Experiment Overview
| Repository ID: | FR-FCM-ZZCA | Experiment name: | Monocyte CD4 and IFNg mRNA expression kinetics in stimulated PBMCs | MIFlowCyt score: | 100.00% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2014-03-31 - 2014-04-01 | Dataset uploaded: | May 2014 | Last updated: | Aug 2014 |
| Keywords: | [CD44] [monocyte] [PBMC] [Gene expression] [RNA Flow cytometry] | Manuscripts: | [25123411] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose of this experiment was to determine the effect of PMA/Ionocmycin stimulation on the monocyte population in PBMCs. | ||||
| Conclusion: | CD4 downregulation occurs in the lymphocyte subset. | ||||
| Comments: | Figure 3 D-L: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument was setup with CST in FACSDiva software. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0 min | PBMC Stim_NTC T0_004.fcs · PBMC Stim_T0_001.fcs |
| · 120 min | PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs |
| · 300 min | PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_NTC 5 hr_005.fcs |
| Sample Type | |
|---|---|
| · PBMC | PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs · PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_T0_001.fcs |
| · PBMC no target probe added negative control | PBMC Stim_NTC 5 hr_005.fcs · PBMC Stim_NTC T0_004.fcs |
| Conditions | |
|---|---|
| · PMA/Ionomycin Stimulation | PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs · PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_NTC 5 hr_005.fcs · PBMC Stim_NTC T0_004.fcs · PBMC Stim_T0_001.fcs |
