Experiment Overview
Repository ID: | FR-FCM-ZZ45 | Experiment name: | CD4 Violet dye comparisons | MIFlowCyt score: | 28.82% |
Primary researcher: | Barny Abrams | PI/manager: | Barny Abrams | Uploaded by: | Barny Abrams |
Experiment dates: | 2012-02-27 - 2012-11-28 | Dataset uploaded: | Nov 2012 | Last updated: | Jun 2013 |
Keywords: | None | Manuscripts: | [23765858] | ||
Organizations: |
BD Biosciences, Reagent Applications and Development, San Jose, California (USA)
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Purpose: | To compare violet-excitable flow reagents of various excitation-emission characteristics from a number of manufacturers including FITC and R-PE for reference | ||||
Conclusion: | Reagents from BD Biosciences, Life Technologies, and BioLegend showed stain indices (SI) comprising a 3-log range. All violet-excitable reagents exhibited lower SI than R-PE. | ||||
Comments: | None | ||||
Funding: | Not disclosed | ||||
Quality control: | Instrument setup with CS & T beads, and electronic noise-driven PMT settings. |