Experiment Overview
| Repository ID: | FR-FCM-ZZ5V | Experiment name: | Astrocytes response to T cruzi infection | MIFlowCyt score: | 50.00% |
| Primary researcher: | Juan Camilo Vargas-Zambrano | PI/manager: | John Gonzalez | Uploaded by: | Juan Camilo Vargas-Zambrano |
| Experiment dates: | 2011-02-01 - 2012-01-11 | Dataset uploaded: | Jan 2013 | Last updated: | Oct 2013 |
| Keywords: | [Astrocytes] [trypanosoma cruzi] [HLA I] [HLA II] | Manuscripts: | [23579802] |
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| Organizations: |
Universidad de los Andes, Medicine, Bogotá, DC (Colombia)
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| Purpose: | The purpose of this experiment was to determine how human astrocytes response to Trypanosoma cruzi infection. We analyzed the mortality, HLA I and II expression due to the parasite infection. | ||||
| Conclusion: | T cruzi infection caused 3 major effects in cells: 1. Increased HLA I MFI 2. Increased percentage of cells expressing HLA II 3. Increased mortality | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | No labelled control cells, PMT settings with beads and CST | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 2 | 2 dias_Infectado 1.fcs · 2 dias_Infectado 2.fcs · 2 dias_Infectado 3.fcs · 2 dias_Infectado 4.fcs · 2 dias_Infectado 5.fcs · 2 dias_control marcado.fcs · 2 dias_control sin marcar.fcs |
| · 4 | 4 dias_Infectado 1.fcs · 4 dias_Infectado 2.fcs · 4 dias_Infectado 3.fcs · 4 dias_Infectado 4.fcs · 4 dias_Infectado 5.fcs · 4 dias_control marcado.fcs |
| · 6 | 6 dias_Control 1.fcs · 6 dias_Control 2.fcs · 6 dias_Infectado 1.fcs · 6 dias_Infectado 2.fcs · 6 dias_Infectado 3.fcs · 6 dias_Infectado 4.fcs · 6 dias_Infectado 5.fcs |
| Individuals | |
|---|---|
| · Infectado | 2 dias_Infectado 1.fcs · 2 dias_Infectado 2.fcs · 2 dias_Infectado 3.fcs · 2 dias_Infectado 4.fcs · 2 dias_Infectado 5.fcs · 4 dias_Infectado 1.fcs · 4 dias_Infectado 2.fcs · 4 dias_Infectado 3.fcs · 4 dias_Infectado 4.fcs · 4 dias_Infectado 5.fcs · 6 dias_Infectado 1.fcs · 6 dias_Infectado 2.fcs · 6 dias_Infectado 3.fcs · 6 dias_Infectado 4.fcs · 6 dias_Infectado 5.fcs |
| · Control | 2 dias_control marcado.fcs · 2 dias_control sin marcar.fcs · 4 dias_control marcado.fcs · 6 dias_Control 1.fcs · 6 dias_Control 2.fcs |
