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Experiment Overview

Repository ID: FR-FCM-ZZBK Experiment name: Effects of HAA on Cytokine Production by CD4 T cells MIFlowCyt score: 87.58%
Primary researcher: Margaret Lowe PI/manager: Margaret Lowe Uploaded by: Margaret Lowe
Experiment dates: 2008-07-21 - 2009-10-01 Dataset uploaded: Apr 2014 Last updated: Aug 2014
Keywords: [human] [IL17] [IL22] [HAA] [CD4 T cells] [IFNg] Manuscripts: [24498387] [PMC3912126] Plosonelogo
Organizations: University of California, San Francisco, San Francisco, CA (United States)
Purpose: To test the effects of HAA and other tryptophan metabolites on cytokine production by CD4 T cells.
Conclusion: HAA increases IL-22 while downstream metabolites do not.
Comments: See Figs 1A&B and 2 A&B of Lowe et al Plos One 2014
Funding: Not disclosed
Quality control: None


Experiment variables

Doses
· 10 µM DMSO ctrl Donor 3_10uM_Sample1.fcs · DMSO ctrl Donor 3_10uM_Sample2.fcs · DMSO ctrl Donor 4_10uM_Sample1.fcs · DMSO ctrl Donor 4_10uM_Sample2.fcs · HAA Donor 3_10uM_Sample1.fcs · HAA Donor 3_10uM_Sample2.fcs · HAA Donor 4_10uM_Sample1.fcs · HAA Donor 4_10uM_Sample2.fcs · HKyn Donor 3_10uM_Sample1.fcs · Hkyn Donor 3_10uM_Sample2.fcs · Hkyn Donor 4_10uM_Sample1.fcs · Hkyn Donor 4_10uM_Sample2.fcs · PA Donor 3_10uM_Sample1.fcs · PA Donor 3_10uM_Sample2.fcs · PA Donor 4_10uM_Sample1.fcs · PA Donor 4_10uM_Sample2.fcs · DMSO_10 microM.fcs · DMSO_10 microM.fcs-(1) · HAA_10 microM.fcs · HAA_10 microM.fcs-(1) · HKyn_10 microM.fcs · HKyn_10 microM.fcs-(1) · PA_10 microM.fcs · PA_10 microM.fcs-(1) · QA_10 microM.fcs · QA_10 microM.fcs-(1) · Donor 3 DMSO_10.fcs · Donor 3 HAA_10.fcs · Donor 3 Hkyn_10.fcs · Donor 3 PA_10.fcs · Donor 3 QA_10.fcs · HAA 10 uM_D02.fcs · HAA 10 uM_D02.fcs-(1) · HAA 10 uM_D08.fcs · HAA 10 uM_D08.fcs-(1) · HKYN 10 uM_H02.fcs · HKYN 10 uM_H02.fcs-(1) · HKYN 10 uM_H08.fcs · HKYN 10 uM_H08.fcs-(1) · PA 10 uM_D02.fcs · PA 10 uM_D02.fcs-(1) · PA 10 uM_D08.fcs · PA 10 uM_D08.fcs-(1) · Donor 6 DMSO_10.fcs · Donor 6 HAA_10.fcs · Donor 6 Hkyn_10.fcs · Donor 6 PA_10.fcs · Donor 6 QA_10.fcs · HAA_10uM_D12.fcs · HAA_10uM_D12.fcs-(1) · HydroyKynurenine_10uM_F12.fcs · HydroyKynurenine_10uM_F12.fcs-(1) · Picolinic Acid_10uM_E12.fcs · Picolinic Acid_10uM_E12.fcs-(1) · DMSO 1,3a,50000_A05.fcs · DMSO 1,3a,50000_A05.fcs-(1) · DMSO 1,3a,50000_A05.fcs-(2) · DMSO 1,3a,50000_A05.fcs-(3)
