Experiment Overview
| Repository ID: | FR-FCM-ZZBT | Experiment name: | RNA Flow Cytometry: Comparison of Fresh vs Cryopreserved HIV Infected Cells | MIFlowCyt score: | 98.00% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2012-06-26 - 2012-07-09 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
| Keywords: | [HIV] [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] | Manuscripts: | [23441230] [PMC3575505] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The Purpose of this experiment was to demonstrate that the RNA Flow cytometry can be applied to cryopreserved cells. | ||||
| Conclusion: | Comparable HIV Gag RNA positive populations were identified in both the fresh and cryopreserved samples, compared to their respective mock infected cells. | ||||
| Comments: | Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 3b | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cytometer was setup using BD Cytometer Setup and Tracking (CST) in FACSuite software. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Fresh Cultured Cells | Fresh_IIIB_001.fcs · Fresh_Mock_002.fcs |
| · Cryopreserved Cells | Frozen_IIIB_003.fcs · Frozen_Mock_004.fcs |
| Sample Type | |
|---|---|
| · HIV+ | Fresh_IIIB_001.fcs · Frozen_IIIB_003.fcs |
| · HIV- | Fresh_Mock_002.fcs · Frozen_Mock_004.fcs |
