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Experiment Overview

Repository ID: FR-FCM-ZZ3Z Experiment name: OMIP-016: Characterization of Antigen-Responsive macaque and human T-cells (Online Figure 6) MIFlowCyt score: 85.88%
Primary researcher: Antonio Cosma PI/manager: Antonio Cosma Uploaded by: Antonio Cosma
Experiment dates: 2009-09-28 - 2009-10-02 Dataset uploaded: Sep 2012 Last updated: Mar 2013
Keywords: [Innate Immune Response] [CD4 T cells; HIV; SIV; vaccines] [Intracellular cytokines; CD8 T cells; optimization] Manuscripts: [23184609] Cytalogo
Organizations: CEA, Division of Immuno-Virology, DSV/iMETI, Fontenay-aux-Roses, (France)
Purpose: To evaluate the staining intensity of the anti-TNF-a antibody clone MAb11 conjugated to different fluorochromes
Conclusion: The TNF-a Alexafluor 700 resulted to be the best antibody in the context of our panel
Comments: None
Funding: Not disclosed
Quality control: Mock stimulation and SEB stimulation were compared.


Experiment variables

Conditions
· NS CD3A700 5uL TNFa PC7_16322 NS_C01.fcs · CD3A700 5uL TNFaAPC_16322 NS_C02.fcs · CD3PC7 TNFaA700 2uL_16322 NS_C04.fcs
· SEB CD3A700 5uL TNFa PC7_16322 SEB 4ug,2f,mL_D01.fcs · CD3A700 5uL TNFaAPC_16322 SEB 4ug,2f,mL_D02.fcs · CD3PC7 TNFaA700 2uL_16322 SEB 4ug,2f,mL_D04.fcs

Individuals
· 16322 CD3A700 5uL TNFa PC7_16322 NS_C01.fcs · CD3A700 5uL TNFa PC7_16322 SEB 4ug,2f,mL_D01.fcs · CD3A700 5uL TNFaAPC_16322 NS_C02.fcs · CD3A700 5uL TNFaAPC_16322 SEB 4ug,2f,mL_D02.fcs · CD3PC7 TNFaA700 2uL_16322 NS_C04.fcs · CD3PC7 TNFaA700 2uL_16322 SEB 4ug,2f,mL_D04.fcs

Download FCS Files or login and see the dataset in your inbox for further annotation details.