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Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
We apologize for the inconvenience and appreciate your understanding as we work on upgrading our hardware and improving the overall solution.
Thank you for your patience.

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Experiment Overview

Repository ID: FR-FCM-ZZ6W Experiment name: Fluorescence as an alternative to light-scatter gating strategies to identify frozen-thawed cells with flow cytometry MIFlowCyt score: 19.50%
Primary researcher: Anthony Reardon PI/manager: Janet Elliott Uploaded by: Janet Elliott
Experiment dates: 2012-02-01 - Dataset uploaded: May 2013 Last updated: Aug 2014
Keywords: [flow cytometry] [fluorescence] [membrane integrity] [light scattering] [cryopreservation] [cryobiology] [cell gating] [mitochondria] [mitochondrial membrane potential] [human umbilical vein endothelial cells (HUVEC)] Manuscripts: [24880088] Cryobiologylogo
Organizations: None
Purpose: The objective of this study was to demonstrate that gating strategies based on forward light scattering may introduce inaccuracies in experiments that require the identification of total cell populations. This study also investigates the use of fluorescence-based gating as an alternative strategy to identify all cells in a sample population for cryobiology applications.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: None
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