Experiment Overview
| Repository ID: | FR-FCM-ZZ7S | Experiment name: | Genetic Barcoding Adherent Cell Types | MIFlowCyt score: | 82.00% |
| Primary researcher: | Roland Wolkowicz | PI/manager: | Roland Wolkowicz | Uploaded by: | Roland Wolkowicz |
| Experiment dates: | 2012-09-01 - 2013-07-01 | Dataset uploaded: | Sep 2013 | Last updated: | Oct 2013 |
| Keywords: | [flow cytometry] [Fluorescent proteins] [Genetic barcoding] [Retroviral technology] [Multiplexing] [high throughput capabilities] | Manuscripts: | [24700576] |
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| Organizations: |
San Diego State University, Biology, San Diego, California (USA)
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| Purpose: | Genetic barcoding of adherent mammalian cells utilizing retroviral technology for enhancing the power of multiplexed application, and versatility of different cell types. | ||||
| Conclusion: | The growing number of existing fluorescent proteins and derivates with distinct absorbance/emission spectra, combined wih the growing number of affordable detection devices and lasers, increases the versatility of multiplexing, making fluorescent genetic barcoding a powerful tool for flow cytometry-based analysis. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each figure includes cells with no fluorescent protein as a negative control, allowing for gating. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Normal Conditions | 293s final_Crimson.fcs · 293s final_Tomato.fcs · 293s final_negative.fcs · Huh thawed_E2 Crimson.fcs · Huh thawed_negative.fcs · Huh thawed_tomato.fcs |
| Sample Type | |
|---|---|
| · 293 cells | 293s final_Crimson.fcs · 293s final_Tomato.fcs · 293s final_negative.fcs |
| · Huh cells | Huh thawed_E2 Crimson.fcs · Huh thawed_negative.fcs · Huh thawed_tomato.fcs |
