Experiment Overview
| Repository ID: | FR-FCM-ZZAD | Experiment name: | Laccase, CA, KYA, and L-KYN on Mouse cells | MIFlowCyt score: | 82.71% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2011-12-09 - 2012-10-11 | Dataset uploaded: | Mar 2014 | Last updated: | Aug 2014 |
| Keywords: | [mouse T cells] [IL17] [IL22] [KYA] [L-KYN] [laccase] | Manuscripts: | [24498387] [PMC3912126] |
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| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
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| Purpose: | To determine the effect of KYA and L-KYN on IL-22 production in mouse CD4+ T cells versus CA. To determine the effect of laccase activity on IL-22 production in mouse CD4+ T cells. | ||||
| Conclusion: | CA has a greater effect on IL-22 production than KYA and L-KYN. Laccase activity is able to cause IL-22 production. | ||||
| Comments: | See figures 8A and 9E of Lowe et al PLOS One 2014. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · Vehicle | 6 hour_DMSO 10.fcs · 6 hour_DMSO 25.fcs · 6 hour_DMSO 50.fcs · 6h_DMSO 10.fcs · 6h_DMSO 25.fcs · DMSO_10 microM.fcs · DMSO_10.fcs · DMSO_10.fcs-(1) · DMSO_10.fcs-(2) · DMSO_25 microM.fcs · DMSO_25.fcs · DMSO_25.fcs-(1) · DMSO_25.fcs-(2) · DMSO_5.fcs · DMSO_5.fcs-(1) · DMSO_none.fcs · Exp 1_D10.fcs · Exp 1_D25.fcs · Exp 2_D10.fcs · Exp 2_D25.fcs · Exp 2_D5.fcs · beta_DMSO beta 10.fcs · beta_DMSO no PMA.fcs |
| · CA | 6 hour_CA 10.fcs · 6 hour_CA 25.fcs · 6 hour_CA 50.fcs · 6h_CA new 10.fcs · 6h_CA new 25.fcs · CA_10 microM.fcs · CA_10.fcs · CA_25 microM.fcs · CA_25.fcs · CA_25.fcs-(1) · beta_CA 10 beta 10 no PMA.fcs · beta_CA 10 beta 10.fcs · beta_CA 25 beta 10 no PMA.fcs · beta_CA 25 beta 10.fcs |
| · Laccase | 6 hour_laccase 0-1 UperML.fcs · Exp 1_Laccase 0-05.fcs · Exp 1_laccase HK 0-05.fcs · Exp 1_laccase HK 0-1.fcs · Exp 2_lacc 0-05.fcs · Exp 2_lacc 0-1 no 2.fcs · Exp 2_lacc 0-1.fcs · Exp 2_lacc HK 0-05.fcs · Exp 2_lacc HK 0-1 no2.fcs · Exp 2_lacc HK 0-1.fcs · laccase HK_0-05 no1.fcs · laccase HK_0-05 no2.fcs · laccase HK_0-05 no3.fcs · laccase HK_0-1 no 2.fcs · laccase HK_0-1 no 3.fcs · laccase HK_0-1 no1.fcs · laccase_0-01.fcs · laccase_0-05 no1.fcs · laccase_0-05 no2.fcs · laccase_0-05 no3.fcs · laccase_0-05.fcs · laccase_0-1 no 2.fcs · laccase_0-1 no 3.fcs · laccase_0-1 no1.fcs · laccase_0-1.fcs · laccase_001_0-01.fcs · laccase_001_0-05.fcs · laccase_001_0-1.fcs · laccase_002_0-01.fcs · laccase_002_0-05.fcs · laccase_002_0-1.fcs |
