Experiment Overview
| Repository ID: | FR-FCM-ZZBS | Experiment name: | Cherax interleukin | MIFlowCyt score: | 96.33% |
| Primary researcher: | Oscar Vivanco | PI/manager: | Oscar Vivanco | Uploaded by: | Oscar Vivanco |
| Experiment dates: | 2014-02-01 - 2014-07-01 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
| Keywords: | [CD25] [inmune innate] [invertebrate] | Manuscripts: | |||
| Organizations: |
Facultad de Medicina, Bioquimica , Mexico, Distrito Federal (México)
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| Purpose: | Characterizing populations of Australian shrimp hemocytes Cherax quadricarinatus and CD25 positive populations | ||||
| Conclusion: | It was determined by flow cytometry cells Cherax quadricarinatus shrimp have two majority populations, one of granularity and small and a larger one with no granulomas. Both populations exhibit marking the alpha subunit of the interleukin-2 receptor (cd25), these results are consistent with microscopy data that help us to know the percentage ratio of such markers. Although lack detereminar sequences to determine if homology or analogy to CD25. | ||||
| Comments: | None | ||||
| Funding: | PAPIIT IN226211 | ||||
| Quality control: | A test on populations of 3 individuals males age 2 was performed, a "pool" of cells extracted with PFH 4% and anticoagulant at 4 ° C, incubations were at 37 ° C, the monoclonal antibody and the isotype is of the same is performed company (BioLegend). To determine the relative size of a population analysis of mouse lymphocytes were collected and compared between them. | ||||
Experiment variables
| Doses | |
|---|---|
| · 100 microlitros | Oscar V 2014-05-21.001.fcs · Oscar V 2014-05-21.002.fcs · Oscar V 2014-05-21.005.fcs |
| Timepoints | |
|---|---|
| · 25 minutes fixing | Oscar V 2014-05-21.001.fcs · Oscar V 2014-05-21.002.fcs · Oscar V 2014-05-21.005.fcs |
| Conditions | |
|---|---|
| · 25 minute incubation CD25 | Oscar V 2014-05-21.002.fcs |
| · 25 minute incubation isotype | Oscar V 2014-05-21.005.fcs |
| · 25 minutes PBS | Oscar V 2014-05-21.001.fcs |
| Individuals | |
|---|---|
| · males | Oscar V 2014-05-21.001.fcs · Oscar V 2014-05-21.002.fcs · Oscar V 2014-05-21.005.fcs |
| Sample Type | |
|---|---|
| · hemolymph | Oscar V 2014-05-21.001.fcs · Oscar V 2014-05-21.002.fcs · Oscar V 2014-05-21.005.fcs |
