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Experiment Overview

Repository ID: FR-FCM-ZZ6K Experiment name: Lymphocyte Subsets Reference Ranges in Omanis MIFlowCyt score: 48.50%
Primary researcher: Adhra Al Mawali PI/manager: Adhra Al Mawali Uploaded by: Avinash Daniel Pinto
Experiment dates: 2010-04-01 - 2010-10-31 Dataset uploaded: May 2013 Last updated: Aug 2014
Keywords: [flow cytometry] [lymphocyte subsets] [reference ranges] [Oman] [CD3+CD4+] [CD3+CD8+] Manuscripts: [23839863] Cytalogo
Organizations: The Royal Hospital, Haematology and Blood Transfusion, Muscat, (Sultanate of Oman)
Purpose: The purpose of our study is to identify the reference ranges for healthy Omani donors for T-cell, B-cell and NK-cell lymphocyte subsets with a comprehensive and informative panel of MoAbs with the CD45/SSC log gating strategy, in both males and females in a broad age spectrum.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: Internal quality control procedures were used to assess instrument parameters and assure accurate results. Daily calibration of the flow cytometer was performed using Flow-check and Flow-set fluorospheres (Beckman Coulter) for optical laser alignment and optimal hydrodynamic focussing settings respectively. An external quality assurance procedure was also implemented through participation in a performance-monitoring network operated by The Royal College of Pathologists of Australasia Quality Assurance (RCPA) programme.
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