Experiment Overview
| Repository ID: | FR-FCM-ZZ33 | Experiment name: | Protocol for analyse of expression of IL-1 receptors. | MIFlowCyt score: | 19.50% |
| Primary researcher: | Filipp Vasilyev | PI/manager: | Filipp Vasilyev | Uploaded by: | Filipp Vasilyev |
| Experiment dates: | 2011-02-01 - | Dataset uploaded: | Sep 2012 | Last updated: | Oct 2013 |
| Keywords: | [interleukin-1 receptor; peripheral blood mononuclear cells; quantitative analysis.] | Manuscripts: | [23440375] [PMC3967619] |
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| Organizations: | None | ||||
| Purpose: | Optimize protocol of sample preparation for determining the expression level of IL-1RI and IL-1RII using the QuantiBRITE PE kit, as well as estimating quantitatively the number of membrane-bound receptors on various subpopulations of immunocompetent cells from healthy donors. | ||||
| Conclusion: | We have designed an optimal protocol for estimating the expression level of membrane-bound IL-1Rs, with the aid of QuantiBRITE PE calibration beads. Determination of the mean number of membrane-bound molecules of IL-1Rs using this protocol enables us to obtain precise and reproducible data that are required for the more complete and informative estimation of receptor expression levels. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
