Experiment Overview

Repository ID: FR-FCM-ZZAK Experiment name: TNF-alpha and eosinophil apoptosis MIFlowCyt score: 91.00%
Primary researcher: Pinja Ilmarinen PI/manager: Hannu Kankaanranta Uploaded by: Pinja Ilmarinen
Experiment dates: 2013-09-16 - 2013-11-20 Dataset uploaded: Mar 2014 Last updated: Mar 2014
Keywords: [APOPTOSIS] Manuscripts: [24587316] [PMC3938678] Plosonelogo
Organizations: University of Tampere, School of Medicine / Immunopharmacology, Tampere, (Finland)
Purpose: The purpose of the experiment was to examine the effect of TNF-alpha on eosinophil longevity and which receptor of TNF-alpha is mediating the effect.
Conclusion: Our results suggest that TNF-alpha delays human eosinophil apoptosis via TNF-receptor 1.
Comments: Percentages of viable cells (Annexin-negative, PI-negative; lower left gate) are reported below each plot. Percentage of apoptotic cells may be calculated by summing upper left and upper right gates (Annexin V-positive population).
Funding: This study was supported by grants obtained from Tampere Tuberculosis Foundation (Tampere, Finland), the Finnish Anti-Tuberculosis Association Foundation (Helsinki, Finland), and the Competitive Research Funding of Seinäjoki Central Hospital and Tampere University Hospital (Grants VTR15, EVO1115, 9N023, 9M059, 9K048 and 9H031). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of manuscript.
Quality control: We used IL-5-treated cells as a positive control for cell survival.

Experiment variables

Sample Type
· IgG1 isotype control 10 µg/ml !TNF2598.002
· anti-TNFRI 10 µg/ml !TNF2598.012
· anti-TNFRI 10 µg/ml + TNF-alpha 10 ng/ml !TNF2598.020
· anti-TNFRII 10 µg/ml !TNF2598.022
· anti-TNFRII 10 µg/ml + TNF-alpha 10 ng/ml !TNF2598.029
· control for IL-5 !TNF2598.041
· IL-5 10 pM !TNF2598.043
· IgG1 isotype control 10 µg/ml + TNF-alpha 10 ng/ml !TNF2598.009

· 40 h !TNF2598.002 · !TNF2598.009 · !TNF2598.012 · !TNF2598.020 · !TNF2598.022 · !TNF2598.029 · !TNF2598.041 · !TNF2598.043

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