Experiment Overview
| Repository ID: | FR-FCM-ZZBJ | Experiment name: | HAA effects on IL-22 in the presence of an AHR antagonist | MIFlowCyt score: | 80.29% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2008-12-20 - 2009-02-25 | Dataset uploaded: | Apr 2014 | Last updated: | Aug 2014 |
| Keywords: | [IL-22] [human PBMCs] [AHR antagonist] [HAA] | Manuscripts: | [24498387] [PMC3912126] |
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| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
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| Purpose: | To test whether the IL-22 production observed in CD4 T cells stimulated in the presence of HAA is inhibited by an AHR antagonist. | ||||
| Conclusion: | The increase in IL-22 caused by HAA is decreased by the addition of an AHR antagonist | ||||
| Comments: | See Fig. 1C and 1D of Lowe et al Plos One 2014. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · DMSO | DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs · DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs-(1) · DMSO no inh_DMSO 1,3a,10000_A04.fcs · DMSO no inh_DMSO 1,3a,10000_A04.fcs-(1) · DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs-(2) · DMSO AhR Inh_DMSO 1,3a,5000_B03.fcs · DMSO no inh_DMSO 1,3a,10000_A04.fcs-(2) · DMSO no inh_DMSO 1,3a,5000_A03.fcs · AhR Inhibitor DMSO,2f,FICZ_DMSO 50uM_E03.fcs · AhR Inhibitor DMSO,2f,FICZ_DMSO 50uM_E03.fcs-(1) · No Inhibitor DMSO,2f,FICZ_DMSO 50uM_F03.fcs · No Inhibitor DMSO,2f,FICZ_DMSO 50uM_F03.fcs-(1) |
| · HAA | HAA AhR Inh_50uM_D03.fcs · HAA AhR Inh_50uM_D03.fcs-(1) · HAA no inh_50uM_C03.fcs · HAA no inh_50uM_C03.fcs-(1) · HAA AhR Inh_50uM_D03.fcs-(2) · HAA AhR Inh_50uM_D03.fcs-(3) · HAA no inh_50uM_C03.fcs-(2) · HAA no inh_50uM_C03.fcs-(3) · AhR Inhibitor HAA,2f,PA_HAA 50uM_C03.fcs · AhR Inhibitor HAA,2f,PA_HAA 50uM_C03.fcs-(1) · No Inhibitor HAA,2f,PA_HAA 50uM_D03.fcs · No Inhibitor HAA,2f,PA_HAA 50uM_D03.fcs-(1) |
| · HKYN | HKyn AhR inh_50uM_D09.fcs · HKyn AhR inh_50uM_D09.fcs-(1) · HKyn no inh_50uM_C09.fcs · HKyn no inh_50uM_C09.fcs-(1) · HKyn AhR Inh_50uM_D09.fcs · HKyn AhR Inh_50uM_D09.fcs-(1) · HKyn no inh_50uM_C09.fcs-(2) · HKyn no inh_50uM_C09.fcs-(3) · No Inhibitor HKyn,2f,DAA_HKyn 50uM_B03.fcs · No Inhibitor HKyn,2f,DAA_HKyn 50uM_B03.fcs-(1) · AhR Inhibitor HKyn,2f,DAA_HKyn 50uM_A03.fcs · AhR Inhibitor HKyn,2f,DAA_HKyn 50uM_A03.fcs-(1) |
| Sample Type | |
|---|---|
| · With AHR antagonist | DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs · DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs-(1) · HAA AhR Inh_50uM_D03.fcs · HAA AhR Inh_50uM_D03.fcs-(1) · HKyn AhR inh_50uM_D09.fcs · HKyn AhR inh_50uM_D09.fcs-(1) · DMSO AhR Inh_DMSO 1,3a,10000_B04.fcs-(2) · DMSO AhR Inh_DMSO 1,3a,5000_B03.fcs · HAA AhR Inh_50uM_D03.fcs-(2) · HAA AhR Inh_50uM_D03.fcs-(3) · HKyn AhR Inh_50uM_D09.fcs · HKyn AhR Inh_50uM_D09.fcs-(1) · AhR Inhibitor DMSO,2f,FICZ_DMSO 50uM_E03.fcs · AhR Inhibitor DMSO,2f,FICZ_DMSO 50uM_E03.fcs-(1) · AhR Inhibitor HAA,2f,PA_HAA 50uM_C03.fcs · AhR Inhibitor HAA,2f,PA_HAA 50uM_C03.fcs-(1) · AhR Inhibitor HKyn,2f,DAA_HKyn 50uM_A03.fcs · AhR Inhibitor HKyn,2f,DAA_HKyn 50uM_A03.fcs-(1) |
| · Comparator | DMSO no inh_DMSO 1,3a,10000_A04.fcs · DMSO no inh_DMSO 1,3a,10000_A04.fcs-(1) · HAA no inh_50uM_C03.fcs · HAA no inh_50uM_C03.fcs-(1) · HKyn no inh_50uM_C09.fcs · HKyn no inh_50uM_C09.fcs-(1) · DMSO no inh_DMSO 1,3a,10000_A04.fcs-(2) · DMSO no inh_DMSO 1,3a,5000_A03.fcs · HAA no inh_50uM_C03.fcs-(2) · HAA no inh_50uM_C03.fcs-(3) · HKyn no inh_50uM_C09.fcs-(2) · HKyn no inh_50uM_C09.fcs-(3) · No Inhibitor DMSO,2f,FICZ_DMSO 50uM_F03.fcs · No Inhibitor DMSO,2f,FICZ_DMSO 50uM_F03.fcs-(1) · No Inhibitor HAA,2f,PA_HAA 50uM_D03.fcs · No Inhibitor HAA,2f,PA_HAA 50uM_D03.fcs-(1) · No Inhibitor HKyn,2f,DAA_HKyn 50uM_B03.fcs · No Inhibitor HKyn,2f,DAA_HKyn 50uM_B03.fcs-(1) |
