Experiment Overview
| Repository ID: | FR-FCM-ZZ7V | Experiment name: | Adaptation of Genetically Barcoded Cells to a Cell Based Assay | MIFlowCyt score: | 77.50% |
| Primary researcher: | Roland Wolkowicz | PI/manager: | Roland Wolkowicz | Uploaded by: | Roland Wolkowicz |
| Experiment dates: | 2012-09-01 - 2013-07-01 | Dataset uploaded: | Sep 2013 | Last updated: | Oct 2013 |
| Keywords: | [flow cytometry] [Fluorescent proteins] [Genetic barcoding] [Retroviral technology] [Multiplexing] [high throughput capabilities] [cell based assay] | Manuscripts: | [24700576] |
|
|
| Organizations: |
San Diego State University, Biology, San Diego, California (USA)
|
||||
| Purpose: | To utilize established genetically barcoded mammalian cells for a cell-based assay. Specifically this assay monitors cleavage in the classical secretory pathway and relies on the expression of one or two tags on the cell surface (HA only, or HA and FLAG). | ||||
| Conclusion: | Naive and td Tomato barcoded SupT1 cells at mid and high intensities were used independently express viral proteins known to be cleaved by host enzymes. When mixed samples are analyzed in the APC and FITC channels (for HA and FLAG staining) a combination of positive populations can be observed, distinguishing the cleaved versus the un-cleaved products. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Figure 4 in the manuscript includes and un-cleaved product as a control for staining and cleavage of the cleaved products. | ||||
