04 DC60_HighQ.fcs · A04 DC66_HighQ.fcs · A04 DC88_HighQ.fcs · A05 DC31_HighQ.fcs · A05 DC54_HighQ.fcs · A05 DC56_HighQ.fcs · A05 DC60_HighQ.fcs · A05 DC66_HighQ.fcs · A05 DC88_HighQ.fcs · A06 DC31_HighQ.fcs · A06 DC54_HighQ.fcs · A06 DC56_HighQ.fcs · A06 DC60_HighQ.fcs · A06 DC66_HighQ.fcs · A06 DC88_HighQ.fcs · A07 DC32_HighQ.fcs · A07 DC37_HighQ.fcs · A07 DC57_HighQ.fcs · A07 DC63_HighQ.fcs · A07 DC69_HighQ.fcs · A07 DC89_HighQ.fcs · A08 DC32_HighQ.fcs · A08 DC37_HighQ.fcs · A08 DC57_HighQ.fcs · A08 DC63_HighQ.fcs · A08 DC66_HighQ.fcs · A08 DC89_HighQ.fcs · A09 DC32_HighQ.fcs · A09 DC37_HighQ.fcs · A09 DC57_HighQ.fcs · A09 DC63_HighQ.fcs · A09 DC66_HighQ.fcs · A09 DC89_HighQ.fcs · A10 DC32_HighQ.fcs · A10 DC37_HighQ.fcs · A10 DC57_HighQ.fcs · A10 DC63_HighQ.fcs · A10 DC66_HighQ.fcs · A10 DC89_HighQ.fcs · A11 DC32_HighQ.fcs · A11 DC37_HighQ.fcs · A11 DC57_HighQ.fcs · A11 DC63_HighQ.fcs · A11 DC66_HighQ.fcs · A11 DC89_HighQ.fcs · A12 DC32_HighQ.fcs · A12 DC37_HighQ.fcs · A12 DC57_HighQ.fcs · A12 DC63_HighQ.fcs · A12 DC66_HighQ.fcs · A12 DC89_HighQ.fcs · B01 DC33_HighQ.fcs · B01 DC58_HighQ.fcs · B01 DC61_HighQ.fcs · B01 DC68_HighQ.fcs · B01 DC70_HighQ.fcs · B01 DC90_HighQ.fcs · B02 DC33_HighQ.fcs · B02 DC58_HighQ.fcs · B02 DC61_HighQ.fcs · B02 DC68_HighQ.fcs · B02 DC70_HighQ.fcs · B02 DC90_HighQ.fcs · B03 DC33_HighQ.fcs · B03 DC58_HighQ.fcs · B03 DC61_HighQ.fcs · B03 DC68_HighQ.fcs · B03 DC70_HighQ.fcs · B03 DC90_HighQ.fcs · B04 DC33_HighQ.fcs · B04 DC58_HighQ.fcs · B04 DC61_HighQ.fcs · B04 DC68_HighQ.fcs · B04 DC70_HighQ.fcs · B04 DC90_HighQ.fcs · B05 DC33_HighQ.fcs · B05 DC58_HighQ.fcs · B05 DC61_HighQ.fcs · B05 DC68_HighQ.fcs · B05 DC70_HighQ.fcs · B05 DC90_HighQ.fcs · B06 DC33_HighQ.fcs · B06 DC58_HighQ.fcs · B06 DC61_HighQ.fcs · B06 DC68_HighQ.fcs · B06 DC70_HighQ.fcs · B06 DC90_HighQ.fcs · B07 DC34_HighQ.fcs · B07 DC59_HighQ.fcs · B07 DC73_HighQ.fcs · B07 DC91_HighQ.fcs · B08 DC34_HighQ.fcs · B08 DC59_HighQ.fcs · B08 DC73_HighQ.fcs · B08 DC91_HighQ.fcs · B09 DC34_HighQ.fcs · B09 DC59_HighQ.fcs · B09 DC73_HighQ.fcs · B09 DC91_HighQ.fcs · B10 DC34_HighQ.fcs · B10 DC59_HighQ.fcs · B10 DC73_HighQ.fcs · B10 DC91_HighQ.fcs · B11 DC34_HighQ.fcs · B11 DC59_HighQ.fcs · B11 DC73_HighQ.fcs · B11 DC91_HighQ.fcs · B12 DC34_HighQ.fcs · B12 DC59_HighQ.fcs · B12 DC73_HighQ.fcs · B12 DC91_HighQ.fcs · C01 DC35_HighQ.fcs · C01 DC72_HighQ.fcs · C01 DC76_HighQ.fcs · C01 DC93_HighQ.fcs · C01 DC94_HighQ.fcs · C02 DC35_HighQ.fcs · C02 DC72_HighQ.fcs · C02 DC76_HighQ.fcs · C02 DC93_HighQ.fcs · C02 DC94_HighQ.fcs · C03 DC35_HighQ.fcs · C03 DC72_HighQ.fcs · C03 DC76_HighQ.fcs · C03 DC93_HighQ.fcs · C03 DC94_HighQ.fcs · C04 DC35_HighQ.fcs · C04 DC72_HighQ.fcs · C04 DC76_HighQ.fcs · C04 DC93_HighQ.fcs · C04 DC94_HighQ.fcs · C05 DC35_HighQ.fcs · C05 DC72_HighQ.fcs · C05 DC76_HighQ.fcs · C05 DC93_HighQ.fcs · C05 DC94_HighQ.fcs · C06 DC35_HighQ.fcs · C06 DC72_HighQ.fcs · C06 DC76_HighQ.fcs · C06 DC93_HighQ.fcs · C06 DC94_HighQ.fcs · C07 DC36_HighQ.fcs · C07 DC45_HighQ.fcs · C07 DC74_HighQ.fcs · C07 DC79_HighQ.fcs · C07 DC95_HighQ.fcs · C08 DC36_HighQ.fcs · C08 DC45_HighQ.fcs · C08 DC74_HighQ.fcs · C08 DC79_HighQ.fcs · C08 DC95_HighQ.fcs · C09 DC36_HighQ.fcs · C09 DC45_HighQ.fcs · C09 DC74_HighQ.fcs · C09 DC79_HighQ.fcs · C09 DC95_HighQ.fcs · C10 DC36_HighQ.fcs · C10 DC45_HighQ.fcs · C10 DC74_HighQ.fcs · C10 DC79_HighQ.fcs · C10 DC95_HighQ.fcs · C11 DC36_HighQ.fcs · C11 DC45_HighQ.fcs · C11 DC74_HighQ.fcs · C11 DC79_HighQ.fcs · C11 DC95_HighQ.fcs · C12 DC36_HighQ.fcs · C12 DC45_HighQ.fcs · C12 DC74_HighQ.fcs · C12 DC79_HighQ.fcs · C12 DC95_HighQ.fcs · D01 DC38_HighQ.fcs · D01 DC46_HighQ.fcs · D01 DC75_HighQ.fcs · D01 DC92_HighQ.fcs · D02 DC38_HighQ.fcs · D02 DC46_HighQ.fcs · D02 DC75_HighQ.fcs · D02 DC92_HighQ.fcs · D03 DC38_HighQ.fcs · D03 DC46_HighQ.fcs · D03 DC75_HighQ.fcs · D03 DC92_HighQ.fcs · D04 DC38_HighQ.fcs · D04 DC46_HighQ.fcs · D04 DC75_HighQ.fcs · D04 DC92_HighQ.fcs · D05 DC38_HighQ.fcs · D05 DC46_HighQ.fcs · D05 DC75_HighQ.fcs · D05 DC92_HighQ.fcs · D06 DC38_HighQ.fcs · D06 DC46_HighQ.fcs · D06 DC75_HighQ.fcs · D07 DC39_HighQ.fcs · D07 DC47_HighQ.fcs · D07 DC67_HighQ.fcs · D07 DC77_HighQ.fcs · D08 DC39_HighQ.fcs · D08 DC47_HighQ.fcs · D08 DC67_HighQ.fcs · D08 DC77_HighQ.fcs · D09 DC39_HighQ.fcs · D09 DC47_HighQ.fcs · D09 DC67_HighQ.fcs · D09 DC77_HighQ.fcs · D10 DC39_HighQ.fcs · D10 DC47_HighQ.fcs · D10 DC67_HighQ.fcs · D10 DC77_HighQ.fcs · D11 DC39_HighQ.fcs · D11 DC41_HighQ.fcs · D11 DC47_HighQ.fcs · D11 DC77_HighQ.fcs · D11 DC80_HighQ.fcs · D12 DC39_HighQ.fcs · D12 DC41_HighQ.fcs · D12 DC47_HighQ.fcs · D12 DC77_HighQ.fcs · D12 DC80_HighQ.fcs · E01 DC40_HighQ.fcs · E01 DC41_HighQ.fcs · E01 DC49_HighQ.fcs · E01 DC80_HighQ.fcs · E01 DC81_HighQ.fcs · E02 DC40_HighQ.fcs · E02 DC41_HighQ.fcs · E02 DC49_HighQ.fcs · E02 DC80_HighQ.fcs · E02 DC81_HighQ.fcs · E03 DC40_HighQ.fcs · E03 DC41_HighQ.fcs · E03 DC49_HighQ.fcs · E03 DC80_HighQ.fcs · E03 DC81_HighQ.fcs · E04 DC40_HighQ.fcs · E04 DC41_HighQ.fcs · E04 DC49_HighQ.fcs · E04 DC80_HighQ.fcs · E04 DC81_HighQ.fcs · E05 DC40_HighQ.fcs · E05 DC49_HighQ.fcs · E05 DC55_HighQ.fcs · E05 DC81_HighQ.fcs · E05 DC84_HighQ.fcs · E06 DC40_HighQ.fcs · E06 DC49_HighQ.fcs · E06 DC55_HighQ.fcs · E06 DC81_HighQ.fcs · E06 DC84_HighQ.fcs · E07 DC42_HighQ.fcs · E07 DC50_HighQ.fcs · E07 DC55_HighQ.fcs · E07 DC82_HighQ.fcs · E07 DC84_HighQ.fcs · E08 DC42_HighQ.fcs · E08 DC50_HighQ.fcs · E08 DC55_HighQ.fcs · E08 DC82_HighQ.fcs · E08 DC84_HighQ.fcs · E09 DC42_HighQ.fcs · E09 DC50_HighQ.fcs · E09 DC55_HighQ.fcs · E09 DC82_HighQ.fcs · E09 DC84_HighQ.fcs · E10 DC42_HighQ.fcs · E10 DC50_HighQ.fcs · E10 DC55_HighQ.fcs · E10 DC82_HighQ.fcs · E10 DC84_HighQ.fcs · E11 DC42_HighQ.fcs · E11 DC50_HighQ.fcs · E11 DC78_HighQ.fcs · E11 DC82_HighQ.fcs · E11 DC85_HighQ.fcs · E12 DC42_HighQ.fcs · E12 DC50_HighQ.fcs · E12 DC78_HighQ.fcs · E12 DC82_HighQ.fcs · E12 DC85_HighQ.fcs · F01 DC43_HighQ.fcs · F01 DC44_HighQ.fcs · F01 DC62_HighQ.fcs · F01 DC78_HighQ.fcs · F01 DC85_HighQ.fcs · F02 DC43_HighQ.fcs · F02 DC44_HighQ.fcs · F02 DC62_HighQ.fcs · F02 DC78_HighQ.fcs · F02 DC85_HighQ.fcs · F03 DC43_HighQ.fcs · F03 DC44_HighQ.fcs · F03 DC62_HighQ.fcs · F03 DC78_HighQ.fcs · F03 DC85_HighQ.fcs · F04 DC43_HighQ.fcs · F04 DC44_HighQ.fcs · F04 DC62_HighQ.fcs · F04 DC78_HighQ.fcs · F04 DC85_HighQ.fcs · F05 DC43_HighQ.fcs · F05 DC44_HighQ.fcs · F05 DC62_HighQ.fcs · F05 DC83_HighQ.fcs · F05 DC87_HighQ.fcs · F06 DC43_HighQ.fcs · F06 DC44_HighQ.fcs · F06 DC62_HighQ.fcs · F06 DC83_HighQ.fcs · F06 DC87_HighQ.fcs · F07 DC48_HighQ.fcs · F07 DC51_HighQ.fcs · F07 DC65_HighQ.fcs · F07 DC83_HighQ.fcs · F07 DC87_HighQ.fcs · F08 DC48_HighQ.fcs · F08 DC51_HighQ.fcs · F08 DC65_HighQ.fcs · F08 DC83_HighQ.fcs · F08 DC87_HighQ.fcs · F09 DC48_HighQ.fcs · F09 DC51_HighQ.fcs · F09 DC65_HighQ.fcs · F09 DC83_HighQ.fcs · F09 DC87_HighQ.fcs · F10 DC48_HighQ.fcs · F10 DC51_HighQ.fcs · F10 DC65_HighQ.fcs · F10 DC83_HighQ.fcs · F10 DC87_HighQ.fcs · F11 DC48_HighQ.fcs · F11 DC51_HighQ.fcs · F11 DC65_HighQ.fcs · F11 DC86_HighQ.fcs · F12 DC48_HighQ.fcs · F12 DC51_HighQ.fcs · F12 DC65_HighQ.fcs · F12 DC86_HighQ.fcs · G01 DC30_HighQ.fcs · G01 DC53_HighQ.fcs · G01 DC64_HighQ.fcs · G01 DC71_HighQ.fcs · G01 DC86_HighQ.fcs · G02 DC30_HighQ.fcs · G02 DC53_HighQ.fcs · G02 DC64_HighQ.fcs · G02 DC71_HighQ.fcs · G02 DC86_HighQ.fcs · G03 DC30_HighQ.fcs · G03 DC53_HighQ.fcs · G03 DC64_HighQ.fcs · G03 DC71_HighQ.fcs · G03 DC86_HighQ.fcs · G04 DC30_HighQ.fcs · G04 DC53_HighQ.fcs · G04 DC64_HighQ.fcs · G04 DC71_HighQ.fcs · G04 DC86_HighQ.fcs · G05 DC52_HighQ.fcs · G05 DC53_HighQ.fcs · G05 DC64_HighQ.fcs · G05 DC71_HighQ.fcs · G06 DC52_HighQ.fcs · G06 DC53_HighQ.fcs · G06 DC64_HighQ.fcs · G06 DC71_HighQ.fcs · G07 DC30_HighQ.fcs · G07 DC52_HighQ.fcs · G07 DC67_HighQ.fcs · G08 DC30_HighQ.fcs · G08 DC52_HighQ.fcs · G08 DC67_HighQ.fcs · H01 negative control DC5_HighQ.fcs · H01 negative control DC3_HighQ.fcs · H01 negative control DC4_HighQ.fcs · H01 negative control DC6_HighQ.fcs · H01 negative control DC7_HighQ.fcs · H01 negative control DC8_HighQ.fcs · H01 negative control DC9_HighQ.fcs · H02 negative control DC3_HighQ.fcs · H02 negative control DC4_HighQ.fcs · H02 negative control DC5_HighQ.fcs · H02 negative control DC6_HighQ.fcs · H02 negative control DC7_HighQ.fcs · H02 negative control DC8_HighQ.fcs · H02 negative control DC9_HighQ.fcs · H03 negative control DC3_HighQ.fcs · H03 negative control DC4_HighQ.fcs · H03 negative control DC5_HighQ.fcs · H03 negative control DC6_HighQ.fcs · H03 negative control DC7_HighQ.fcs · H03 negative control DC8_HighQ.fcs · H03 negative control DC9_HighQ.fcs |