| · KYA | 6 hour_KYA 10.fcs · 6 hour_KYA 25.fcs · 6 hour_KYA 50.fcs · 6h_KYA 10.fcs · 6h_KYA 25.fcs · KYA KYN_KYA 10.fcs · KYA KYN_KYA 25.fcs · KYA_10.fcs · KYA_25.fcs · Kynurenic Acid_10.fcs · Kynurenic Acid_25.fcs · Kynurenic acid new_10 microM.fcs · Kynurenic acid new_25 microM.fcs |
| · L-KYN | 6 hour_LKYN 10.fcs · 6 hour_LKYN 25.fcs · 6 hour_LKYN 50.fcs · 6h_LKyn 10.fcs · 6h_LKyn 25.fcs · KYA KYN_KYN 10.fcs · KYA KYN_KYN 25.fcs · LKYN_10.fcs · LKYN_25.fcs · LKynurenine_10 microM.fcs · LKynurenine_10.fcs · LKynurenine_25 microM.fcs · LKynurenine_25.fcs |
| Doses | |
|---|---|
| · 0.01 µM | laccase_0-01.fcs · laccase_001_0-01.fcs · laccase_002_0-01.fcs |
| · 0.05 µM | Exp 1_Laccase 0-05.fcs · Exp 1_laccase HK 0-05.fcs · Exp 2_lacc 0-05.fcs · Exp 2_lacc HK 0-05.fcs · laccase HK_0-05 no1.fcs · laccase HK_0-05 no2.fcs · laccase HK_0-05 no3.fcs · laccase_0-05 no1.fcs · laccase_0-05 no2.fcs · laccase_0-05 no3.fcs · laccase_0-05.fcs · laccase_001_0-05.fcs · laccase_002_0-05.fcs |
| · 0.1 µM | 6 hour_laccase 0-1 UperML.fcs · Exp 1_laccase HK 0-1.fcs · Exp 2_lacc 0-1 no 2.fcs · Exp 2_lacc 0-1.fcs · Exp 2_lacc HK 0-1 no2.fcs · Exp 2_lacc HK 0-1.fcs · laccase HK_0-1 no 2.fcs · laccase HK_0-1 no 3.fcs · laccase HK_0-1 no1.fcs · laccase_0-1 no 2.fcs · laccase_0-1 no 3.fcs · laccase_0-1 no1.fcs · laccase_0-1.fcs · laccase_001_0-1.fcs · laccase_002_0-1.fcs |
| · 10 µM | 6 hour_CA 10.fcs · 6 hour_DMSO 10.fcs · 6 hour_KYA 10.fcs · 6 hour_LKYN 10.fcs · 6h_CA new 10.fcs · 6h_DMSO 10.fcs · 6h_KYA 10.fcs · 6h_LKyn 10.fcs · CA_10 microM.fcs · CA_10.fcs · DMSO_10 microM.fcs · DMSO_10.fcs · DMSO_10.fcs-(1) · DMSO_10.fcs-(2) · Exp 1_D10.fcs · Exp 2_D10.fcs · KYA KYN_KYA 10.fcs · KYA KYN_KYN 10.fcs · KYA_10.fcs · Kynurenic Acid_10.fcs · Kynurenic acid new_10 microM.fcs · LKYN_10.fcs · LKynurenine_10 microM.fcs · LKynurenine_10.fcs · beta_CA 10 beta 10 no PMA.fcs · beta_CA 10 beta 10.fcs · beta_DMSO beta 10.fcs |
| · 25 µM | 6 hour_CA 25.fcs · 6 hour_DMSO 25.fcs · 6 hour_KYA 25.fcs · 6 hour_LKYN 25.fcs · 6h_CA new 25.fcs · 6h_DMSO 25.fcs · 6h_KYA 25.fcs · 6h_LKyn 25.fcs · CA_25 microM.fcs · CA_25.fcs · CA_25.fcs-(1) · DMSO_25 microM.fcs · DMSO_25.fcs · DMSO_25.fcs-(1) · DMSO_25.fcs-(2) · Exp 1_D25.fcs · Exp 2_D25.fcs · KYA KYN_KYA 25.fcs · KYA KYN_KYN 25.fcs · KYA_25.fcs · Kynurenic Acid_25.fcs · Kynurenic acid new_25 microM.fcs · LKYN_25.fcs · LKynurenine_25 microM.fcs · LKynurenine_25.fcs · beta_CA 25 beta 10 no PMA.fcs · beta_CA 25 beta 10.fcs |
