Experiment Overview
| Repository ID: | FR-FCM-ZZA4 | Experiment name: | Basophils in human allergic asthma | MIFlowCyt score: | 63.25% |
| Primary researcher: | Dorothea Dijkstra | PI/manager: | Dorothea Dijkstra | Uploaded by: | Dorothea Dijkstra |
| Experiment dates: | 2008-08-01 - 2012-05-01 | Dataset uploaded: | Feb 2014 | Last updated: | Apr 2014 |
| Keywords: | [flow cytometry] [basophils] [Blood] [asthma] [bronchoalveolar lavage] [chipcytometry] | Manuscripts: | [24733663] |
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| Organizations: |
Fraunhofer Institute for Toxicology and Experimental Medicine, Department of Clinical Airway Research, Hannover, (Germany)
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| Purpose: | During asthma attacks, allergens activate sensitized basophils in the lung, thereby aggravating symptoms. Due to the paucity of basophils in bronchial lavage fluid and the lack of specific basophil detection and quantification methods, basophil-directed research in these samples was hampered in the past. This study aimed to establish and validate a flow cytometry-based basophil detection and quantification method for human basophils from bronchoalveolar lavage (BAL) and blood as a prerequisite for a better understanding of their pathogenic contribution and subtyping of asthma phenotypes. | ||||
| Conclusion: | BAL basophils are identifiable as CD123+HLA-DR-CD3-CD14-CD19-CD20-CD56- cells in flow cytometrical analysis. Their identity was validated by Chipcytometry. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The constancy of the flow cytometer's lasers was checked by measureing rainbow beads and calibration beads. To quality control (below, the option is missing): we used chipcytometry to validate our results from the flow cytometry experiments | ||||