· 25 µM DMSO ctrl Donor 3_25uM_Sample1.fcs · DMSO ctrl Donor 3_25uM_Sample2.fcs · DMSO ctrl Donor 4_25uM_Sample1.fcs · DMSO ctrl Donor 4_25uM_Sample2.fcs · HAA Donor 3_25uM_Sample1.fcs · HAA Donor 3_25uM_Sample2.fcs · HAA Donor 4_25uM_Sample1.fcs · HAA Donor 4_25uM_Sample2.fcs · HKyn Donor 3_25uM_Sample1.fcs · Hkyn Donor 3_25uM_Sample2.fcs · Hkyn Donor 4_25uM_Sample1.fcs · Hkyn Donor 4_25uM_Sample2.fcs · PA Donor 3_25uM_Sample1.fcs · PA Donor 3_25uM_Sample2.fcs · PA Donor 4_25uM_Sample1.fcs · PA Donor 4_25uM_Sample2.fcs · Donor 3 DMSO_25.fcs · Donor 3 HAA_25.fcs · Donor 3 Hkyn_25.fcs · Donor 3 PA_25.fcs · Donor 3 QA_25.fcs · HAA 25 uM_D03.fcs · HAA 25 uM_D03.fcs-(1) · HAA 25 uM_D09.fcs · HAA 25 uM_D09.fcs-(1) · HKYN 25 uM_H03.fcs · HKYN 25 uM_H03.fcs-(1) · HKYN 25 uM_H09.fcs · HKYN 25 uM_H09.fcs-(1) · PA 25 uM_D03.fcs · PA 25 uM_D03.fcs-(1) · PA 25 uM_D09.fcs · PA 25 uM_D09.fcs-(1) · Donor 6 DMSO_25.fcs · Donor 6 HAA_25.fcs · Donor 6 Hkyn_25.fcs · Donor 6 PA_25.fcs · Donor 6 QA_25.fcs · HAA_25uM_D11.fcs · HAA_25uM_D11.fcs-(1) · HydroyKynurenine_25uM_F11.fcs · HydroyKynurenine_25uM_F11.fcs-(1) · Picolinic Acid_25uM_E11.fcs · Picolinic Acid_25uM_E11.fcs-(1) · DMSO 25uM_F02.fcs · DMSO 25uM_F02.fcs-(1) · HAA 25uM_D02.fcs · HAA 25uM_D02.fcs-(1) · HKyn 25uM_B02.fcs · HKyn 25uM_B02.fcs-(1) · PA 25uM_D08.fcs · PA 25uM_D08.fcs-(1) · HAA 25uM_C02.fcs · HAA_25uM_C02.fcs · HKyn 25uM_C08.fcs · HKyn_25uM_C08.fcs · PA 25uM_E02.fcs · PA_25uM_E02.fcs · QA 25uM_E08.fcs · QA_25uM_E08.fcs · HAA 25uM_C02.fcs-(1) · HAA 25uM_C02.fcs-(2) · HKyn 25uM_C08.fcs-(1) · HKyn 25uM_C08.fcs-(2) · PA 25uM_E02.fcs-(1) · PA 25uM_E02.fcs-(2) · QA 25uM_E08.fcs-(1) · QA 25uM_E08.fcs-(2)
· 50 µM DMSO ctrl Donor 3_50uM_Sample1.fcs · DMSO ctrl Donor 3_50uM_Sample2.fcs · DMSO ctrl Donor 4_50uM_Sample1.fcs · DMSO ctrl Donor 4_50uM_Sample2.fcs · HAA Donor 3_50uM_Sample1.fcs · HAA Donor 3_50uM_Sample2.fcs · HAA Donor 4_50uM_Sample1.fcs · HAA Donor 4_50uM_Sample2.fcs · HKyn Donor 3_50uM_Sample1.fcs · Hkyn Donor 3_50uM_Sample2.fcs · Hkyn Donor 4_50uM_Sample1.fcs · Hkyn Donor 4_50uM_Sample2.fcs · PA Donor 3_50uM_Sample1.fcs · PA Donor 3_50uM_Sample2.fcs · PA Donor 4_50uM_Sample1.fcs · PA Donor 4_50uM_Sample2.fcs · DMSO_50 microM.fcs · DMSO_50 microM.fcs-(1) · HAA_50 microM.fcs · HAA_50 microM.fcs-(1) · HKyn_50 microM.fcs · HKyn_50 microM.fcs-(1) · PA_50 microM.fcs · PA_50 microM.fcs-(1) · QA_50 microM.fcs · QA_50 microM.fcs-(1) · Donor 3 DMSO_50.fcs · Donor 3 HAA_50.fcs · Donor 3 Hkyn_50.fcs · Donor 3 PA_50.fcs · HAA 50 uM_D04.fcs · HAA 50 uM_D04.fcs-(1) · HAA 50 uM_D10.fcs · HAA 50 uM_D10.fcs-(1) · HKYN 50 uM_H04.fcs · HKYN 50 uM_H04.fcs-(1) · HKYN 50 uM_H10.fcs · HKYN 50 uM_H10.fcs-(1) · PA 50 uM_D04.fcs · PA 50 uM_D04.fcs-(1) · PA 50 uM_D10.fcs · PA 50 uM_D10.fcs-(1) · Donor 6 DMSO_50.fcs · Donor 6 HAA_50.fcs · Donor 6 Hkyn_50.fcs · Donor 6 PA_50.fcs · Donor 6 QA_50.fcs · DMSO_1,3a,10000_D04.fcs · DMSO_1,3a,10000_D04.fcs-(1) · HAA_50uM_D10.fcs · HAA_50uM_D10.fcs-(1) · HydroyKynurenine_50uM_F10.fcs · HydroyKynurenine_50uM_F10.fcs-(1) · Picolinic Acid_50uM_E10.fcs · Picolinic Acid_50uM_E10.fcs-(1) · DMSO 50uM_F03.fcs · DMSO 50uM_F03.fcs-(1) · HAA 50uM_D03.fcs · HAA 50uM_D03.fcs-(1) · HKyn 50uM_B03.fcs · HKyn 50uM_B03.fcs-(1) · PA 50uM_D09.fcs · PA 50uM_D09.fcs-(1) · DMSO 1,3a,10000_A04.fcs · DMSO 1,3a,10000_A04.fcs-(1) · HAA 50uM_C03.fcs · HAA_50uM_C03.fcs · HKyn 50uM_C09.fcs · HKyn_50uM_C09.fcs · PA 50uM_E03.fcs · PA_50uM_E03.fcs · QA 50uM_E09.fcs · QA_50uM_E09.fcs · DMSO 1,3a,10000_A04.fcs-(2) · DMSO 1,3a,10000_A04.fcs-(3) · HAA 50uM_C03.fcs-(1) · HAA 50uM_C03.fcs-(2) · HKyn 50uM_C09.fcs-(1) · HKyn 50uM_C09.fcs-(2) · PA 50uM_E03.fcs-(1) · PA 50uM_E03.fcs-(2) · QA 50uM_E09.fcs-(1) · QA 50uM_E09.fcs-(2)
· 75 µM DMSO ctrl Donor 3_75uM_Sample1.fcs · DMSO ctrl Donor 3_75uM_Sample2.fcs · DMSO ctrl Donor 4_75uM_Sample1.fcs · DMSO ctrl Donor 4_75uM_Sample2.fcs · HAA Donor 3_75uM_Sample1.fcs · HAA Donor 3_75uM_Sample2.fcs · HAA Donor 4_75uM_Sample1.fcs · HAA Donor 4_75uM_Sample2.fcs · HKyn Donor 3_75uM_Sample1.fcs · Hkyn Donor 3_75uM_Sample2.fcs · Hkyn Donor 4_75uM_Sample1.fcs · Hkyn Donor 4_75uM_Sample2.fcs · PA Donor 3_75uM_Sample1.fcs · PA Donor 3_75uM_Sample2.fcs · PA Donor 4_75uM_Sample1.fcs · PA Donor 4_75uM_Sample2.fcs · DMSO_75 microM.fcs · DMSO_75 microM.fcs-(1) · HAA_75 microM.fcs · HAA_75 microM.fcs-(1) · HKyn_75 microM.fcs · HKyn_75 microM.fcs-(1) · PA_75 microM.fcs · PA_75 microM.fcs-(1) · QA_75 microM.fcs · QA_75 microM.fcs-(1) · Donor 3 DMSO_75.fcs · Donor 3 HAA_75.fcs · Donor 3 Hkyn_75.fcs · Donor 3 PA_75.fcs · HAA 75 uM_D05.fcs · HAA 75 uM_D05.fcs-(1) · HAA 75 uM_D11.fcs · HAA 75 uM_D11.fcs-(1) · HKYN 75 uM_H05.fcs · HKYN 75 uM_H05.fcs-(1) · HKYN 75 uM_H11.fcs · HKYN 75 uM_H11.fcs-(1) · PA 75 uM_D05.fcs · PA 75 uM_D05.fcs-(1) · PA 75 uM_D11.fcs · PA 75 uM_D11.fcs-(1) · Donor 6 DMSO_75.fcs · Donor 6 HAA_75.fcs · Donor 6 Hkyn_75.fcs · Donor 6 PA_75.fcs · Donor 6 QA_75.fcs · HAA_75uM_D09.fcs · HAA_75uM_D09.fcs-(1) · HydroyKynurenine_75uM_F09.fcs · HydroyKynurenine_75uM_F09.fcs-(1) · Picolinic Acid_75uM_E09.fcs · Picolinic Acid_75uM_E09.fcs-(1) · DMSO 75uM_F04.fcs · DMSO 75uM_F04.fcs-(1) · HAA 75uM_D04.fcs · HAA 75uM_D04.fcs-(1) · HKYN 75 uM_H11.fcs-(1).deidentify.temp · HKyn 75uM_B04.fcs · HKyn 75uM_B04.fcs-(1) · PA 75uM_D10.fcs · PA 75uM_D10.fcs-(1) · HAA 75uM_C04.fcs · HAA_75uM_C04.fcs · HKyn 75uM_C10.fcs · HKyn_75uM_C10.fcs · PA 75uM_E04.fcs · PA_75uM_E04.fcs · QA 75uM_E10.fcs · QA_75uM_E10.fcs · HAA 75uM_C04.fcs-(1) · HAA 75uM_C04.fcs-(2) · HKyn 75uM_C10.fcs-(1) · HKyn 75uM_C10.fcs-(2) · PA 75uM_E04.fcs-(1) · PA 75uM_E04.fcs-(2) · QA 75uM_E10.fcs-(1) · QA 75uM_E10.fcs-(2)
· 100 µM DMSO ctrl Donor 3_100uM_Sample1.fcs · DMSO ctrl Donor 3_100uM_Sample2.fcs · DMSO ctrl Donor 4 100uM_Sample1.fcs · DMSO ctrl Donor 4_100uM_Sample2.fcs · HAA Donor 3_100uM_Sample1.fcs · HAA Donor 3_100uM_Sample2.fcs · HAA Donor 4_100uM_Sample1.fcs · HAA Donor 4_100uM_Sample2.fcs · HKyn Donor 3_100uM_Sample1.fcs · Hkyn Donor 3_100uM_Sample2.fcs · Hkyn Donor 4_100uM_Sample1.fcs · Hkyn Donor 4_100uM_Sample2.fcs · PA Donor 3_100uM_Sample1.fcs · PA Donor 3_100uM_Sample2.fcs · PA Donor 4_100uM_Sample1.fcs · PA Donor 4_100uM_Sample2.fcs · DMSO_100 microM.fcs · DMSO_100 microM.fcs-(1) · HAA_100 microM.fcs · HAA_100 microM.fcs-(1) · HKyn_100 microM.fcs · HKyn_100 microM.fcs-(1) · PA_100 microM.fcs · PA_100 microM.fcs-(1) · QA_100 microM.fcs · QA_100 microM.fcs-(1) · Donor 3 DMSO_100.fcs · Donor 3 HAA_100.fcs · Donor 3 Hkyn_100.fcs · Donor 3 PA_100.fcs · HAA 100 uM_D06.fcs · HAA 100 uM_D06.fcs-(1) · HAA 100 uM_D12.fcs · HAA 100 uM_D12.fcs-(1) · HKYN 100 uM_H06.fcs · HKYN 100 uM_H06.fcs-(1) · HKYN 100 uM_H12.fcs · HKYN 100 uM_H12.fcs-(1) · PA 100 uM_D06.fcs · PA 100 uM_D06.fcs-(1) · PA 100 uM_D12.fcs · PA 100 uM_D12.fcs-(1) · Donor 6 DMSO_100.fcs · Donor 6 HAA_100.fcs · Donor 6 Hkyn_100.fcs · Donor 6 PA_100.fcs · Donor 6 QA_100.fcs · DMSO_1,3a,5000_D03.fcs · DMSO_1,3a,5000_D03.fcs-(1) · HAA_100uM_D08.fcs · HAA_100uM_D08.fcs-(1) · HydroyKynurenine_100uM_F08.fcs · HydroyKynurenine_100uM_F08.fcs-(1) · Picolinic Acid_100uM_E08.fcs · Picolinic Acid_100uM_E08.fcs-(1) · DMSO 100uM_F05.fcs · DMSO 100uM_F05.fcs-(1) · HAA 100uM_D05.fcs · HAA 100uM_D05.fcs-(1) · HKyn 100uM_B05.fcs · HKyn 100uM_B05.fcs-(1) · PA 100uM_D11.fcs · PA 100uM_D11.fcs-(1) · DMSO 1,3a,5000_A03.fcs · DMSO 1,3a5000_A03.fcs · HAA 100uM_C05.fcs · HAA_100uM_C05.fcs · HKyn 100uM_C11.fcs · HKyn_100uM_C11.fcs · PA 100uM_E05.fcs · PA_100uM_E05.fcs · QA 100uM_E11.fcs · QA_100uM_E11.fcs · DMSO 1,3a,5000_A03.fcs-(1) · DMSO 1,3a,5000_A03.fcs-(2) · HAA 100uM_C05.fcs-(1) · HAA 100uM_C05.fcs-(2) · HKyn 100uM_C11.fcs-(1) · HKyn 100uM_C11.fcs-(2) · PA 100uM_E05.fcs-(1) · PA 100uM_E05.fcs-(2) · QA 100uM_E11.fcs-(1) · QA 100uM_E11.fcs-(2)
· other (not depicted in figure) DMSO_10 microM+HAAOI.fcs · DMSO_10 microM+HAAOI.fcs-(1) · DMSO_20 microM.fcs · DMSO_20 microM.fcs-(1) · DMSO_30 microM.fcs · DMSO_30 microM.fcs-(1) · DMSO_5 microM.fcs · DMSO_50 microM+HAAOI.fcs · DMSO_50 microM+HAAOI.fcs-(1) · HAA_10 microM+HAAOI.fcs · HAA_10 microM+HAAOI.fcs-(1) · HAA_20 microM.fcs · HAA_20 microM.fcs-(1) · HAA_30 microM.fcs · HAA_30 microM.fcs-(1) · HAA_5 microM.fcs · HAA_5 microM.fcs-(1) · HAA_50 microM+HAAOI.fcs · HAA_50 microM+HAAOI.fcs-(1) · HKyn_10 microM+HAAOI.fcs · HKyn_10 microM+HAAOI.fcs-(1) · HKyn_20 microM.fcs · HKyn_20 microM.fcs-(1) · HKyn_30 microM.fcs · HKyn_30 microM.fcs-(1) · HKyn_5 microM.fcs · HKyn_5 microM.fcs-(1) · HKyn_50 microM+HAAOI.fcs · HKyn_50 microM+HAAOI.fcs-(1) · PA_10 microM+HAAOI.fcs · PA_10 microM+HAAOI.fcs-(1) · PA_20 microM.fcs · PA_20 microM.fcs-(1) · PA_30 microM.fcs · PA_30 microM.fcs-(1) · PA_5 microM.fcs · PA_5 microM.fcs-(1) · PA_50 microM+HAAOI.fcs · PA_50 microM+HAAOI.fcs-(1) · QA_10 microM+HAAOI.fcs · QA_10 microM+HAAOI.fcs-(1) · QA_20 microM.fcs · QA_20 microM.fcs-(1) · QA_30 microM.fcs · QA_30 microM.fcs-(1) · QA_5 microM.fcs · QA_5 microM.fcs-(1) · QA_50 microM+HAAOI.fcs · QA_50 microM+HAAOI.fcs-(1) · Donor 3 DMSO_0.fcs · Donor 3 HAA_0.fcs · Donor 3 Hkyn_0.fcs · Donor 3 PA_0.fcs · Donor 3 QA_0.fcs · Control 0 uM_1.fcs · Control 0 uM_2.fcs · Control 0 uM_3.fcs · Control 0 uM_4.fcs · Control 0 uM_5.fcs · Control 1 0 uM_H07.fcs · Control 2 0 uM_D07.fcs · Control 3 0 uM_H01.fcs · Control 4 0 uM_D01.fcs · Control 5 0 uM_D07.fcs · Control 6 0 uM_D01.fcs · Donor 6 DMSO_0.fcs · Donor 6 HAA_0.fcs · Donor 6 Hkyn_0.fcs · Donor 6 PA_0.fcs · Donor 6 QA_0.fcs · DMSO_1,3a,1000_D02.fcs · DMSO_1,3a,1000_D02.fcs-(1) · DMSO_1,3a,500_D01.fcs · DMSO_1,3a,500_D01.fcs-(1) · HAA_150uM_D07.fcs · HAA_150uM_D07.fcs-(1) · HAA_200uM_D06.fcs · HAA_200uM_D06.fcs-(1) · HAA_300uM_D05.fcs · HAA_300uM_D05.fcs-(1) · HydroyKynurenine_150uM_F07.fcs · HydroyKynurenine_150uM_F07.fcs-(1) · HydroyKynurenine_200uM_F06.fcs · HydroyKynurenine_200uM_F06.fcs-(1) · HydroyKynurenine_300uM_F05.fcs · HydroyKynurenine_300uM_F05.fcs-(1) · Picolinic Acid_150uM_E07.fcs · Picolinic Acid_150uM_E07.fcs-(1) · Picolinic Acid_200uM_E06.fcs · Picolinic Acid_200uM_E06.fcs-(1) · Picolinic Acid_300uM_E05.fcs · Picolinic Acid_300uM_E05.fcs-(1) · DMSO 0uM_F01.fcs · DMSO 0uM_F01.fcs-(1) · DMSO 150uM_F06.fcs · DMSO 150uM_F06.fcs-(1) · HAA 0uM_D01.fcs · HAA 0uM_D01.fcs-(1) · HAA 150uM_D06.fcs · HAA 150uM_D06.fcs-(1) · HKyn 150uM_B06.fcs · HKyn 150uM_B06.fcs-(1) · Hkyn 0_B01.fcs · Hkyn 0_B01.fcs-(1) · PA 0uM_D07.fcs · PA 0uM_D07.fcs-(1) · PA 150uM_D12.fcs · PA 150uM_D12.fcs-(1) · DMSO 1,3a,100000_A06.fcs · DMSO 1,3a,100000_A06.fcs-(1) · DMSO 1,3a,1000_A02.fcs · DMSO 1,3a,1000_A02.fcs-(1) · DMSO 1,3a,500_A01.fcs · DMSO 1,3a,500_A01.fcs-(1) · HAA 0uM_C01.fcs · HAA 150uM_C06.fcs · HAA_0uM_C01.fcs · HAA_150uM_C06.fcs · HKyn 0uM_C07.fcs · HKyn 150uM_C12.fcs · HKyn_0uM_C07.fcs · HKyn_150uM_C12.fcs · PA 0uM_E01.fcs · PA 150uM_E06.fcs · PA_0uM_E01.fcs · PA_150uM_E06.fcs · QA 0uM_E07.fcs · QA 150uM_E12.fcs · QA_0uM_E07.fcs · QA_150uM_E12.fcs · DMSO 1,3a,100000_A06.fcs · DMSO 1,3a,100000_A06.fcs-(2) · DMSO 1,3a,1000_A02.fcs-(2) · DMSO 1,3a,1000_A02.fcs-(3) · DMSO 1,3a,500_A01.fcs-(2) · DMSO 1,3a,500_A01.fcs-(3) · HAA 0uM_C01.fcs-(1) · HAA 0uM_C01.fcs-(2) · HAA 150uM_C06.fcs-(1) · HAA 150uM_C06.fcs-(2) · HKyn 0uM_C07.fcs-(1) · HKyn 0uM_C07.fcs-(2) · HKyn 150uM_C12.fcs-(1) · HKyn 150uM_C12.fcs-(2) · PA 0uM_E01.fcs-(1) · PA 0uM_E01.fcs-(2) · PA 150uM_E06.fcs-(1) · PA 150uM_E06.fcs-(2) · QA 0uM_E07.fcs-(1) · QA 0uM_E07.fcs-(2) · QA 150uM_E12.fcs-(1) · QA 150uM_E12.fcs-(2)

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