Experiment Overview
| Repository ID: | FR-FCM-ZZC8 | Experiment name: | CD4 and INFg mRNA/Protein expression in stimulated PBMC | MIFlowCyt score: | 100.00% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2014-03-31 - 2014-04-01 | Dataset uploaded: | May 2014 | Last updated: | Aug 2014 |
| Keywords: | [PBMC] [CD4] [Gene expression] [stimulated] [RNA Flow cytometry] | Manuscripts: | [25123411] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose was to demonstrate the effect of a 5 hour PMA/Ionomycin stimulation on expression of IFNg mRNA, CD4 mRNA, CD4 protein, and IFNg protein in lymphocytes. | ||||
| Conclusion: | After a 5 hour PMA/Ionomycin treatment, expression of CD4 protein and mRNA is decreased, while both IFNg protein and mRNA is increased. | ||||
| Comments: | Figure 2A-F and supplemental: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cytometer setup using CST in FACSDiva software. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · PBMC | PBMC_Stim 5 hr no Blocker_003.fcs · PBMC_Unstim 5hr no Blocker_001.fcs |
| · PBMC no target probe added negative control | PBMC_NTC Stim 5hr no Blocker_004.fcs · PBMC_NTC Unstim 5hr no Blocker_002.fcs |
| Conditions | |
|---|---|
| · 5 hour PMA/Ionomycin | PBMC_NTC Stim 5hr no Blocker_004.fcs · PBMC_Stim 5 hr no Blocker_003.fcs |
| · 5 hour rest | PBMC_NTC Unstim 5hr no Blocker_002.fcs · PBMC_Unstim 5hr no Blocker_001.fcs |
