Experiment Overview
| Repository ID: | FR-FCM-ZZBF | Experiment name: | Expression of activation and inhibition markers on PMNs incubated with M. canettii and M. bovis BCG | MIFlowCyt score: | 95.12% |
| Primary researcher: | Mariana Orozco Uribe | PI/manager: | Mariana Orozco Uribe | Uploaded by: | Mariana Orozco Uribe |
| Experiment dates: | 2013-05-23 - 2013-05-24 | Dataset uploaded: | Apr 2014 | Last updated: | May 2014 |
| Keywords: | [Innate Immune Response] [Activation markers] [human neutrophils] [PD/PD-L1] [Mycobacteria] | Manuscripts: | |||
| Organizations: |
Centro de Investigación y Estudios Avanzados (Cinvestav) del IPN, Biología Celular, México, Distrito Federal (México)
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| Purpose: | The purpose of this experiment was to assess the expression of activation (CD16b, CD11b and CD66b) and inhibition (PD-1 and PD-L1) markers on human neutrophils incubated with two different strains of mycobacteria, Mycobacterium canettii and M. bovis BCG; at early time points of 15, 30 and 60 minutes. | ||||
| Conclusion: | Neutrophils are capable of modulate the expression of activation and inhibitory molecules in response to low-virulent mycobacteria. | ||||
| Comments: | None | ||||
| Funding: | The author of this work was a scholarship holder of Science and Technology National Council (CONACyT) with the registration number 419706. | ||||
| Quality control: | Freshly isolated neutrophils, untreated neutrophils, unstained neutrophils and isotype-staining controls were used in this experiment. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Unstained | 15 BCG 01 NEG.fcs · 15 BCG NEG.fcs · 15 MC 01 NEG.fcs · 15 MC 1 NEG.fcs · 30 BCG 01 NEG.fcs · 30 BCG 1 NEG.fcs · 30 MC 01 NEG.fcs · 30 MC 1 NEG.fcs · 60 BCG 01 NEG.fcs · 60 BCG 1 NEG.fcs · 60 MC 01 NEG.fcs · 60 MC 1 NEG.fcs · T0 NEG.fcs |
| · Activation | 15 BCG 01 ACT.fcs · 15 BCG 1 ACT.fcs · 15 MC 01 ACT.fcs · 15 MC 1 ACT.fcs · 15 MED ACT.fcs · 30 BCG 01 ACT.fcs · 30 BCG 1 ACT.fcs · 30 MC 01 ACT.fcs · 30 MC 1 ACT.fcs · 30 MED ACT.fcs · 60 BCG 01 ACT 2.fcs · 60 BCG 1 ACT.fcs · 60 MC 01 ACT.fcs · 60 MC 1 ACT.fcs · 60 MED ACT.fcs · T0 ACT.fcs |
| · Inhibition | 15 BCG 01 PD.fcs · 15 BCG 1 PD 2.fcs · 15 MC 01 PD.fcs · 15 MC 1 PD.fcs · 15 MED PD.fcs · 30 BCG 01 PD.fcs · 30 BCG 1 PD.fcs · 30 MC 01 PD.fcs · 30 MC 1 PD.fcs · 30 MED PD.fcs · 60 BCG 01 PD.fcs · 60 BCG 1 PD.fcs · 60 MC 01 PD.fcs · 60 MC 1 PD.fcs · 60 MED PD.fcs · T0 PD.fcs |
| · Isotype control | 15 MED ISO.fcs · 30 MED ISO.fcs · 60 MED ISO.fcs |
| Doses | |
|---|---|
| · Medium | 15 MED ACT.fcs · 15 MED ISO.fcs · 15 MED PD.fcs · 30 MED ACT.fcs · 30 MED ISO.fcs · 30 MED PD.fcs · 60 MED ACT.fcs · 60 MED ISO.fcs · 60 MED PD.fcs |
| · M. bovis BCG MOI 0.1 | 15 BCG 01 ACT.fcs · 15 BCG 01 NEG.fcs · 15 BCG 01 PD.fcs · 30 BCG 01 ACT.fcs · 30 BCG 01 NEG.fcs · 30 BCG 01 PD.fcs · 60 BCG 01 ACT 2.fcs · 60 BCG 01 NEG.fcs · 60 BCG 01 PD.fcs |
| · M. bovis BCG MOI 1 | 15 BCG 1 ACT.fcs · 15 BCG 1 PD 2.fcs · 15 BCG NEG.fcs · 30 BCG 1 ACT.fcs · 30 BCG 1 NEG.fcs · 30 BCG 1 PD.fcs · 60 BCG 1 ACT.fcs · 60 BCG 1 NEG.fcs · 60 BCG 1 PD.fcs |
| · M. canettii MOI 0.1 | 15 MC 01 ACT.fcs · 15 MC 01 NEG.fcs · 15 MC 01 PD.fcs · 30 MC 01 ACT.fcs · 30 MC 01 NEG.fcs · 30 MC 01 PD.fcs · 60 MC 01 ACT.fcs · 60 MC 01 NEG.fcs · 60 MC 01 PD.fcs |
| · M. canettii MOI 1 | 15 MC 1 ACT.fcs · 15 MC 1 NEG.fcs · 15 MC 1 PD.fcs · 30 MC 1 ACT.fcs · 30 MC 1 NEG.fcs · 30 MC 1 PD.fcs · 60 MC 1 ACT.fcs · 60 MC 1 NEG.fcs · 60 MC 1 PD.fcs |
| Timepoints | |
|---|---|
| · Time zero | T0 ACT.fcs · T0 NEG.fcs · T0 PD.fcs |
| · 15 minutes | 15 BCG 01 ACT.fcs · 15 BCG 01 NEG.fcs · 15 BCG 01 PD.fcs · 15 BCG 1 ACT.fcs · 15 BCG 1 PD 2.fcs · 15 BCG NEG.fcs · 15 MC 01 ACT.fcs · 15 MC 01 NEG.fcs · 15 MC 01 PD.fcs · 15 MC 1 ACT.fcs · 15 MC 1 NEG.fcs · 15 MC 1 PD.fcs · 15 MED ACT.fcs · 15 MED ISO.fcs · 15 MED PD.fcs |
| · 30 minutes | 30 BCG 01 ACT.fcs · 30 BCG 01 NEG.fcs · 30 BCG 01 PD.fcs · 30 BCG 1 ACT.fcs · 30 BCG 1 NEG.fcs · 30 BCG 1 PD.fcs · 30 MC 01 ACT.fcs · 30 MC 01 NEG.fcs · 30 MC 01 PD.fcs · 30 MC 1 ACT.fcs · 30 MC 1 NEG.fcs · 30 MC 1 PD.fcs · 30 MED ACT.fcs · 30 MED ISO.fcs · 30 MED PD.fcs |
| · 60 minutes | 60 BCG 01 ACT 2.fcs · 60 BCG 01 NEG.fcs · 60 BCG 01 PD.fcs · 60 BCG 1 ACT.fcs · 60 BCG 1 NEG.fcs · 60 BCG 1 PD.fcs · 60 MC 01 ACT.fcs · 60 MC 01 NEG.fcs · 60 MC 01 PD.fcs · 60 MC 1 ACT.fcs · 60 MC 1 NEG.fcs · 60 MC 1 PD.fcs · 60 MED ACT.fcs · 60 MED ISO.fcs · 60 MED PD.fcs |
