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Experiment Overview

Repository ID: FR-FCM-ZZ3N Experiment name: Comparison of aBCR stim reagents in Ramos MIFlowCyt score: 34.00%
Primary researcher: Josef Spidlen PI/manager: Josef Spidlen Uploaded by: Josef Spidlen
Experiment dates: 2011-01-01 - 2011-09-30 Dataset uploaded: Oct 2012 Last updated: Jan 2013
Keywords: [B cells] [trigger] [BCR] [IgM] Manuscripts:
Organizations: BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
Cytobank Inc., Mountain View, California (USA)
Purpose: Measured B cell receptor (BCR) signaling following stimulation by full length polyclonal and monoclonal a-BCR antibodies as well as a-BCR F(ab')2 antibody fragments. The a-BCR reagents were specific for IgM or IgG heavy chain isotype. Ramos cells (IgM isotype) were studied.
Conclusion: None
Comments: Cytobank experiment no. 3937
Funding: Not disclosed
Quality control: None


Experiment variables

Timepoints
· 0' bcellf1.001_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.007_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.013_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.025_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs · bcellf1.103_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.109_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.115_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.127_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs
· 1' bcellf1.002_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.008_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.014_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.026_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs · bcellf1.104_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.110_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.116_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.128_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs
· 2' bcellf1.003_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.009_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.015_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.027_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs · bcellf1.105_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.111_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.117_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.129_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs
· 4' bcellf1.004_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.010_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.016_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.028_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs · bcellf1.106_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.112_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.118_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.130_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs
· 8' bcellf1.005_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.011_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.017_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.029_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs · bcellf1.107_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.113_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.119_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.131_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs
· 16' bcellf1.006_Ramos_B cells_16'_aBCR poly.fcs · bcellf1.012_Ramos_B cells_16'_aBCR F(ab')2.fcs · bcellf1.018_Ramos_B cells_16'_aBCR mono.fcs · bcellf1.030_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs · bcellf1.108_Ramos_B cells_16'_aBCR poly.fcs · bcellf1.114_Ramos_B cells_16'_aBCR F(ab')2.fcs · bcellf1.120_Ramos_B cells_16'_aBCR mono.fcs · bcellf1.132_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs

Sample Type
· Ramos bcellf1.001_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.002_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.003_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.004_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.005_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.006_Ramos_B cells_16'_aBCR poly.fcs · bcellf1.007_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.008_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.009_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.010_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.011_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.012_Ramos_B cells_16'_aBCR F(ab')2.fcs · bcellf1.013_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.014_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.015_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.016_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.017_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.018_Ramos_B cells_16'_aBCR mono.fcs · bcellf1.025_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs · bcellf1.026_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs · bcellf1.027_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs · bcellf1.028_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs · bcellf1.029_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs · bcellf1.030_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs · bcellf1.103_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.104_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.105_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.106_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.107_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.108_Ramos_B cells_16'_aBCR poly.fcs · bcellf1.109_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.110_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.111_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.112_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.113_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.114_Ramos_B cells_16'_aBCR F(ab')2.fcs · bcellf1.115_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.116_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.117_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.118_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.119_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.120_Ramos_B cells_16'_aBCR mono.fcs · bcellf1.127_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs · bcellf1.128_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs · bcellf1.129_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs · bcellf1.130_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs · bcellf1.131_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs · bcellf1.132_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs

Conditions
· aBCR poly bcellf1.001_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.002_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.003_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.004_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.005_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.006_Ramos_B cells_16'_aBCR poly.fcs · bcellf1.103_Ramos_B cells_0'_aBCR poly.fcs · bcellf1.104_Ramos_B cells_1'_aBCR poly.fcs · bcellf1.105_Ramos_B cells_2'_aBCR poly.fcs · bcellf1.106_Ramos_B cells_4'_aBCR poly.fcs · bcellf1.107_Ramos_B cells_8'_aBCR poly.fcs · bcellf1.108_Ramos_B cells_16'_aBCR poly.fcs
· aBCR F(ab')2 bcellf1.007_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.008_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.009_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.010_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.011_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.012_Ramos_B cells_16'_aBCR F(ab')2.fcs · bcellf1.109_Ramos_B cells_0'_aBCR F(ab')2.fcs · bcellf1.110_Ramos_B cells_1'_aBCR F(ab')2.fcs · bcellf1.111_Ramos_B cells_2'_aBCR F(ab')2.fcs · bcellf1.112_Ramos_B cells_4'_aBCR F(ab')2.fcs · bcellf1.113_Ramos_B cells_8'_aBCR F(ab')2.fcs · bcellf1.114_Ramos_B cells_16'_aBCR F(ab')2.fcs
· aBCR mono bcellf1.013_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.014_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.015_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.016_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.017_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.018_Ramos_B cells_16'_aBCR mono.fcs · bcellf1.115_Ramos_B cells_0'_aBCR mono.fcs · bcellf1.116_Ramos_B cells_1'_aBCR mono.fcs · bcellf1.117_Ramos_B cells_2'_aBCR mono.fcs · bcellf1.118_Ramos_B cells_4'_aBCR mono.fcs · bcellf1.119_Ramos_B cells_8'_aBCR mono.fcs · bcellf1.120_Ramos_B cells_16'_aBCR mono.fcs
· aBCR F(ab')2 + H2O2 bcellf1.025_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs · bcellf1.026_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs · bcellf1.027_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs · bcellf1.028_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs · bcellf1.029_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs · bcellf1.030_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs · bcellf1.127_Ramos_B cells_0'_aBCR F(ab')2 + H2O2.fcs · bcellf1.128_Ramos_B cells_1'_aBCR F(ab')2 + H2O2.fcs · bcellf1.129_Ramos_B cells_2'_aBCR F(ab')2 + H2O2.fcs · bcellf1.130_Ramos_B cells_4'_aBCR F(ab')2 + H2O2.fcs · bcellf1.131_Ramos_B cells_8'_aBCR F(ab')2 + H2O2.fcs · bcellf1.132_Ramos_B cells_16'_aBCR F(ab')2 + H2O2.fcs

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