Experiment Overview
| Repository ID: | FR-FCM-ZZ3Y | Experiment name: | OMIP-016: Characterization of Antigen-Responsive macaque and human T-cells (Online Fig 7&8) | MIFlowCyt score: | 84.87% |
| Primary researcher: | Antonio Cosma | PI/manager: | Antonio Cosma | Uploaded by: | Antonio Cosma |
| Experiment dates: | 2012-08-06 - 2012-08-10 | Dataset uploaded: | Sep 2012 | Last updated: | Mar 2013 |
| Keywords: | [Innate Immune Response] [Intracellular cytokines; CD8 T cells] [CD4 T cells; HIV; SIV; vaccines] | Manuscripts: | [23184609] |
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| Organizations: |
CEA, Division of Immuno-Virology, DSV/iMETI, Fontenay-aux-Roses, (France)
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| Purpose: | Evaluate the freeze and thaw procedure | ||||
| Conclusion: | The freeze and thaw procedure can be used as breaking point in the ICS panel described in the OMIP | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The freeze and thaw procedure has been compared to the classical procedure without breaking point. Non stimulated and SEB stimulated samples were used | ||||
Experiment variables
| Conditions | |
|---|---|
| · NS | 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · CTRLS AND BEADS_UNSTAINED_09082012.fcs · DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs |
| · SEB | 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs |
| Individuals | |
|---|---|
| · 20792 | 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs |
| · DONNEUR 2 | DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs |
| Timepoints | |
|---|---|
| · No Break | 20792_20110303_NS_WITHOUT FREEZING ON DAY 2.fcs · 20792_20110303_SEB_WITHOUT FREEZING ON DAY 2.fcs · DONNEUR 2_20091211_NS_WITHOUT FREEZING ON DAY 2.fcs · DONNEUR 2_20091211_SEB_WITHOUT FREEZING ON DAY 2.fcs |
| · Break (30' Thaw) | 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs |
| · Break (40'' Thaw) | 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs · DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs |
