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Experiment Overview

Repository ID: FR-FCM-ZZ45 Experiment name: CD4 Violet dye comparisons MIFlowCyt score: 28.82%
Primary researcher: Barny Abrams PI/manager: Barny Abrams Uploaded by: Barny Abrams
Experiment dates: 2012-02-27 - 2012-11-28 Dataset uploaded: Nov 2012 Last updated: Jun 2013
Keywords: None Manuscripts: [23765858] Cytalogo
Organizations: BD Biosciences, Reagent Applications and Development, San Jose, California (USA)
Purpose: To compare violet-excitable flow reagents of various excitation-emission characteristics from a number of manufacturers including FITC and R-PE for reference
Conclusion: Reagents from BD Biosciences, Life Technologies, and BioLegend showed stain indices (SI) comprising a 3-log range. All violet-excitable reagents exhibited lower SI than R-PE.
Comments: None
Funding: Not disclosed
Quality control: Instrument setup with CS & T beads, and electronic noise-driven PMT settings.
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