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Experiment Overview

Repository ID: FR-FCM-ZZ6B Experiment name: Germinal Center Lymphoma versus Hyperplasia MIFlowCyt score: 61.25%
Primary researcher: Fiona Craig PI/manager: Fiona Craig Uploaded by: Fiona Craig
Experiment dates: 2012-05-01 - 2013-02-22 Dataset uploaded: Feb 2013 Last updated: May 2013
Keywords: [diffuse large B-cell lymphoma] [hyperplasia] [follicular lymphoma] Manuscripts: [23873623] Cytblogo
Organizations: None
Purpose: Data sets to address the question ?What is the best flow cytometric analysis strategy to distinguish germinal center B-cell lymphoma from reactive lymphoid tissue using the 8-color antibody combination: anti-kappa, anti-lambda, CD19, CD20, CD10, CD5, CD38 and CD45 antibodies?? Germinal center lymphoma, n=48. Lymphoid hyperplasia, including germinal center cells, n=52.
Conclusion: Application of the computational tools flowType and RchyOptimyx (cellular hieraRCHY OPYIMization) highlighted the diagnostic utility of identifying CD10 positive, CD38 negative B-cells in the distinction between GC-L and GC-H.
Comments: None
Funding: Not disclosed
Quality control: Clinical laboratory procedures employed to reduce sources of variability including standardized sample preparation and staining, lot-to-lot comparison of reagents, and cloning of instrument settings.


Experiment variables

Individuals
· Lymphoma LNA10_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA11_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA15_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA16_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA17_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA18_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA20_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA21_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA22_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA23_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA24_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA3_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA8_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA100_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA27_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA28_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA29_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA32_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA33_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA37_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA38_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA40_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA42_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA43_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA44_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA45_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA50_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA51_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA52_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA55_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA57_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA59_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA63_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA66_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA72_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA73_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA76_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA77_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA79_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA80_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA82_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA83_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA84_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA85_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA91_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA92_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA93_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA94_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA96_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA97_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA98_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA99_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs
· Hyperplasia LNA12_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA13_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA14_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA19_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA1_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA25_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA2_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA4_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA5_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA6_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA7_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA9_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA26_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA30_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA31_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA34_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA35_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA36_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA39_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA41_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA46_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA47_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA48_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA49_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA53_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA54_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA56_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA58_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA60_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA61_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA62_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA64_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA65_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA67_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA68_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA69_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA70_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA71_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA74_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA75_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA78_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA81_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA86_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA87_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA88_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA89_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA90_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs · LNA95_K,2f,L,2f,CD5,2f,CD19,2f,CD10,2f,CD38,2f,CD20,2f,CD45.fcs

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