Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
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Experiment Overview

Repository ID: FR-FCM-ZZBD Experiment name: Effects of CA on Adult Naive T cells MIFlowCyt score: 79.57%
Primary researcher: Margaret Lowe PI/manager: Margaret Lowe Uploaded by: Margaret Lowe
Experiment dates: 2009-10-28 - 2009-11-12 Dataset uploaded: Apr 2014 Last updated: Aug 2014
Keywords: [human] [IL17] [IL22] [adult] [CD4 T cell] Manuscripts: [24498387] [PMC3912126] Plosonelogo
Organizations: University of California, San Francisco, San Francisco, CA (United States)
Purpose: To determine the effects of CA on human naive CD4 T cell differentiation
Conclusion: CA increases IL-22 more than HAA or DMSO control in human naive T cells
Comments: See Figure 5B in Lowe et al PLOS One 2014.
Funding: Not disclosed
Quality control: None


Experiment variables

Doses
· 10 IL22_CA 10 96 well.fcs · IL22_DMSO 10 96 well.fcs · IL22_HAA 10 96 well.fcs
· 25 DonorA CA cytokines_25 + cytokines.fcs · DonorA DMSO cytokines_25 + cytokines.fcs · DonorA haa cytokines_25 + cytokines.fcs · DonorB CA cytokines_25 + cytokines.fcs · DonorB DMSO cytokines_25 + cytokines.fcs · DonorB HAA cytokines_25 + cytokines.fcs · Donor C DMSO_25 + cytokines.fcs · Donor C HAA_25 + cytokines.fcs · Donor C CA_25 + cytokines.fcs

Conditions
· DMSO IL22_DMSO 10 96 well.fcs · DonorA DMSO cytokines_25 + cytokines.fcs · DonorB DMSO cytokines_25 + cytokines.fcs · Donor C DMSO_25 + cytokines.fcs
· HAA IL22_HAA 10 96 well.fcs · DonorA haa cytokines_25 + cytokines.fcs · DonorB HAA cytokines_25 + cytokines.fcs · Donor C HAA_25 + cytokines.fcs
· CA IL22_CA 10 96 well.fcs · DonorA CA cytokines_25 + cytokines.fcs · DonorB CA cytokines_25 + cytokines.fcs · Donor C CA_25 + cytokines.fcs

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