Experiment Overview
| Repository ID: | FR-FCM-ZZ7T | Experiment name: | Genetically Barcoded SupT1 Cells | MIFlowCyt score: | 79.75% |
| Primary researcher: | Roland Wolkowicz | PI/manager: | Roland Wolkowicz | Uploaded by: | Roland Wolkowicz |
| Experiment dates: | 2012-09-01 - 2013-07-01 | Dataset uploaded: | Sep 2013 | Last updated: | Oct 2013 |
| Keywords: | [flow cytometry] [Fluorescent proteins] [Genetic barcoding] [Retroviral technology] [Multiplexing] [high throughput capabilities] | Manuscripts: | [24700576] |
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| Organizations: |
San Diego State University, Biology, San Diego, California (USA)
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| Purpose: | Genetic barcoding of non-adherent mammalian cells utilizing retroviral technology enahnces the power of mulitplexed applications. SupT1 cells can be separated based on fluorescence intensity, and are stable for long periods of time. | ||||
| Conclusion: | The growing number of existing fluorescent proteins and derivates with distinct absorbance/emission spectra, combined with the growing number of affordable detection devices and lasers, increases the versatility of multiplexing, making fluorescent genetic barcoding a powerful tool for flow cytometry-based analysis. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Each figure includes cells with no fluorescent protein as a negative control allowing for gating. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · SupT1 cells | specimen 1_bright tomato e2 crimson pass.fcs · specimen 1_dim tomato e2 crimson pass.fcs · specimen 1_dim tomato pass.fcs · specimen 1_e2 crimson thawed.fcs · specimen 1_negative pass.fcs · specimen 2_bright tomato thaw orig.fcs · specimen 2_dim tomato e2 crimson thawed.fcs · specimen 2_dim tomato thawed.fcs · specimen 2_e2 crimson pass.fcs · specimen 2_negative thawed.fcs · specimen 3_bright tomato crimson thaw orig.fcs · specimen 3_bright tomato e2 crimson orig.fcs · specimen 3_bright tomato pass.fcs · specimen 3_dim td tomato orig.fcs · specimen 3_negative orig.fcs · specimen 4_bright tomato orig.fcs · specimen 4_dim tomato e2 crimson orig.fcs · specimen 4_e2 crimson orig.fcs · gfp sorts_bright tomato e2 crimson.fcs · gfp sorts_bright tomato.fcs · gfp sorts_dim tomato e2 crimson.fcs · gfp sorts_dim tomato.fcs · gfp sorts_e2 crimson.fcs · gfp sorts_negative.fcs · sort analysis_BC20 ++.fcs · stability of 12 pops and mixing_MIX of 9_001.fcs |
