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Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
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Experiment Overview

Repository ID: FR-FCM-ZZ33 Experiment name: Protocol for analyse of expression of IL-1 receptors. MIFlowCyt score: 19.50%
Primary researcher: Filipp Vasilyev PI/manager: Filipp Vasilyev Uploaded by: Filipp Vasilyev
Experiment dates: 2011-02-01 - Dataset uploaded: Sep 2012 Last updated: Oct 2013
Keywords: [interleukin-1 receptor; peripheral blood mononuclear cells; quantitative analysis.] Manuscripts: [23440375] [PMC3967619] Cytotechnologylogo
Organizations: None
Purpose: Optimize protocol of sample preparation for determining the expression level of IL-1RI and IL-1RII using the QuantiBRITE PE kit, as well as estimating quantitatively the number of membrane-bound receptors on various subpopulations of immunocompetent cells from healthy donors.
Conclusion: We have designed an optimal protocol for estimating the expression level of membrane-bound IL-1Rs, with the aid of QuantiBRITE PE calibration beads. Determination of the mean number of membrane-bound molecules of IL-1Rs using this protocol enables us to obtain precise and reproducible data that are required for the more complete and informative estimation of receptor expression levels.
Comments: None
Funding: Not disclosed
Quality control: None
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