Experiment Overview
| Repository ID: | FR-FCM-ZZ5T | Experiment name: | liposome up taking astrocytes | MIFlowCyt score: | 90.90% |
| Primary researcher: | John Gonzalez | PI/manager: | John Gonzalez | Uploaded by: | John Gonzalez |
| Experiment dates: | 2010-10-29 - 2010-10-29 | Dataset uploaded: | Jan 2013 | Last updated: | Jan 2013 |
| Keywords: | [Astrocytes] [liposomes. myelin-lipids] [CNS inflammation] | Manuscripts: | [23671026] |
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| Organizations: |
Universidad de los Andes, Medicine, Bogotá, DC (Colombia)
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| Purpose: | To determine the ability of a human astrocytoma cell line of internalizing liposomes with myelin-derived lipids: DOPC, POPE, Ceramides (CER), sulfocerebrosides (SCB) and cereborsides (CB) | ||||
| Conclusion: | Up taking of liposomes containing SCB were higher. | ||||
| Comments: | Liposome control DOPC, positive control liposomes POPE | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Settings of each PMT with individual beads and daily CST | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0 | Cell Control_Tiempo 0'.fcs · Cell Control_Tiempo 0.fcs · Vesiculas 30_CB 30'.fcs · Vesiculas 30_CB 30.fcs · Vesiculas 30_CER 30'.fcs · Vesiculas 30_CER 30.fcs · Vesiculas 30_Control '.fcs · Vesiculas 30_Control.fcs · Vesiculas 30_DOPC 30.fcs · Vesiculas 30_DOPC' 30.fcs · Vesiculas 30_POPE 30.fcs · Vesiculas 30_POPE' 30.fcs · Vesiculas 30_SCB 30'.fcs · Vesiculas 30_SCB 30.fcs |
| · 1h | Vesiculas 1h_CB 1h.fcs · Vesiculas 1h_CB' 1h.fcs · Vesiculas 1h_CER 1h.fcs · Vesiculas 1h_CER' 1h.fcs · Vesiculas 1h_Control '.fcs · Vesiculas 1h_Control.fcs · Vesiculas 1h_DOPC 1h.fcs · Vesiculas 1h_DOPC' 1h.fcs · Vesiculas 1h_POPE 1h.fcs · Vesiculas 1h_POPE' 1h.fcs · Vesiculas 1h_SCB 1h.fcs · Vesiculas 1h_SCB' 1h.fcs |
| · 2h | Vesiculas 2h_CB 2h.fcs · Vesiculas 2h_CB' 2h.fcs · Vesiculas 2h_CER 2h.fcs · Vesiculas 2h_CER' 2h.fcs · Vesiculas 2h_Control '.fcs · Vesiculas 2h_Control.fcs · Vesiculas 2h_DOPC 2h.fcs · Vesiculas 2h_DOPC' 2h.fcs · Vesiculas 2h_POPE 2h.fcs · Vesiculas 2h_POPE' 2h.fcs · Vesiculas 2h_SCB 2h.fcs · Vesiculas 2h_SCB' 2h.fcs |
| · 3h | Vesiculas 3h_CB 3h.fcs · Vesiculas 3h_CB' 3h.fcs · Vesiculas 3h_CER 3h.fcs · Vesiculas 3h_CER' 3h.fcs · Vesiculas 3h_Control '.fcs · Vesiculas 3h_Control.fcs · Vesiculas 3h_DOPC 3h.fcs · Vesiculas 3h_DOPC' 3h.fcs · Vesiculas 3h_POPE 3h.fcs · Vesiculas 3h_POPE' 3h.fcs · Vesiculas 3h_SCB 3h.fcs · Vesiculas 3h_SCB' 3h.fcs |
| · 4h | Vesiculas 4h_CB 4h.fcs · Vesiculas 4h_CB' 4h.fcs · Vesiculas 4h_CER 4h.fcs · Vesiculas 4h_CER' 4h.fcs · Vesiculas 4h_Control '.fcs · Vesiculas 4h_Control.fcs · Vesiculas 4h_DOPC 4h.fcs · Vesiculas 4h_DOPC' 4h.fcs · Vesiculas 4h_POPE 4h.fcs · Vesiculas 4h_POPE' 4h.fcs · Vesiculas 4h_SCB 4h.fcs · Vesiculas 4h_SCB' 4h.fcs |
| Conditions | |
|---|---|
| · Tiempo | Cell Control_Tiempo 0'.fcs · Cell Control_Tiempo 0.fcs |
| · CB | Vesiculas 1h_CB 1h.fcs · Vesiculas 1h_CB' 1h.fcs · Vesiculas 1h_SCB 1h.fcs · Vesiculas 1h_SCB' 1h.fcs · Vesiculas 2h_CB 2h.fcs · Vesiculas 2h_CB' 2h.fcs · Vesiculas 2h_SCB 2h.fcs · Vesiculas 2h_SCB' 2h.fcs · Vesiculas 30_CB 30'.fcs · Vesiculas 30_CB 30.fcs · Vesiculas 30_SCB 30'.fcs · Vesiculas 30_SCB 30.fcs · Vesiculas 3h_CB 3h.fcs · Vesiculas 3h_CB' 3h.fcs · Vesiculas 3h_SCB 3h.fcs · Vesiculas 3h_SCB' 3h.fcs · Vesiculas 4h_CB 4h.fcs · Vesiculas 4h_CB' 4h.fcs · Vesiculas 4h_SCB 4h.fcs · Vesiculas 4h_SCB' 4h.fcs |
| · CER | Vesiculas 1h_CER 1h.fcs · Vesiculas 1h_CER' 1h.fcs · Vesiculas 2h_CER 2h.fcs · Vesiculas 2h_CER' 2h.fcs · Vesiculas 30_CER 30'.fcs · Vesiculas 30_CER 30.fcs · Vesiculas 3h_CER 3h.fcs · Vesiculas 3h_CER' 3h.fcs · Vesiculas 4h_CER 4h.fcs · Vesiculas 4h_CER' 4h.fcs |
| · Control | Vesiculas 1h_Control '.fcs · Vesiculas 1h_Control.fcs · Vesiculas 2h_Control '.fcs · Vesiculas 2h_Control.fcs · Vesiculas 30_Control '.fcs · Vesiculas 30_Control.fcs · Vesiculas 3h_Control '.fcs · Vesiculas 3h_Control.fcs · Vesiculas 4h_Control '.fcs · Vesiculas 4h_Control.fcs |
| · DOPC | Vesiculas 1h_DOPC 1h.fcs · Vesiculas 1h_DOPC' 1h.fcs · Vesiculas 2h_DOPC 2h.fcs · Vesiculas 2h_DOPC' 2h.fcs · Vesiculas 30_DOPC 30.fcs · Vesiculas 30_DOPC' 30.fcs · Vesiculas 3h_DOPC 3h.fcs · Vesiculas 3h_DOPC' 3h.fcs · Vesiculas 4h_DOPC 4h.fcs · Vesiculas 4h_DOPC' 4h.fcs |
| · POPE | Vesiculas 1h_POPE 1h.fcs · Vesiculas 1h_POPE' 1h.fcs · Vesiculas 2h_POPE 2h.fcs · Vesiculas 2h_POPE' 2h.fcs · Vesiculas 30_POPE 30.fcs · Vesiculas 30_POPE' 30.fcs · Vesiculas 3h_POPE 3h.fcs · Vesiculas 3h_POPE' 3h.fcs · Vesiculas 4h_POPE 4h.fcs · Vesiculas 4h_POPE' 4h.fcs |
