Experiment Overview
Repository ID: | FR-FCM-ZZ6K | Experiment name: | Lymphocyte Subsets Reference Ranges in Omanis | MIFlowCyt score: | 48.50% |
Primary researcher: | Adhra Al Mawali | PI/manager: | Adhra Al Mawali | Uploaded by: | Avinash Daniel Pinto |
Experiment dates: | 2010-04-01 - 2010-10-31 | Dataset uploaded: | May 2013 | Last updated: | Aug 2014 |
Keywords: | [flow cytometry] [lymphocyte subsets] [reference ranges] [Oman] [CD3+CD4+] [CD3+CD8+] | Manuscripts: | [23839863] | ||
Organizations: |
The Royal Hospital, Haematology and Blood Transfusion, Muscat, (Sultanate of Oman)
|
||||
Purpose: | The purpose of our study is to identify the reference ranges for healthy Omani donors for T-cell, B-cell and NK-cell lymphocyte subsets with a comprehensive and informative panel of MoAbs with the CD45/SSC log gating strategy, in both males and females in a broad age spectrum. | ||||
Conclusion: | None | ||||
Comments: | None | ||||
Funding: | Not disclosed | ||||
Quality control: | Internal quality control procedures were used to assess instrument parameters and assure accurate results. Daily calibration of the flow cytometer was performed using Flow-check and Flow-set fluorospheres (Beckman Coulter) for optical laser alignment and optimal hydrodynamic focussing settings respectively. An external quality assurance procedure was also implemented through participation in a performance-monitoring network operated by The Royal College of Pathologists of Australasia Quality Assurance (RCPA) programme. |