Experiment Overview
| Repository ID: | FR-FCM-ZZBG | Experiment name: | Exposure of PBMCs to CA increases IL22 production by CD4 | MIFlowCyt score: | 84.57% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2009-09-22 - 2010-04-08 | Dataset uploaded: | Apr 2014 | Last updated: | Aug 2014 |
| Keywords: | [IL17] [IL22] [human PBMCs] [stimulated] | Manuscripts: | [24498387] [PMC3912126] |
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| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
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| Purpose: | To determine the effects of CA exposure of PBMCs in regards to IL-22 production | ||||
| Conclusion: | CA increases IL-22 in human CD4 T cells | ||||
| Comments: | See Figure 5A of Lowe et al PLOS One 2014. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 0 µM | Donor 1 CA_0.fcs · Donor 1 DMSO_0.fcs |
| · 10 µM | Donor 1 CA_10.fcs · Donor 1 DMSO_10.fcs · Donor 1 DMSO_10 microM.fcs · Donor 1 new CA_10 microM.fcs · Donor 5 DMSO_10 microM.fcs · Donor 5 new CA_10 microM.fcs · Donor 2 CA old_10.fcs · Donor 2 DMSO_10.fcs · Donor 3 CA old_10.fcs · Donor 3 DMSO_10.fcs · Donor 2 CA old_10.fcs-(1) · Donor 2 DMSO_10.fcs-(1) · Donor 4 CA old_10.fcs · Donor 4 DMSO_10.fcs |
| · 25 µM | Donor 1 CA_25.fcs · Donor 1 DMSO_25.fcs · Donor 1 DMSO_25 microM.fcs · Donor 1 new CA_25 microM.fcs · Donor 5 DMSO_25 microM.fcs · Donor 5 new CA_25 microM.fcs · Donor 2 CA old_25.fcs · Donor 2 DMSO_25.fcs · Donor 3 CA old_25.fcs · Donor 3 DMSO_25.fcs · Donor 2 CA old_25.fcs-(1) · Donor 2 DMSO_25.fcs-(1) · Donor 4 CA old_25.fcs · Donor 4 DMSO_25.fcs |
| · 50 µM | Donor 1 CA_50.fcs · Donor 1 DMSO_50.fcs · Donor 1 DMSO_50 microM.fcs · Donor 1 new CA_50 microM.fcs · Donor 5 DMSO_50 microM.fcs · Donor 5 new CA_50 microM.fcs · Donor 2 CA old_50.fcs · Donor 2 DMSO_50.fcs · Donor 3 CA old_50.fcs · Donor 3 DMSO_50.fcs · Donor 2 CA old_50.fcs-(1) · Donor 2 DMSO_50.fcs-(1) · Donor 4 CA old_50.fcs · Donor 4 DMSO_50.fcs |
| · 75 µM | Donor 1 CA_75.fcs · Donor 1 DMSO_75.fcs · Donor 1 DMSO_75 microM.fcs · Donor 1 new CA_75 microM.fcs · Donor 5 DMSO_75 microM.fcs · Donor 5 new CA_75 microM.fcs · Donor 2 CA old_75.fcs · Donor 2 DMSO_75.fcs · Donor 3 CA old_75.fcs · Donor 3 DMSO_75.fcs · Donor 2 CA old_75.fcs-(1) · Donor 2 DMSO_75.fcs-(1) · Donor 4 CA old_75.fcs · Donor 4 DMSO_75.fcs |
| · 100 µM | Donor 1 CA_100.fcs · Donor 1 DMSO_100.fcs · Donor 1 DMSO_100 microM.fcs · Donor 1 new CA_100 microM.fcs · Donor 5 DMSO_100 microM.fcs · Donor 5 new CA_100 microM.fcs · Donor 2 CA old_100.fcs · Donor 2 DMSO_100.fcs · Donor 3 CA old_100.fcs · Donor 3 DMSO_100.fcs · Donor 2 CA old_100.fcs-(1) · Donor 2 DMSO_100.fcs-(1) · Donor 4 CA old_100.fcs · Donor 4 DMSO_100.fcs |
| Conditions | |
|---|---|
| · DMSO | Donor 1 DMSO_0.fcs · Donor 1 DMSO_10.fcs · Donor 1 DMSO_100.fcs · Donor 1 DMSO_25.fcs · Donor 1 DMSO_50.fcs · Donor 1 DMSO_75.fcs · Donor 1 DMSO_10 microM.fcs · Donor 1 DMSO_100 microM.fcs · Donor 1 DMSO_25 microM.fcs · Donor 1 DMSO_50 microM.fcs · Donor 1 DMSO_75 microM.fcs · Donor 5 DMSO_10 microM.fcs · Donor 5 DMSO_100 microM.fcs · Donor 5 DMSO_25 microM.fcs · Donor 5 DMSO_50 microM.fcs · Donor 5 DMSO_75 microM.fcs · Donor 2 DMSO_10.fcs · Donor 2 DMSO_100.fcs · Donor 2 DMSO_25.fcs · Donor 2 DMSO_50.fcs · Donor 2 DMSO_75.fcs · Donor 3 DMSO_10.fcs · Donor 3 DMSO_100.fcs · Donor 3 DMSO_25.fcs · Donor 3 DMSO_50.fcs · Donor 3 DMSO_75.fcs · Donor 2 DMSO_10.fcs-(1) · Donor 2 DMSO_100.fcs-(1) · Donor 2 DMSO_25.fcs-(1) · Donor 2 DMSO_50.fcs-(1) · Donor 2 DMSO_75.fcs-(1) · Donor 4 DMSO_10.fcs · Donor 4 DMSO_100.fcs · Donor 4 DMSO_25.fcs · Donor 4 DMSO_50.fcs · Donor 4 DMSO_75.fcs |
| · CA | Donor 1 CA_0.fcs · Donor 1 CA_10.fcs · Donor 1 CA_100.fcs · Donor 1 CA_25.fcs · Donor 1 CA_50.fcs · Donor 1 CA_75.fcs · Donor 1 new CA_10 microM.fcs · Donor 1 new CA_100 microM.fcs · Donor 1 new CA_25 microM.fcs · Donor 1 new CA_50 microM.fcs · Donor 1 new CA_75 microM.fcs · Donor 5 new CA_10 microM.fcs · Donor 5 new CA_100 microM.fcs · Donor 5 new CA_25 microM.fcs · Donor 5 new CA_50 microM.fcs · Donor 5 new CA_75 microM.fcs · Donor 2 CA old_10.fcs · Donor 2 CA old_100.fcs · Donor 2 CA old_25.fcs · Donor 2 CA old_50.fcs · Donor 2 CA old_75.fcs · Donor 3 CA old_10.fcs · Donor 3 CA old_100.fcs · Donor 3 CA old_25.fcs · Donor 3 CA old_50.fcs · Donor 3 CA old_75.fcs · Donor 2 CA old_10.fcs-(1) · Donor 2 CA old_100.fcs-(1) · Donor 2 CA old_25.fcs-(1) · Donor 2 CA old_50.fcs-(1) · Donor 2 CA old_75.fcs-(1) · Donor 4 CA old_10.fcs · Donor 4 CA old_100.fcs · Donor 4 CA old_25.fcs · Donor 4 CA old_50.fcs · Donor 4 CA old_75.fcs |