| · Crohn's disease | A07 DC01_HighQ.fcs · A07 DC23_HighQ.fcs · A08 DC01_HighQ.fcs · A08 DC23_HighQ.fcs · A09 DC01_HighQ.fcs · A09 DC23_HighQ.fcs · A10 DC01_HighQ.fcs · A10 DC23_HighQ.fcs · A11 DC01_HighQ.fcs · A11 DC23_HighQ.fcs · A12 DC01_HighQ.fcs · A12 DC23_HighQ.fcs · B01 DC04_HighQ.fcs · B02 DC04_HighQ.fcs · B03 DC04_HighQ.fcs · B04 DC04_HighQ.fcs · B05 DC04_HighQ.fcs · B06 DC04_HighQ.fcs · B07 DC06_HighQ.fcs · B07 DC08_HighQ.fcs · B07 DC15_HighQ.fcs · B08 DC06_HighQ.fcs · B08 DC08_HighQ.fcs · B08 DC15_HighQ.fcs · B09 DC06_HighQ.fcs · B09 DC08_HighQ.fcs · B09 DC15_HighQ.fcs · B10 DC06_HighQ.fcs · B10 DC08_HighQ.fcs · B10 DC15_HighQ.fcs · B11 DC06_HighQ.fcs · B11 DC08_HighQ.fcs · B11 DC15_HighQ.fcs · B12 DC06_HighQ.fcs · B12 DC08_HighQ.fcs · B12 DC15_HighQ.fcs · C01 DC10_HighQ.fcs · C01 DC12_HighQ.fcs · C02 DC10_HighQ.fcs · C02 DC12_HighQ.fcs · C03 DC10_HighQ.fcs · C03 DC12_HighQ.fcs · C03 DC16_HighQ.fcs · C04 DC10_HighQ.fcs · C04 DC12_HighQ.fcs · C04 DC16_HighQ.fcs · C05 DC10_HighQ.fcs · C05 DC12_HighQ.fcs · C05 DC16_HighQ.fcs · C06 DC10_HighQ.fcs · C06 DC12_HighQ.fcs · C06 DC16_HighQ.fcs · C07 DC11_HighQ.fcs · C07 DC17_HighQ.fcs · C07 DC22_HighQ.fcs · C08 DC11_HighQ.fcs · C08 DC17_HighQ.fcs · C08 DC22_HighQ.fcs · C09 DC11_HighQ.fcs · C09 DC17_HighQ.fcs · C09 DC22_HighQ.fcs · C10 DC11_HighQ.fcs · C10 DC17_HighQ.fcs · C10 DC22_HighQ.fcs · C11 DC11_HighQ.fcs · C11 DC17_HighQ.fcs · C11 DC22_HighQ.fcs · C12 DC11_HighQ.fcs · C12 DC17_HighQ.fcs · C12 DC22_HighQ.fcs · D01 DC13_HighQ.fcs · D01 DC18_HighQ.fcs · D02 DC13_HighQ.fcs · D02 DC18_HighQ.fcs · D03 DC13_HighQ.fcs · D03 DC18_HighQ.fcs · D04 DC13_HighQ.fcs · D04 DC18_HighQ.fcs · D05 DC13_HighQ.fcs · D05 DC18_HighQ.fcs · D06 DC13_HighQ.fcs · D06 DC18_HighQ.fcs · D07 DC05_HighQ.fcs · D07 DC14_HighQ.fcs · D07 DC19_HighQ.fcs · D08 DC05_HighQ.fcs · D08 DC14_HighQ.fcs · D08 DC19_HighQ.fcs · D09 DC05_HighQ.fcs · D09 DC14_HighQ.fcs · D09 DC19_HighQ.fcs · D10 DC05_HighQ.fcs · D10 DC14_HighQ.fcs · D10 DC19_HighQ.fcs · D11 DC05_HighQ.fcs · D11 DC14_HighQ.fcs · D11 DC19_HighQ.fcs · D12 DC05_HighQ.fcs · D12 DC14_HighQ.fcs · D12 DC19_HighQ.fcs · E01 DC07_HighQ.fcs · E01 DC20_HighQ.fcs · E01 DC24_HighQ.fcs · E02 DC07_HighQ.fcs · E02 DC20_HighQ.fcs · E02 DC24_HighQ.fcs · E03 DC07_HighQ.fcs · E03 DC20_HighQ.fcs · E03 DC24_HighQ.fcs · E04 DC07_HighQ.fcs · E04 DC20_HighQ.fcs · E04 DC24_HighQ.fcs · E05 DC07_HighQ.fcs · E05 DC20_HighQ.fcs · E05 DC24_HighQ.fcs · E06 DC07_HighQ.fcs · E06 DC20_HighQ.fcs · E06 DC24_HighQ.fcs · E07 DC02_HighQ.fcs · E07 DC09_HighQ.fcs · E07 DC25_HighQ.fcs · E07 DC26_HighQ.fcs · E08 DC02_HighQ.fcs · E08 DC09_HighQ.fcs · E08 DC25_HighQ.fcs · E08 DC26_HighQ.fcs · E09 DC02_HighQ.fcs · E09 DC09_HighQ.fcs · E09 DC25_HighQ.fcs · E09 DC26_HighQ.fcs · E10 DC02_HighQ.fcs · E10 DC09_HighQ.fcs · E10 DC25_HighQ.fcs · E10 DC26_HighQ.fcs · E11 DC02_HighQ.fcs · E11 DC09_HighQ.fcs · E11 DC25_HighQ.fcs · E11 DC26_HighQ.fcs · E12 DC02_HighQ.fcs · E12 DC09_HighQ.fcs · E12 DC25_HighQ.fcs · E12 DC26_HighQ.fcs · F01 DC03_HighQ.fcs · F01 DC21_HighQ.fcs · F01 DC27_HighQ.fcs · F01 DC28_HighQ.fcs · F02 DC03_HighQ.fcs · F02 DC21_HighQ.fcs · F02 DC27_HighQ.fcs · F02 DC28_HighQ.fcs · F03 DC03_HighQ.fcs · F03 DC21_HighQ.fcs · F03 DC27_HighQ.fcs · F03 DC28_HighQ.fcs · F04 DC03_HighQ.fcs · F04 DC21_HighQ.fcs · F04 DC27_HighQ.fcs · F04 DC28_HighQ.fcs · F05 DC03_HighQ.fcs · F05 DC21_HighQ.fcs · F05 DC27_HighQ.fcs · F05 DC28_HighQ.fcs · F06 DC03_HighQ.fcs · F06 DC21_HighQ.fcs · F06 DC27_HighQ.fcs · F06 DC28_HighQ.fcs · F07 DC29_HighQ.fcs · F08 DC29_HighQ.fcs · F09 DC29_HighQ.fcs · F10 DC29_HighQ.fcs · F11 DC29_HighQ.fcs · F12 DC29_HighQ.fcs · H01 negative control DC10_HighQ.fcs · H01 negative control DC11_HighQ.fcs · H01 negative control DC1_HighQ.fcs · H01 negative control DC2_HighQ.fcs · H02 negative control DC10_HighQ.fcs · H02 negative control DC11_HighQ.fcs · H02 negative control DC2_HighQ.fcs · H03 negative control DC10_HighQ.fcs · H03 negative control DC11_HighQ.fcs · H03 negative control DC2_HighQ.fcs |
