Experiment Overview
| Repository ID: | FR-FCM-ZZ4W | Experiment name: | Analysis of murine NK marker expression before infection (Cohort 1 - Pre-infection) | MIFlowCyt score: | 91.50% |
| Primary researcher: | Jessica Prince | PI/manager: | Michael Brown | Uploaded by: | Jessica Prince |
| Experiment dates: | 2010-06-03 - 2010-06-04 | Dataset uploaded: | Dec 2012 | Last updated: | May 2013 |
| Keywords: | [Ly49G2] [Ly49I/U] [H-2k] [MHC] [NKC] [NKp46] [NCR] [14B11] [4D11] | Manuscripts: | |||
| Organizations: |
University of Virginia, Microbiology, Charlottesville, Virginia (USA)
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| Purpose: | To see what other genetic markers outside of the MHC and NKC regions (in our animals) correlate to viral resistance. | ||||
| Conclusion: | Waiting after all animals (from many more cohorts) are analyzed to make any conclusions. | ||||
| Comments: | A preliminary experiment was performed that showed how NK receptor data analyzed from the blood of a particular mouse (cell frequencies, MFI values) was comparable to the data coming from that same animal's splenocytes. It was after this experiment that we established our system of analyzing NK receptor expression before infection (pre data) via blood, and after infection (post data) via splenocytes, thereby seeing how each animal individually responded to infection. In addition, QTL analysis was done on all of the animals. We already knew the allele of H-2D (and therefore the ability or inability to license Ly49G2 NK cells) would have an effect on viral resistance regarding our animals, but we were interested in what other genetic factors were present. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | All flow data was recollected/re-extracted by a second individual and compared to the data extracted by the primary researcher to ensure accuracy during data compilation. A fraction of the animals had their virus levels re-run to ensure accuracy. In addition, all of the genotypes for our animals were determined twice via High Resolution Melt-PCR (100% match). | ||||