| · 50 µM | 6 hour_CA 50.fcs · 6 hour_DMSO 50.fcs · 6 hour_KYA 50.fcs · 6 hour_LKYN 50.fcs |
| · Other | DMSO_5.fcs · DMSO_5.fcs-(1) · DMSO_none.fcs · Exp 2_D5.fcs · beta_DMSO no PMA.fcs |
| Sample Type | |
|---|---|
| · Heat Killed Enzyme | Exp 1_laccase HK 0-05.fcs · Exp 1_laccase HK 0-1.fcs · Exp 2_lacc HK 0-05.fcs · Exp 2_lacc HK 0-1 no2.fcs · Exp 2_lacc HK 0-1.fcs · laccase HK_0-05 no1.fcs · laccase HK_0-05 no2.fcs · laccase HK_0-05 no3.fcs · laccase HK_0-1 no 2.fcs · laccase HK_0-1 no 3.fcs · laccase HK_0-1 no1.fcs |
| · Active Enyzme | 6 hour_laccase 0-1 UperML.fcs · Exp 2_lacc 0-05.fcs · Exp 2_lacc 0-1 no 2.fcs · Exp 2_lacc 0-1.fcs · laccase_0-01.fcs · laccase_0-05 no1.fcs · laccase_0-05 no2.fcs · laccase_0-05 no3.fcs · laccase_0-05.fcs · laccase_0-1 no 2.fcs · laccase_0-1 no 3.fcs · laccase_0-1 no1.fcs · laccase_0-1.fcs · laccase_001_0-01.fcs · laccase_001_0-05.fcs · laccase_001_0-1.fcs · laccase_002_0-01.fcs · laccase_002_0-05.fcs · laccase_002_0-1.fcs |
| · Other | 6 hour_CA 10.fcs · 6 hour_CA 25.fcs · 6 hour_CA 50.fcs · 6 hour_DMSO 10.fcs · 6 hour_DMSO 25.fcs · 6 hour_DMSO 50.fcs · 6 hour_KYA 10.fcs · 6 hour_KYA 25.fcs · 6 hour_KYA 50.fcs · 6 hour_LKYN 10.fcs · 6 hour_LKYN 25.fcs · 6 hour_LKYN 50.fcs · 6h_CA new 10.fcs · 6h_CA new 25.fcs · 6h_DMSO 10.fcs · 6h_DMSO 25.fcs · 6h_KYA 10.fcs · 6h_KYA 25.fcs · 6h_LKyn 10.fcs · 6h_LKyn 25.fcs · CA_10 microM.fcs · CA_10.fcs · CA_25 microM.fcs · CA_25.fcs · CA_25.fcs-(1) · DMSO_10 microM.fcs · DMSO_10.fcs · DMSO_10.fcs-(1) · DMSO_10.fcs-(2) · DMSO_25 microM.fcs · DMSO_25.fcs · DMSO_25.fcs-(1) · DMSO_25.fcs-(2) · DMSO_5.fcs · DMSO_5.fcs-(1) · DMSO_none.fcs · Exp 1_D10.fcs · Exp 1_D25.fcs · Exp 1_Laccase 0-05.fcs · Exp 2_D10.fcs · Exp 2_D25.fcs · Exp 2_D5.fcs · KYA KYN_KYA 10.fcs · KYA KYN_KYA 25.fcs · KYA KYN_KYN 10.fcs · KYA KYN_KYN 25.fcs · KYA_10.fcs · KYA_25.fcs · Kynurenic Acid_10.fcs · Kynurenic Acid_25.fcs · Kynurenic acid new_10 microM.fcs · Kynurenic acid new_25 microM.fcs · LKYN_10.fcs · LKYN_25.fcs · LKynurenine_10 microM.fcs · LKynurenine_10.fcs · LKynurenine_25 microM.fcs · LKynurenine_25.fcs · beta_CA 10 beta 10 no PMA.fcs · beta_CA 10 beta 10.fcs · beta_CA 25 beta 10 no PMA.fcs · beta_CA 25 beta 10.fcs · beta_DMSO beta 10.fcs · beta_DMSO no PMA.fcs |
