Experiment Overview
| Repository ID: | FR-FCM-ZZ8Z | Experiment name: | Detection of IgG, IgM, IgA and C3d attached to erythrocytes by flow cytometry | MIFlowCyt score: | 89.50% |
| Primary researcher: | John Gonzalez | PI/manager: | John Gonzalez | Uploaded by: | John Gonzalez |
| Experiment dates: | 2011-06-01 - 2013-12-01 | Dataset uploaded: | Sep 2013 | Last updated: | Dec 2013 |
| Keywords: | [flow cytometry] [autoimmune hemolytic anemia] [erythrocytes] [immune complexes ] | Manuscripts: | |||
| Organizations: |
Universidad de los Andes, Medicine, Bogotá, DC (Colombia)
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| Purpose: | Design a flow cytometry protocol for simultaneous detection of IgG, IgM, IgA and C3d immune complexes attached to the erythrocytes? membrane in patients with autoinmune hemolytic anemia. | ||||
| Conclusion: | Flow cytometry can be used to identify the presence of IgM, IgG, C3d and IgA immune complexes attached to the erythrocytes’ membrane and allow an approach to physiopathology mechanisms that interact in the development of cellular lysis in AHAI patients. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | 1. Use of Isotype controls for every experiment 2. Performing CST every day | ||||
