Experiment Overview
| Repository ID: | FR-FCM-ZZA8 | Experiment name: | Mouse Naive Cell Differentiation | MIFlowCyt score: | 89.95% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2011-02-17 - 2011-04-26 | Dataset uploaded: | Mar 2014 | Last updated: | Aug 2014 |
| Keywords: | [IL17; IL22; mouse] | Manuscripts: | |||
| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
|
||||
| Purpose: | To test the effects of cinnabarinic acid on mouse naive CD4 T cell cytokine production under Th17 driving conditions. | ||||
| Conclusion: | Cinnabarinic acid has a significant effect on IL-22 production in vitro in mouse CD4+ T cells. | ||||
| Comments: | See Figure 6A and 6B of Lowe et al. PLoS One 2014. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Individuals | |
|---|---|
| · wild-type | WT_CA 25 B10.fcs · WT_CA 35 B10.fcs · WT_DMSO 25 B10.fcs · WT_DMSO 35 B 10.fcs · day 5 minus gamma_CA 25.fcs · day 5 minus gamma_CA 35.fcs · day 5 minus gamma_DMSO 25.fcs · day 5 minus gamma_DMSO 35.fcs · WT_CA 25 no1.fcs · WT_CA 25 no2.fcs · WT_CA 35 no1.fcs · WT_CA 35 no2.fcs · WT_DMSO 25 no 1.fcs · WT_DMSO 25 no 2.fcs · WT_DMSO 35 no 2.fcs · WT_DMSO 35 no1.fcs · WT_CA 25 2.fcs · WT_CA 25.fcs · WT_CA 35 2.fcs · WT_CA 35.fcs · WT_DMSO 25 2.fcs · WT_DMSO 25.fcs · WT_DMSO 35 2.fcs · WT_DMSO 35.fcs |
| · knockout | day 5 minus gamma_CA 25 KO.fcs · day 5 minus gamma_CA 35 KO.fcs · day 5 minus gamma_DMSO 25 KO.fcs · day 5 minus gamma_DMSO 35 KO.fcs · AHR knockout_CA 25 no1.fcs · AHR knockout_CA 25 no2.fcs · AHR knockout_CA 35 no1.fcs · AHR knockout_CA 35 no2.fcs · AHR knockout_DMSO 25 no 1.fcs · AHR knockout_DMSO 25 no 2.fcs · AHR knockout_DMSO 35 no 2.fcs · AHR knockout_DMSO 35 no1.fcs · AHR Knockout_CA 25.fcs · AHR Knockout_CA 35.fcs · AHR Knockout_DMSO 25.fcs · AHR Knockout_DMSO 35.fcs |
| Conditions | |
|---|---|
| · DMSO 25 µM control | WT_DMSO 25 B10.fcs · day 5 minus gamma_DMSO 25 KO.fcs · day 5 minus gamma_DMSO 25.fcs · AHR knockout_DMSO 25 no 1.fcs · AHR knockout_DMSO 25 no 2.fcs · WT_DMSO 25 no 1.fcs · WT_DMSO 25 no 2.fcs · AHR Knockout_DMSO 25.fcs · WT_DMSO 25 2.fcs · WT_DMSO 25.fcs |
| · DMSO 35 µM control | WT_DMSO 35 B 10.fcs · day 5 minus gamma_DMSO 35 KO.fcs · day 5 minus gamma_DMSO 35.fcs · AHR knockout_DMSO 35 no 2.fcs · AHR knockout_DMSO 35 no1.fcs · WT_DMSO 35 no 2.fcs · WT_DMSO 35 no1.fcs · AHR Knockout_DMSO 35.fcs · WT_DMSO 35 2.fcs · WT_DMSO 35.fcs |
| · CA 25 µM | WT_CA 25 B10.fcs · day 5 minus gamma_CA 25 KO.fcs · day 5 minus gamma_CA 25.fcs · AHR knockout_CA 25 no1.fcs · AHR knockout_CA 25 no2.fcs · WT_CA 25 no1.fcs · WT_CA 25 no2.fcs · AHR Knockout_CA 25.fcs · WT_CA 25 2.fcs · WT_CA 25.fcs |
| · CA 35 µM | WT_CA 35 B10.fcs · day 5 minus gamma_CA 35 KO.fcs · day 5 minus gamma_CA 35.fcs · AHR knockout_CA 35 no1.fcs · AHR knockout_CA 35 no2.fcs · WT_CA 35 no1.fcs · WT_CA 35 no2.fcs · AHR Knockout_CA 35.fcs · WT_CA 35 2.fcs · WT_CA 35.fcs |
