Experiment Overview
| Repository ID: | FR-FCM-ZZ57 | Experiment name: | Dataset 1-Flow Cytometer Sensitivity: A Quadratic Model | MIFlowCyt score: | 24.00% |
| Primary researcher: | Faysal El Khettabi | PI/manager: | Ryan Brinkman | Uploaded by: | Faysal El Khettabi |
| Experiment dates: | 2011-01-01 - 2011-01-01 | Dataset uploaded: | Jan 2013 | Last updated: | Jan 2013 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
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| Purpose: | Q and B calculation. Dataset 1 was provided by the NIH Vaccine Research Center and involved calibrator beads (Thermo Scientific Duke Standards Corporation) run through an LSRII cytome- ter. MFIs are associated to duke_Beads_xxxV.fcs MESFs are associated to SC-... | ||||
| Conclusion: | None | ||||
| Comments: | Data analysis was conducted using R/BioConductor. We used R’s implementation for K-means clustering to enable the automatic gating and the statistics extraction. The R functions for regression analysis lm was used to fit linear and quadratic regression models. The R function Poly was used to evaluate orthogonal polynomials involved in solving the resulting linear system of equations. We developed a freely available open source R/BioConductor package flowQB to automate the main steps for Q and B calculation, available through CRAN/Bioconductor. It includes a vignette that provides a task-oriented description of its functionality and an executable example that is intended to be used interactively. Thanks to everybody who provided FCS files for this collection! Please email Ryan Brinkman (rbrinkman@bccrc.ca) if you have any questions. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | MESF calibration used p=357217/7102 for voltage 600V, Calibrator Mean =7102 and Calibrator Standard Value =357217. | ||||
