Experiment Overview
| Repository ID: | FR-FCM-ZZ3X | Experiment name: | Human memory T cell purity analysis (pre- and post-FACS sort) | MIFlowCyt score: | 44.75% |
| Primary researcher: | Josef Spidlen | PI/manager: | Josef Spidlen | Uploaded by: | Josef Spidlen |
| Experiment dates: | 2010-04-01 - 2011-11-30 | Dataset uploaded: | Oct 2012 | Last updated: | Jan 2013 |
| Keywords: | [Purity Sort] [T cell] [Ficoll] [CD4] | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
Cytobank Inc., Mountain View, California (USA) |
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| Purpose: | Human blood was subjected to Ficoll while enriching for CD4+ cells using Stem Cell Tech Rosette Sep. Cells were then stained with Fc block, followed by Alexa700-anti-human CD4, PE-anti-human CD45RO, APC-anti-human CD45RA, and a FITC dump panel (CD235a, CD56, CD36, CD19, CD16, CD14). Stained cells were sorted on a FACSAria. | ||||
| Conclusion: | We have found in subsequent experiments that the 0.2-0.5% of cells outside the CD4+ gate are dying cells - purity is increased to greater than 99.9% when a subsequent post-sort stain with live/dead Aqua amine is performed and cells are reanalyzed gating on live/dead aqua amine negative cells. | ||||
| Comments: | Cytobank experiment no. 518. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Pre-Sort | Donor 2 pre-sort.fcs |
| · Post-Sort | Donor 2 mem post-sort.fcs |
